Restriction map for
pF1KE1051
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AvaI
AvrII BamHI BcgI BciVI BclI
BfaI BfrI BlnI BlpI BpmI
Bpu1102I BsaBI BsaI BseRI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI MaeI MamI MfeI
MluI MseI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 133 GTCTAC GT^[AC][GT]AC
AccIII 291 TCCGGA T^CCGGA
Asp700I 1251 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1153 GAGCAC G[AT]GC[AT]^C
BbeI 711 GGCGCC GGCGC^C
BbrPI 589 CACGTG CAC^GTG
BbsI 718 GTCTTC GAAGAC(2/6)
BglII 237 AGATCT A^GATCT
BpuAI 718 GTCTTC GAAGAC(2/6)
BsaAI 589 CACGTG [CT]AC^GT[GA]
BsaWI 291 TCCGGA [AT]^CCGG[AT]
BseAI 291 TCCGGA T^CCGGA
BsgI 352 CTGCAC GTGCAG(16/14)
BsiHKAI 1153 GAGCAC G[AT]GC[AT]^C
BsmAI 1179 GAGAC GTCTC(1/5)
BsmI 321 GAATGC GAATGC(1/-1)
BspEI 291 TCCGGA T^CCGGA
BspMI 1090 ACCTGC ACCTGC(4/8)
BssHII 86 GCGCGC G^CGCGC
BssSI 1048 CACGAG CACGAG(-5/-1)
BstXI 485 CCAGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI 351 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HinfI 733 GAGTC G^A[ACGT]TC
KasI 711 GGCGCC G^GCGCC
KspI 25 CCGCGG CCGC^GG
MroI 291 TCCGGA T^CCGGA
NarI 711 GGCGCC GG^CGCC
NcoI 431 CCATGG C^CATGG
PflMI 420 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 733 GAGTC GAGTC(4/5)
PmaCI 589 CACGTG CAC^GTG
PmlI 589 CACGTG CAC^GTG
PstI 538 CTGCAG CTGCA^G
SacII 25 CCGCGG CCGC^GG
SapI 963 GCTCTTC GCTCTTC(1/4)
SfoI 711 GGCGCC GGC^GCC
SphI 872 GCATGC GCATG^C
Tsp509I 244 AATT ^AATT
Van91I 420 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1251 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 795 GTAC GT^AC
858 GTAC
AlwI 81 GATCC GGATC(4/5)
1010 GGATC
AspI 1063 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1276 GACATCGTC
AviII 251 TGCGCA TGC^GCA
324 TGCGCA
BalI 428 TGGCCA TGG^CCA
509 TGGCCA
BanII 347 GAGCCC G[GA]GC[CT]^C
1102 GAGCCC
BbvCI 1236 CCTCAGC CCTCAGC(-5/-2)
199 GCTGAGG
BglI 179 GCCTCATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
188 GCCACCACGGC
BstYI 80 AGATCC [GA]^GATC[CT]
237 AGATCT
EarI 964 CTCTTC CTCTTC(1/4)
1162 CTCTTC
EcoT14I 431 CCATGG C^C[AT][AT]GG
1034 CCAAGG
FspI 251 TGCGCA TGC^GCA
324 TGCGCA
Ksp632I 964 CTCTTC CTCTTC(1/4)
1162 CTCTTC
MaeIII 703 GTGAC ^GT[ACGT]AC
1304 GTGAC
MflI 80 AGATCC [GA]^GATC[CT]
237 AGATCT
MluNI 428 TGGCCA TGG^CCA
509 TGGCCA
MscI 428 TGGCCA TGG^CCA
509 TGGCCA
NspI 860 ACATGC [GA]CATG^[CT]
872 GCATGC
PflFI 1063 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1276 GACATCGTC
PvuII 328 CAGCTG CAG^CTG
1078 CAGCTG
RsaI 795 GTAC GT^AC
858 GTAC
StyI 431 CCATGG C^C[AT][AT]GG
1034 CCAAGG
Tsp45I 703 GTGAC ^GT[GC]AC
1304 GTGAC
Tth111I 1063 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1276 GACATCGTC
XcmI 431 CCATGGAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1088 CCACCTGCAACGTGG
XhoII 80 AGATCC [GA]^GATC[CT]
237 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaII BanI BbvI BcnI BmyI
BsaHI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MaeII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PpuMI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TseI TspRI TthHB8I