Restriction map for
pF1KE1040
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# NO CUT ENZYMES ( 122 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglI BlnI BpuAI BsaBI BseAI
BsgI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI Tth111I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 339 GTCTAC GT^[AC][GT]AC
AccII 8 CGCG CG^CG
AcyI 867 GGCGTC G[GA]^CG[CT]C
AhdI 1123 GACTACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1123 GACTACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1003 CCCGAG C^[CT]CG[GA]G
BanII 3 GAGCTC G[GA]GC[CT]^C
BcnI 699 CCGGG CC^[GC]GG
BfaI 88 CTAG C^TAG
BglII 90 AGATCT A^GATCT
BsaAI 1126 TACGTG [CT]AC^GT[GA]
BsaHI 867 GGCGTC G[GA]^CG[CT]C
BseRI 1231 CTCCTC GAGGAG(10/8)
BsiEI 804 CGATCG CG[GA][CT]^CG
BsiWI 914 CGTACG C^GTACG
BsoBI 1003 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1094 TGTACA T^GTACA
BsrBI 18 GAGCGG CCGCTC(-3/-3)
BsrDI 672 CATTGC GCAATG(2/0)
BsrFI 864 GCCGGC [GA]^CCGG[CT]
BsrGI 1094 TGTACA T^GTACA
BssSI 210 CTCGTG CACGAG(-5/-1)
BstUI 8 CGCG CG^CG
CfoI 79 GCGC GCG^C
Cfr10I 864 GCCGGC [GA]^CCGG[CT]
Eam1105I 1123 GACTACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1176 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 78 AGCGCC [GA]GCGC^[CT]
HgaI 868 GCGTC GACGC(5/10)
HhaI 79 GCGC GCG^C
Hin1I 867 GGCGTC G[GA]^CG[CT]C
HinP1I 79 GCGC G^CGC
HincII 394 GTCAAC GT[CT]^[GA]AC
HindII 394 GTCAAC GT[CT]^[GA]AC
HindIII 409 AAGCTT A^AGCTT
HphI 1324 GGTGA GGTGA(8/7)
MaeI 88 CTAG C^TAG
MslI 228 CATGGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 8 CGCG CG^CG
NaeI 864 GCCGGC GCC^GGC
NciI 699 CCGGG CC^[GC]GG
NgoMIV 864 GCCGGC G^CCGGC
PflMI 474 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1164 CTGCAG CTGCA^G
PvuI 804 CGATCG CGAT^CG
PvuII 109 CAGCTG CAG^CTG
SacI 3 GAGCTC GAGCT^C
SexAI 1181 ACCAGGT A^CC[AT]GGT
SgrAI 863 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1305 CTTGAG C^T[CT][GA]AG
SspBI 1094 TGTACA T^GTACA
Van91I 474 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 87 TCTAGA T^CTAGA
XcmI 1287 CCAGATCCCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 48 ACACGT A^C[GA][CT]GT
1127 ACGTGT
AlwNI 331 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
475 CAGCGGCTG
AspHI 3 GAGCTC G[AT]GC[AT]^C
484 GAGCAC
BamHI 284 GGATCC G^GATCC
497 GGATCC
BcgI 44 GCATACACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
606 GCACTTTGTTCG
BmyI 3 GAGCTC G[AGT]GC[ACT]^C
484 GAGCAC
BpmI 481 CTGGAG CTGGAG(16/14)
502 CTGGAG
BsaI 261 GGTCTC GGTCTC(1/5)
1158 GGTCTC
BsaWI 119 ACCGGA [AT]^CCGG[AT]
494 ACCGGA
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
484 GAGCAC
Bsp1286I 3 GAGCTC G[AGT]GC[ACT]^C
484 GAGCAC
BspMI 101 ACCTGC ACCTGC(4/8)
446 ACCTGC
DraII 1007 AGGACCT [GA]G^G[ACGT]CC[CT]
1313 AGGACCC
DraIII 49 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
1319 CACTGGGTG
DrdI 1030 GACATGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1150 GACAGCAAGGTC
DsaI 1019 CCGTGG C^C[GA][CT]GG
1075 CCGTGG
EarI 724 CTCTTC CTCTTC(1/4)
1047 CTCTTC
EcoO109I 1007 AGGACCT [GA]G^G[ACGT]CC[CT]
1313 AGGACCC
Ksp632I 724 CTCTTC CTCTTC(1/4)
1047 CTCTTC
PleI 208 GACTC GAGTC(4/5)
392 GAGTC
PpuMI 1007 AGGACCT [GA]G^G[AT]CC[CT]
1313 AGGACCC
TfiI 333 GATTC G^A[AT]TC
619 GAATC
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BlpI Bpu1102I BsaJI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII