Restriction map for
pF1KE1036
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AviII BamHI
BbvCI BcgI BclI BfrI BglII
BsaBI BsaI BsiEI BsiHKAI BsiWI
BspDI BspHI BspLU11I BssHII BssSI
Bst1107I BstBI BstYI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MamI
MfeI MflI MluI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SapI SbfI
ScaI SfiI SfuI SgrAI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 472 GTCGAC GT^[AC][GT]AC
AccII 1233 CGCG CG^CG
AhdI 1318 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1312 CCTGAGG CC^T[ACGT]AGG
AspEI 1318 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 466 TGGCCA TGG^CCA
BbrPI 1083 CACGTG CAC^GTG
BsmI 861 GAATGC GAATGC(1/-1)
Bsp1407I 245 TGTACA T^GTACA
BsrBI 1500 CCGCTC CCGCTC(-3/-3)
BsrGI 245 TGTACA T^GTACA
BstAPI 103 GCAGTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1194 GGTGACC G^GT[ACGT]ACC
BstPI 1194 GGTGACC G^GT[ACGT]ACC
BstUI 1233 CGCG CG^CG
Bsu36I 1312 CCTGAGG CC^T[ACGT]AGG
Eam1105I 1318 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1312 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1194 GGTGACC G^GT[ACGT]ACC
HincII 472 GTCGAC GT[CT]^[GA]AC
HindII 472 GTCGAC GT[CT]^[GA]AC
MluNI 466 TGGCCA TGG^CCA
MscI 466 TGGCCA TGG^CCA
MseI 599 TTAA T^TAA
MvnI 1233 CGCG CG^CG
NcoI 989 CCATGG C^CATGG
NspI 165 ACATGC [GA]CATG^[CT]
PaeR7I 512 CTCGAG C^TCGAG
PmaCI 1083 CACGTG CAC^GTG
PmlI 1083 CACGTG CAC^GTG
PpuMI 1257 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1480 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 472 GTCGAC G^TCGAC
SmaI 1376 CCCGGG CCC^GGG
SnaBI 135 TACGTA TAC^GTA
SspBI 245 TGTACA T^GTACA
StuI 1424 AGGCCT AGG^CCT
TfiI 930 GAATC G^A[AT]TC
Tru9I 599 TTAA T^TAA
Tsp509I 1452 AATT ^AATT
XhoI 512 CTCGAG C^TCGAG
XmaCI 1376 CCCGGG C^CCGGG
XmaI 1376 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 458 TCCGGA T^CCGGA
1274 TCCGGA
AlwI 1122 GATCC GGATC(4/5)
1272 GATCC
AvaI 512 CTCGAG C^[CT]CG[GA]G
1376 CCCGGG
AvrII 24 CCTAGG C^CTAGG
645 CCTAGG
BbeI 650 GGCGCC GGCGC^C
749 GGCGCC
BbsI 414 GTCTTC GAAGAC(2/6)
1397 GTCTTC
BfaI 25 CTAG C^TAG
646 CTAG
BglI 335 GCCTCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1081 GCCACGTGGGC
BlnI 24 CCTAGG C^CTAGG
645 CCTAGG
BlpI 12 GCTGAGC GC^T[ACGT]AGC
820 GCTGAGC
Bpu1102I 12 GCTGAGC GC^T[ACGT]AGC
820 GCTGAGC
BpuAI 414 GTCTTC GAAGAC(2/6)
1397 GTCTTC
BsaAI 135 TACGTA [CT]AC^GT[GA]
1083 CACGTG
BsaWI 458 TCCGGA [AT]^CCGG[AT]
1274 TCCGGA
BseAI 458 TCCGGA T^CCGGA
1274 TCCGGA
BsmBI 888 CGTCTC CGTCTC(1/5)
91 GAGACG
BsoBI 512 CTCGAG C^[CT]CG[GA]G
1376 CCCGGG
BspEI 458 TCCGGA T^CCGGA
1274 TCCGGA
BspMI 680 ACCTGC ACCTGC(4/8)
322 GCAGGT
BsrFI 371 ACCGGC [GA]^CCGG[CT]
806 ACCGGC
CelII 12 GCTGAGC GC^T[ACGT]AGC
820 GCTGAGC
Cfr10I 371 ACCGGC [GA]^CCGG[CT]
806 ACCGGC
DsaI 989 CCATGG C^C[GA][CT]GG
1304 CCACGG
EarI 736 GAAGAG CTCTTC(1/4)
1474 GAAGAG
HaeII 650 GGCGCC [GA]GCGC^[CT]
749 GGCGCC
KasI 650 GGCGCC G^GCGCC
749 GGCGCC
Ksp632I 736 GAAGAG CTCTTC(1/4)
1474 GAAGAG
MaeI 25 CTAG C^TAG
646 CTAG
MaeII 136 ACGT A^CGT
1084 ACGT
MroI 458 TCCGGA T^CCGGA
1274 TCCGGA
NarI 650 GGCGCC GG^CGCC
749 GGCGCC
PflMI 122 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1017 CCACCGCCTGG
PleI 61 GACTC GAGTC(4/5)
508 GAGTC
SexAI 629 ACCTGGT A^CC[AT]GGT
1145 ACCTGGT
SfoI 650 GGCGCC GGC^GCC
749 GGCGCC
SmlI 261 CTTGAG C^T[CT][GA]AG
512 CTCGAG
Tsp45I 106 GTCAC ^GT[GC]AC
1195 GTGAC
Van91I 122 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1017 CCACCGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
ApaI AvaII BanI BanII BbvI
BciVI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI TspRI TthHB8I XcmI