##################################
# NO CUT ENZYMES ( 144 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BseAI BsgI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FbaI FokI FseI FspI
HgaI HindIII HpaI KpnI KspI
MaeIII MamI MfeI MluI MroI
MseI MunI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp45I Tth111I TthHB8I
Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 193 GACGTC GACGT^C
AfaI 186 GTAC GT^AC
AspHI 633 GAGCTC G[AT]GC[AT]^C
AvaI 18 CCCGGG C^[CT]CG[GA]G
BalI 470 TGGCCA TGG^CCA
BbeI 775 GGCGCC GGCGC^C
BglI 443 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 890 AGATCT A^GATCT
BsaI 626 GGTCTC GGTCTC(1/5)
BsaWI 266 ACCGGA [AT]^CCGG[AT]
BsiHKAI 633 GAGCTC G[AT]GC[AT]^C
BsmBI 551 CGTCTC CGTCTC(1/5)
BsoBI 18 CCCGGG C^[CT]CG[GA]G
BstAPI 166 GCACTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EaeI 470 TGGCCA [CT]^GGCC[GA]
EarI 606 CTCTTC CTCTTC(1/4)
EcoRI 802 GAATTC G^AATTC
HincII 37 GTCAAC GT[CT]^[GA]AC
HindII 37 GTCAAC GT[CT]^[GA]AC
KasI 775 GGCGCC G^GCGCC
Ksp632I 606 CTCTTC CTCTTC(1/4)
MluNI 470 TGGCCA TGG^CCA
MscI 470 TGGCCA TGG^CCA
NarI 775 GGCGCC GG^CGCC
RsaI 186 GTAC GT^AC
SacI 633 GAGCTC GAGCT^C
SapI 605 GCTCTTC GCTCTTC(1/4)
SfoI 775 GGCGCC GGC^GCC
SmaI 18 CCCGGG CCC^GGG
TfiI 418 GATTC G^A[AT]TC
XcmI 845 CCATGGACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 18 CCCGGG C^CCGGG
XmaI 18 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 802 GAATTC [GA]^AATT[CT]
829 AAATTC
AcyI 193 GACGTC G[GA]^CG[CT]C
775 GGCGCC
AlwI 69 GGATC GGATC(4/5)
867 GGATC
ApaI 22 GGGCCC GGGCC^C
690 GGGCCC
ApoI 802 GAATTC [GA]^AATT[CT]
829 AAATTC
BfaI 652 CTAG C^TAG
685 CTAG
BsaHI 193 GACGTC G[GA]^CG[CT]C
775 GGCGCC
BseRI 154 GAGGAG GAGGAG(10/8)
208 GAGGAG
BsmAI 552 GTCTC GTCTC(1/5)
627 GTCTC
BstXI 461 CCACGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
765 CCAGAGCTATGG
BstYI 69 GGATCT [GA]^GATC[CT]
890 AGATCT
HaeII 100 AGCGCC [GA]GCGC^[CT]
775 GGCGCC
Hin1I 193 GACGTC G[GA]^CG[CT]C
775 GGCGCC
HphI 137 TCACC GGTGA(8/7)
833 TCACC
MaeI 652 CTAG C^TAG
685 CTAG
MaeII 194 ACGT A^CGT
367 ACGT
MboII 222 GAAGA GAAGA(8/7)
607 TCTTC
MflI 69 GGATCT [GA]^GATC[CT]
890 AGATCT
MslI 384 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
462 CACGGCAGTG
NcoI 671 CCATGG C^CATGG
845 CCATGG
PleI 238 GAGTC GAGTC(4/5)
502 GAGTC
PpuMI 726 GGGACCT [GA]G^G[AT]CC[CT]
809 AGGACCC
SfaNI 75 GATGC GCATC(5/9)
410 GCATC
SfcI 423 CTACAG C^T[GA][CT]AG
780 CTACAG
Tsp509I 803 AATT ^AATT
830 AATT
XhoII 69 GGATCT [GA]^GATC[CT]
890 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AocI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstNI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MboI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI StyI TseI TspRI