##################################
# NO CUT ENZYMES (  207 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AgeI        
AhdI        AluI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BsrI        
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MspI        MunI        MvaI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        ScrFI       SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tsp45I      
TspRI       Tth111I     Van91I      XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcsI          249  GAATTT               [GA]^AATT[CT]
AflIII        174  ACGTGT               A^C[GA][CT]GT
ApoI          249  GAATTT               [GA]^AATT[CT]
Asp700I       145  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI         173  CACGTG               CAC^GTG
BbvI           26  GCTGC                GCAGC(8/12)
BfaI           74  CTAG                 C^TAG
BglII         286  AGATCT               A^GATCT
BsaAI         173  CACGTG               [CT]AC^GT[GA]
BsaJI         124  CCAAGG               C^C[ACGT][ACGT]GG
BsmI           53  GCATTC               GAATGC(1/-1)
BspMI          91  ACCTGC               ACCTGC(4/8)
BsrDI         186  CATTGC               GCAATG(2/0)
BstYI         286  AGATCT               [GA]^GATC[CT]
Cac8I         157  GCAAGC               GC[ACGT]^[ACGT]GC
DdeI           46  CTAAG                C^T[ACGT]AG
EarI          213  GAAGAG               CTCTTC(1/4)
EcoT14I       124  CCAAGG               C^C[AT][AT]GG
Fnu4HI         26  GCTGC                GC^[ACGT]GC
HaeIII        240  GGCC                 GG^CC
HphI          208  GGTGA                GGTGA(8/7)
ItaI           26  GCTGC                GC^[ACGT]GC
Ksp632I       213  GAAGAG               CTCTTC(1/4)
MaeI           74  CTAG                 C^TAG
MaeII         174  ACGT                 A^CGT
MboII         213  GAAGA                GAAGA(8/7)
MflI          286  AGATCT               [GA]^GATC[CT]
MnlI           67  CCTC                 CCTC(7/6)
MseI          119  TTAA                 T^TAA
MwoI           86  GCTGTACCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI           42  GACTC                GAGTC(4/5)
PmaCI         173  CACGTG               CAC^GTG
PmlI          173  CACGTG               CAC^GTG
SfaNI          95  GCATC                GCATC(5/9)
StyI          124  CCAAGG               C^C[AT][AT]GG
TaqI          235  TCGA                 T^CGA
Tru9I         119  TTAA                 T^TAA
TseI           26  GCTGC                G^C[AT]GC
TthHB8I       235  TCGA                 T^CGA
XbaI           73  TCTAGA               T^CTAGA
XhoII         286  AGATCT               [GA]^GATC[CT]
XmnI          145  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    4 enzymes) #
###################################

DpnII         183  GATC                 ^GATC
              287  GATC                
MboI          183  GATC                 ^GATC
              287  GATC                
NdeII         183  GATC                 ^GATC
              287  GATC                
Sau3AI        183  GATC                 ^GATC
              287  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

AfaI        HinfI       RsaI        TfiI        Tsp509I