Restriction map for
pF1KE1052
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BcgI BciVI BclI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DsaI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRV EcoT22I
FbaI FseI FspI Hin1I HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccI 550 GTCTAC GT^[AC][GT]AC
Alw44I 34 GTGCAC G^TGCAC
AlwNI 423 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 34 GTGCAC G^TGCAC
AspI 189 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 60 TGGCCA TGG^CCA
BbvCI 348 CCTCAGC CCTCAGC(-5/-2)
BpmI 46 CTGGAG CTGGAG(16/14)
BsaAI 790 TACGTG [CT]AC^GT[GA]
BsmAI 1042 GAGAC GTCTC(1/5)
BsmBI 1042 GAGACG CGTCTC(1/5)
BspHI 200 TCATGA T^CATGA
BssSI 1002 CACGAG CACGAG(-5/-1)
BstEII 591 GGTGACC G^GT[ACGT]ACC
BstPI 591 GGTGACC G^GT[ACGT]ACC
Cac8I 258 GCTGGC GC[ACGT]^[ACGT]GC
Cfr13I 808 GGCCC G^G[ACGT]CC
DrdI 594 GACCGTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 591 GGTGACC G^GT[ACGT]ACC
EcoT14I 638 CCAAGG C^C[AT][AT]GG
MaeII 791 ACGT A^CGT
MluNI 60 TGGCCA TGG^CCA
MscI 60 TGGCCA TGG^CCA
MseI 335 TTAA T^TAA
PflFI 189 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 384 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 200 TCATGA T^CATGA
Sau96I 808 GGCCC G^G[ACGT]CC
StuI 946 AGGCCT AGG^CCT
StyI 638 CCAAGG C^C[AT][AT]GG
Tru9I 335 TTAA T^TAA
Tth111I 189 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
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AfaI 523 GTAC GT^AC
777 GTAC
Aor51HI 42 AGCGCT AGC^GCT
368 AGCGCT
BanII 480 GGGCTC G[GA]GC[CT]^C
892 GAGCCC
BbsI 1033 GAAGAC GAAGAC(2/6)
23 GTCTTC
BcnI 561 CCCGG CC^[GC]GG
749 CCGGG
BglII 757 AGATCT A^GATCT
764 AGATCT
BpuAI 1033 GAAGAC GAAGAC(2/6)
23 GTCTTC
BsgI 876 CTGCAC GTGCAG(16/14)
433 GTGCAG
BstF5I 51 GGATG GGATG(2/0)
939 GGATG
CfoI 43 GCGC GCG^C
369 GCGC
Eco47III 42 AGCGCT AGC^GCT
368 AGCGCT
EcoRI 418 GAATTC G^AATTC
628 GAATTC
FokI 51 GGATG GGATG(9/13)
939 GGATG
HaeII 42 AGCGCT [GA]GCGC^[CT]
368 AGCGCT
HapII 562 CCGG C^CGG
749 CCGG
HhaI 43 GCGC GCG^C
369 GCGC
HinP1I 43 GCGC G^CGC
369 GCGC
HincII 381 GTTGAC GT[CT]^[GA]AC
391 GTCAAC
HindII 381 GTTGAC GT[CT]^[GA]AC
391 GTCAAC
HpaII 562 CCGG C^CGG
749 CCGG
MspI 562 CCGG C^CGG
749 CCGG
NciI 561 CCCGG CC^[GC]GG
749 CCGGG
NlaIII 201 CATG CATG^
678 CATG
PflMI 649 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
720 CCAGGGTGTGG
PvuII 423 CAGCTG CAG^CTG
781 CAGCTG
RsaI 523 GTAC GT^AC
777 GTAC
SfaNI 204 GATGC GCATC(5/9)
1051 GATGC
Van91I 649 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
720 CCAGGGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI BbvI BmyI BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstNI BstYI DdeI DpnII
EaeI EcoRII FauI Fnu4HI HaeIII
HgaI HinfI HphI ItaI MaeIII
MboI MboII MflI MnlI MslI
MspA1I MvaI MwoI NdeII NlaIV
PleI PstI Sau3AI ScrFI SfcI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII