Restriction map for
pF1KB6948
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# NO CUT ENZYMES ( 186 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfaNI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AciI 121 GCGG CCGC(-3/-1)
AcsI 436 AAATTT [GA]^AATT[CT]
AfaI 386 GTAC GT^AC
AocI 304 CCTGAGG CC^T[ACGT]AGG
ApoI 436 AAATTT [GA]^AATT[CT]
BalI 294 TGGCCA TGG^CCA
BamHI 221 GGATCC G^GATCC
BbvI 57 GCAGC GCAGC(8/12)
BseRI 307 GAGGAG GAGGAG(10/8)
BsgI 569 GTGCAG GTGCAG(16/14)
BssKI 225 CCTGG ^CC[ACGT]GG
BstBI 390 TTCGAA TT^CGAA
BstNI 225 CCTGG CC^[AT]GG
BstXI 457 CCATCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 304 CCTGAGG CC^T[ACGT]AGG
Cac8I 378 GCTGGC GC[ACGT]^[ACGT]GC
DsaI 499 CCATGG C^C[GA][CT]GG
EaeI 294 TGGCCA [CT]^GGCC[GA]
EarI 367 GAAGAG CTCTTC(1/4)
Eco81I 304 CCTGAGG CC^T[ACGT]AGG
EcoRII 225 CCTGG ^CC[AT]GG
Fnu4HI 57 GCAGC GC^[ACGT]GC
ItaI 57 GCAGC GC^[ACGT]GC
Ksp632I 367 GAAGAG CTCTTC(1/4)
MaeII 285 ACGT A^CGT
MluNI 294 TGGCCA TGG^CCA
MscI 294 TGGCCA TGG^CCA
MslI 458 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 225 CCTGG CC^[AT]GG
MwoI 77 GCAATAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 499 CCATGG C^CATGG
NlaIV 221 GGATCC GG[ACGT]^[ACGT]CC
NspV 390 TTCGAA TT^CGAA
PleI 544 GACTC GAGTC(4/5)
RsaI 386 GTAC GT^AC
ScrFI 225 CCTGG CC^[ACGT]GG
SfcI 413 CTATAG C^T[GA][CT]AG
SfuI 390 TTCGAA TT^CGAA
TfiI 33 GAATC G^A[AT]TC
TseI 57 GCAGC G^C[AT]GC
Tsp45I 505 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BpmI 200 CTCCAG CTGGAG(16/14)
399 CTCCAG
BsrI 401 CCAGT ACTGG(1/-1)
603 CCAGT
BstF5I 458 CATCC GGATG(2/0)
496 CATCC
BstYI 221 GGATCC [GA]^GATC[CT]
610 GGATCT
DpnII 222 GATC ^GATC
611 GATC
EcoT14I 9 CCAAGG C^C[AT][AT]GG
499 CCATGG
FokI 458 CATCC GGATG(9/13)
496 CATCC
HindIII 140 AAGCTT A^AGCTT
640 AAGCTT
HinfI 33 GAATC G^A[ACGT]TC
544 GACTC
HphI 504 GGTGA GGTGA(8/7)
454 TCACC
MboI 222 GATC ^GATC
611 GATC
MflI 221 GGATCC [GA]^GATC[CT]
610 GGATCT
NdeII 222 GATC ^GATC
611 GATC
Sau3AI 222 GATC ^GATC
611 GATC
StyI 9 CCAAGG C^C[AT][AT]GG
499 CCATGG
TaqI 391 TCGA T^CGA
588 TCGA
TthHB8I 391 TCGA T^CGA
588 TCGA
XhoII 221 GGATCC [GA]^GATC[CT]
610 GGATCT
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AluI AlwI BsaJI DdeI HaeIII
MaeIII MboII MnlI MseI NlaIII
Tru9I Tsp509I TspRI