Restriction map for
pF1KE0762
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbeI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I FbaI FseI FspI HaeII
HincII HindII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
PacI PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 1264 GTCTAC GT^[AC][GT]AC
AccII 64 CGCG CG^CG
AcyI 135 GACGCC G[GA]^CG[CT]C
Asp700I 681 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1256 GGTCC G^G[AT]CC
BamHI 1017 GGATCC G^GATCC
BbrPI 877 CACGTG CAC^GTG
BglII 585 AGATCT A^GATCT
BpmI 366 CTCCAG CTGGAG(16/14)
BsaHI 135 GACGCC G[GA]^CG[CT]C
BsiYI 929 CCACGGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 929 CCACGGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspLU11I 161 ACATGT A^CATGT
BstAPI 975 GCAAGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1112 TTCGAA TT^CGAA
BstUI 64 CGCG CG^CG
DdeI 491 CTGAG C^T[ACGT]AG
DraII 1255 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 439 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 1255 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 224 GAATTC G^AATTC
EcoRV 757 GATATC GAT^ATC
EcoT22I 559 ATGCAT ATGCA^T
FauI 125 CCCGC CCCGC(4/6)
HgaI 135 GACGC GACGC(5/10)
Hin1I 135 GACGCC G[GA]^CG[CT]C
HindIII 265 AAGCTT A^AGCTT
MaeIII 793 GTGAC ^GT[ACGT]AC
MseI 305 TTAA T^TAA
MspA1I 130 CAGCTG C[AC]G^C[GT]G
MvnI 64 CGCG CG^CG
NsiI 559 ATGCAT ATGCA^T
NspV 1112 TTCGAA TT^CGAA
PaeR7I 850 CTCGAG C^TCGAG
PmaCI 877 CACGTG CAC^GTG
PmlI 877 CACGTG CAC^GTG
PpuMI 1255 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 5 CTGCAG CTGCA^G
PvuII 130 CAGCTG CAG^CTG
SacI 469 GAGCTC GAGCT^C
SfuI 1112 TTCGAA TT^CGAA
Tru9I 305 TTAA T^TAA
Tsp45I 793 GTGAC ^GT[GC]AC
XhoI 850 CTCGAG C^TCGAG
XmnI 681 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 224 GAATTC [GA]^AATT[CT]
1291 AAATTT
AflIII 161 ACATGT A^C[GA][CT]GT
878 ACGTGT
ApoI 224 GAATTC [GA]^AATT[CT]
1291 AAATTT
AspHI 469 GAGCTC G[AT]GC[AT]^C
733 GAGCAC
AvaI 356 CCCGAG C^[CT]CG[GA]G
850 CTCGAG
BalI 299 TGGCCA TGG^CCA
827 TGGCCA
BanI 799 GGCACC G^G[CT][GA]CC
981 GGTGCC
BanII 469 GAGCTC G[GA]GC[CT]^C
886 GAGCCC
BcnI 50 CCCGG CC^[GC]GG
726 CCGGG
BsaAI 751 TACGTG [CT]AC^GT[GA]
877 CACGTG
BsgI 382 CTGCAC GTGCAG(16/14)
436 CTGCAC
BsiHKAI 469 GAGCTC G[AT]GC[AT]^C
733 GAGCAC
BsoBI 356 CCCGAG C^[CT]CG[GA]G
850 CTCGAG
BspHI 956 TCATGA T^CATGA
1214 TCATGA
CfoI 63 GCGC GCG^C
712 GCGC
Cfr13I 764 GGGCC G^G[ACGT]CC
1256 GGTCC
DsaI 824 CCGTGG C^C[GA][CT]GG
929 CCACGG
EaeI 299 TGGCCA [CT]^GGCC[GA]
827 TGGCCA
EcoT14I 767 CCTTGG C^C[AT][AT]GG
1122 CCAAGG
HapII 51 CCGG C^CGG
726 CCGG
HhaI 63 GCGC GCG^C
712 GCGC
HinP1I 63 GCGC G^CGC
712 GCGC
HpaII 51 CCGG C^CGG
726 CCGG
MaeII 752 ACGT A^CGT
878 ACGT
MluNI 299 TGGCCA TGG^CCA
827 TGGCCA
MscI 299 TGGCCA TGG^CCA
827 TGGCCA
MslI 556 CACATGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
747 CATTTACGTG
MspI 51 CCGG C^CGG
726 CCGG
NciI 50 CCCGG CC^[GC]GG
726 CCGGG
NspI 161 ACATGT [GA]CATG^[CT]
557 ACATGC
RcaI 956 TCATGA T^CATGA
1214 TCATGA
SapI 1102 GAAGAGC GCTCTTC(1/4)
471 GCTCTTC
Sau96I 764 GGGCC G^G[ACGT]CC
1256 GGTCC
SmlI 850 CTCGAG C^T[CT][GA]AG
1048 CTCAAG
StyI 767 CCTTGG C^C[AT][AT]GG
1122 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BmyI BsaI BsaJI BseRI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I DpnII EarI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MboI MboII
MflI MnlI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
ScrFI SfaNI SfcI TaqI TfiI
TseI Tsp509I TspRI TthHB8I XcmI
XhoII