Restriction map for
pF1KE3209
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII AccIII AclI AcsI AflII
AgeI AhdI Alw44I AocI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsgI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI DraI DraIII DrdI
Eam1105I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI MfeI MluI MroI MseI
MunI NdeI NheI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SacI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tru9I
Tsp509I XbaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 1136 GGTACC G^GTACC
AccI 325 GTCGAC GT^[AC][GT]AC
AcyI 579 GGCGCC G[GA]^CG[CT]C
AflIII 1406 ACATGT A^C[GA][CT]GT
Aor51HI 432 AGCGCT AGC^GCT
Asp718I 1136 GGTACC G^GTACC
AspHI 1125 GTGCTC G[AT]GC[AT]^C
AspI 217 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 752 TGGCCA TGG^CCA
BbeI 579 GGCGCC GGCGC^C
BbsI 1077 GAAGAC GAAGAC(2/6)
BfaI 733 CTAG C^TAG
BpuAI 1077 GAAGAC GAAGAC(2/6)
BsaBI 1369 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 579 GGCGCC G[GA]^CG[CT]C
BsaI 815 GAGACC GGTCTC(1/5)
BsiHKAI 1125 GTGCTC G[AT]GC[AT]^C
BsmAI 815 GAGAC GTCTC(1/5)
BspLU11I 1406 ACATGT A^CATGT
BsrBI 14 CCGCTC CCGCTC(-3/-3)
BsrI 883 ACTGG ACTGG(1/-1)
BstYI 162 GGATCT [GA]^GATC[CT]
CpoI 293 CGGTCCG CG^G[AT]CCG
Eco47III 432 AGCGCT AGC^GCT
EcoNI 597 CCTGTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 579 GGCGCC G[GA]^CG[CT]C
HincII 325 GTCGAC GT[CT]^[GA]AC
HindII 325 GTCGAC GT[CT]^[GA]AC
HphI 1186 TCACC GGTGA(8/7)
KasI 579 GGCGCC G^GCGCC
KpnI 1136 GGTACC GGTAC^C
MaeI 733 CTAG C^TAG
MaeII 196 ACGT A^CGT
MaeIII 781 GTGAC ^GT[ACGT]AC
MamI 1369 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 162 GGATCT [GA]^GATC[CT]
MluNI 752 TGGCCA TGG^CCA
MscI 752 TGGCCA TGG^CCA
MslI 1407 CATGTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 579 GGCGCC GG^CGCC
NcoI 569 CCATGG C^CATGG
PflFI 217 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 213 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 370 GACTC GAGTC(4/5)
RsrII 293 CGGTCCG CG^G[AT]CCG
SalI 325 GTCGAC G^TCGAC
SapI 516 GCTCTTC GCTCTTC(1/4)
SbfI 147 CCTGCAGG CCTGCA^GG
SfoI 579 GGCGCC GGC^GCC
Sse8387I 147 CCTGCAGG CCTGCA^GG
Tsp45I 781 GTGAC ^GT[GC]AC
Tth111I 217 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 213 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 162 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 381 GATCC GGATC(4/5)
162 GGATC
BpmI 166 CTCCAG CTGGAG(16/14)
892 CTCCAG
BseRI 658 CTCCTC GAGGAG(10/8)
682 GAGGAG
BspMI 512 ACCTGC ACCTGC(4/8)
1056 GCAGGT
BsrFI 94 GCCGGC [GA]^CCGG[CT]
260 GCCGGC
BssHII 269 GCGCGC G^CGCGC
933 GCGCGC
Cfr10I 94 GCCGGC [GA]^CCGG[CT]
260 GCCGGC
EarI 517 CTCTTC CTCTTC(1/4)
1360 CTCTTC
HgaI 184 GACGC GACGC(5/10)
783 GACGC
Ksp632I 517 CTCTTC CTCTTC(1/4)
1360 CTCTTC
NaeI 94 GCCGGC GCC^GGC
260 GCCGGC
NgoMIV 94 GCCGGC G^CCGGC
260 GCCGGC
NotI 133 GCGGCCGC GC^GGCCGC
187 GCGGCCGC
PaeR7I 428 CTCGAG C^TCGAG
436 CTCGAG
PstI 148 CTGCAG CTGCA^G
787 CTGCAG
SfcI 148 CTGCAG C^T[GA][CT]AG
787 CTGCAG
SmlI 428 CTCGAG C^T[CT][GA]AG
436 CTCGAG
TfiI 676 GATTC G^A[AT]TC
1374 GAATC
XhoI 428 CTCGAG C^TCGAG
436 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AvaI AvaII BanI BanII
BbvI BcnI BglI BmyI BsaJI
BsiEI BsiYI BslI BsmFI BsoBI
Bsp1286I BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI KspI MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PpuMI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SmaI StyI
TaqI TseI TspRI TthHB8I XcmI
XmaCI XmaI