Restriction map for
pF1KE1050
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbeI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaBI
BseAI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HinfI
HpaI KasI KpnI MaeI MamI
MfeI MluI MroI MseI MunI
NarI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SalI SapI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI SspBI SspI SwaI TfiI
Tru9I Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AcyI 280 GACGCC G[GA]^CG[CT]C
AlwNI 1380 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 52 CCTCAGG CC^T[ACGT]AGG
BamHI 1046 GGATCC G^GATCC
BlpI 297 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 297 GCTGAGC GC^T[ACGT]AGC
BsaHI 280 GACGCC G[GA]^CG[CT]C
BsaI 687 GGTCTC GGTCTC(1/5)
BsmBI 437 CGTCTC CGTCTC(1/5)
BspMI 1090 ACCTGC ACCTGC(4/8)
BsrBI 427 CCGCTC CCGCTC(-3/-3)
BstAPI 1370 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 831 CCAGTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 52 CCTCAGG CC^T[ACGT]AGG
CelII 297 GCTGAGC GC^T[ACGT]AGC
Eco81I 52 CCTCAGG CC^T[ACGT]AGG
EcoT14I 213 CCATGG C^C[AT][AT]GG
HaeII 1381 AGCGCC [GA]GCGC^[CT]
Hin1I 280 GACGCC G[GA]^CG[CT]C
MslI 1089 CACCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 195 GCCGGC GCC^GGC
NcoI 213 CCATGG C^CATGG
NdeI 365 CATATG CA^TATG
NgoMIV 195 GCCGGC G^CCGGC
NspI 925 ACATGC [GA]CATG^[CT]
PpuMI 208 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 1399 GAGCTC GAGCT^C
SbfI 697 CCTGCAGG CCTGCA^GG
Sse8387I 697 CCTGCAGG CCTGCA^GG
StyI 213 CCATGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 220 GTAC GT^AC
822 GTAC
AvaI 81 CCCGGG C^[CT]CG[GA]G
585 CCCGGG
BalI 828 TGGCCA TGG^CCA
998 TGGCCA
BanI 76 GGCACC G^G[CT][GA]CC
735 GGTGCC
BbrPI 901 CACGTG CAC^GTG
1020 CACGTG
BsaWI 180 ACCGGA [AT]^CCGG[AT]
1043 ACCGGA
BsmFI 177 GGGAC GGGAC(10/14)
210 GTCCC
BsoBI 81 CCCGGG C^[CT]CG[GA]G
585 CCCGGG
BsrFI 93 ACCGGC [GA]^CCGG[CT]
195 GCCGGC
BsrI 529 CCAGT ACTGG(1/-1)
831 CCAGT
BstYI 1046 GGATCC [GA]^GATC[CT]
1151 AGATCC
CfoI 1382 GCGC GCG^C
1389 GCGC
Cfr10I 93 ACCGGC [GA]^CCGG[CT]
195 GCCGGC
EarI 704 GAAGAG CTCTTC(1/4)
710 GAAGAG
EcoNI 601 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1222 CCTGAGCCAGG
HhaI 1382 GCGC GCG^C
1389 GCGC
HinP1I 1382 GCGC G^CGC
1389 GCGC
Ksp632I 704 GAAGAG CTCTTC(1/4)
710 GAAGAG
KspI 72 CCGCGG CCGC^GG
1107 CCGCGG
MflI 1046 GGATCC [GA]^GATC[CT]
1151 AGATCC
MluNI 828 TGGCCA TGG^CCA
998 TGGCCA
MscI 828 TGGCCA TGG^CCA
998 TGGCCA
PmaCI 901 CACGTG CAC^GTG
1020 CACGTG
PmlI 901 CACGTG CAC^GTG
1020 CACGTG
RsaI 220 GTAC GT^AC
822 GTAC
SacII 72 CCGCGG CCGC^GG
1107 CCGCGG
ScaI 219 AGTACT AGT^ACT
821 AGTACT
SmaI 81 CCCGGG CCC^GGG
585 CCCGGG
TaqI 1115 TCGA T^CGA
1266 TCGA
Tsp509I 133 AATT ^AATT
1251 AATT
TthHB8I 1115 TCGA T^CGA
1266 TCGA
XhoII 1046 GGATCC [GA]^GATC[CT]
1151 AGATCC
XmaCI 81 CCCGGG C^CCGGG
585 CCCGGG
XmaI 81 CCCGGG C^CCGGG
585 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AluI AlwI ApaI
AspHI AvaII BanII BbvI BcnI
BglI BmyI BpmI BsaAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PstI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI TseI
Tsp45I TspRI