Restriction map for
pF1KE1048
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BglI BglII BlnI BsaAI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI FauI FbaI
FseI FspI HaeII HapII HhaI
HinP1I HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MluI MroI
MspA1I MspI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccI 655 GTAGAC GT^[AC][GT]AC
AcyI 1109 GGCGTC G[GA]^CG[CT]C
AfaI 978 GTAC GT^AC
AflII 931 CTTAAG C^TTAAG
AseI 262 ATTAAT AT^TAAT
AsnI 262 ATTAAT AT^TAAT
Asp700I 72 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1182 GAGCAC G[AT]GC[AT]^C
AspI 923 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 883 GGTCC G^G[AT]CC
BalI 1155 TGGCCA TGG^CCA
BanI 609 GGCACC G^G[CT][GA]CC
BbsI 624 GAAGAC GAAGAC(2/6)
BfaI 788 CTAG C^TAG
BfrI 931 CTTAAG C^TTAAG
BlpI 34 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 34 GCTAAGC GC^T[ACGT]AGC
BpuAI 624 GAAGAC GAAGAC(2/6)
BsaBI 1067 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1109 GGCGTC G[GA]^CG[CT]C
BsaI 244 GAGACC GGTCTC(1/5)
BsgI 1085 CTGCAC GTGCAG(16/14)
BsiHKAI 1182 GAGCAC G[AT]GC[AT]^C
BsmFI 889 GTCCC GGGAC(10/14)
BsmI 744 GAATGC GAATGC(1/-1)
BspMI 651 GCAGGT ACCTGC(4/8)
BstEII 1051 GGTCACC G^GT[ACGT]ACC
BstPI 1051 GGTCACC G^GT[ACGT]ACC
BstXI 1040 CCATTGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 34 GCTAAGC GC^T[ACGT]AGC
DsaI 479 CCATGG C^C[GA][CT]GG
EaeI 1155 TGGCCA [CT]^GGCC[GA]
EcoO65I 1051 GGTCACC G^GT[ACGT]ACC
EcoRV 421 GATATC GAT^ATC
EcoT22I 746 ATGCAT ATGCA^T
HgaI 1110 GCGTC GACGC(5/10)
Hin1I 1109 GGCGTC G[GA]^CG[CT]C
HincII 151 GTTGAC GT[CT]^[GA]AC
HindII 151 GTTGAC GT[CT]^[GA]AC
MaeI 788 CTAG C^TAG
MamI 1067 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 614 CAATTG C^AATTG
MluNI 1155 TGGCCA TGG^CCA
MscI 1155 TGGCCA TGG^CCA
MslI 480 CATGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 614 CAATTG C^AATTG
NcoI 479 CCATGG C^CATGG
NsiI 746 ATGCAT ATGCA^T
PflFI 923 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 975 CCAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 927 GAGTC GAGTC(4/5)
PshBI 262 ATTAAT AT^TAAT
RsaI 978 GTAC GT^AC
ScaI 977 AGTACT AGT^ACT
SexAI 1091 ACCTGGT A^CC[AT]GGT
SmlI 931 CTTAAG C^T[CT][GA]AG
SphI 663 GCATGC GCATG^C
StuI 63 AGGCCT AGG^CCT
TaqI 1113 TCGA T^CGA
Tth111I 923 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1113 TCGA T^CGA
Van91I 975 CCAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 72 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 100 GAATTT [GA]^AATT[CT]
314 AAATTT
ApoI 100 GAATTT [GA]^AATT[CT]
314 AAATTT
BmyI 905 GGGCAC G[AGT]GC[ACT]^C
1182 GAGCAC
BpmI 707 CTCCAG CTGGAG(16/14)
770 CTGGAG
BseRI 993 GAGGAG GAGGAG(10/8)
1034 GAGGAG
BsiYI 975 CCAGTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1057 CCACTGGGAGG
BslI 975 CCAGTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1057 CCACTGGGAGG
BsmAI 244 GAGAC GTCTC(1/5)
136 GTCTC
Bsp1286I 905 GGGCAC G[AGT]GC[ACT]^C
1182 GAGCAC
BssKI 684 CCTGG ^CC[ACGT]GG
1092 CCTGG
BstAPI 484 GCAATGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
739 GCAGGGAATGC
BstF5I 304 GGATG GGATG(2/0)
856 GGATG
BstNI 684 CCTGG CC^[AT]GG
1092 CCTGG
BstYI 1002 GGATCT [GA]^GATC[CT]
1066 GGATCT
Cfr13I 671 GGCCC G^G[ACGT]CC
883 GGTCC
DdeI 35 CTAAG C^T[ACGT]AG
925 CTGAG
EcoRII 684 CCTGG ^CC[AT]GG
1092 CCTGG
FokI 304 GGATG GGATG(9/13)
856 GGATG
HindIII 409 AAGCTT A^AGCTT
962 AAGCTT
MflI 1002 GGATCT [GA]^GATC[CT]
1066 GGATCT
MvaI 684 CCTGG CC^[AT]GG
1092 CCTGG
Sau96I 671 GGCCC G^G[ACGT]CC
883 GGTCC
ScrFI 684 CCTGG CC^[ACGT]GG
1092 CCTGG
XhoII 1002 GGATCT [GA]^GATC[CT]
1066 GGATCT
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI AlwI BbvI BsaJI BsrDI
BsrI Cac8I DpnII EcoT14I Fnu4HI
HaeIII HinfI HphI ItaI MaeIII
MboI MboII MnlI MseI MwoI
NdeII NlaIII NlaIV NspI PstI
Sau3AI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI