Restriction map for
pF1KE1018
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmFI BsoBI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
Bst1107I BstAPI BstBI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MspA1I
MunI MvnI MwoI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 665 GTCTAC GT^[AC][GT]AC
AcsI 1601 GAATTC [GA]^AATT[CT]
ApoI 1601 GAATTC [GA]^AATT[CT]
AspHI 398 GTGCTC G[AT]GC[AT]^C
AvaII 146 GGACC G^G[AT]CC
BcnI 252 CCCGG CC^[GC]GG
BfaI 76 CTAG C^TAG
BmyI 398 GTGCTC G[AGT]GC[ACT]^C
BseRI 115 GAGGAG GAGGAG(10/8)
BsiHKAI 398 GTGCTC G[AT]GC[AT]^C
BsmBI 158 GAGACG CGTCTC(1/5)
Bsp1286I 398 GTGCTC G[AGT]GC[ACT]^C
BspHI 411 TCATGA T^CATGA
BspMI 204 GCAGGT ACCTGC(4/8)
BsrDI 1463 GCAATG GCAATG(2/0)
BssSI 401 CTCGTG CACGAG(-5/-1)
BstEII 207 GGTTACC G^GT[ACGT]ACC
BstPI 207 GGTTACC G^GT[ACGT]ACC
Cac8I 1711 GCATGC GC[ACGT]^[ACGT]GC
Cfr13I 146 GGACC G^G[ACGT]CC
DpnII 1078 GATC ^GATC
DraII 145 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1716 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1875 CCATGG C^C[GA][CT]GG
EarI 1880 GAAGAG CTCTTC(1/4)
EcoO109I 145 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 207 GGTTACC G^GT[ACGT]ACC
EcoRI 1601 GAATTC G^AATTC
EcoT22I 1709 ATGCAT ATGCA^T
HapII 253 CCGG C^CGG
HpaII 253 CCGG C^CGG
Ksp632I 1880 GAAGAG CTCTTC(1/4)
MaeI 76 CTAG C^TAG
MaeII 161 ACGT A^CGT
MboI 1078 GATC ^GATC
MseI 680 TTAA T^TAA
MslI 325 CATCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 253 CCGG C^CGG
NciI 252 CCCGG CC^[GC]GG
NcoI 1875 CCATGG C^CATGG
NdeII 1078 GATC ^GATC
NlaIV 146 GGACCC GG[ACGT]^[ACGT]CC
NsiI 1709 ATGCAT ATGCA^T
PpuMI 145 AGGACCC [GA]G^G[AT]CC[CT]
PstI 533 CTGCAG CTGCA^G
RcaI 411 TCATGA T^CATGA
Sau3AI 1078 GATC ^GATC
Sau96I 146 GGACC G^G[ACGT]CC
SmlI 229 CTCAAG C^T[CT][GA]AG
SphI 1711 GCATGC GCATG^C
StuI 657 AGGCCT AGG^CCT
Tru9I 680 TTAA T^TAA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwNI 8 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
124 CAGTACCTG
BbvI 201 GCAGC GCAGC(8/12)
1422 GCTGC
BsaI 172 GAGACC GGTCTC(1/5)
861 GAGACC
BsmI 247 GAATGC GAATGC(1/-1)
1039 GAATGC
BsrI 1021 ACTGG ACTGG(1/-1)
1777 ACTGG
BstNI 111 CCAGG CC^[AT]GG
601 CCTGG
EcoRII 111 CCAGG ^CC[AT]GG
601 CCTGG
EcoT14I 1332 CCTTGG C^C[AT][AT]GG
1875 CCATGG
Fnu4HI 201 GCAGC GC^[ACGT]GC
1422 GCTGC
HaeIII 658 GGCC GG^CC
1885 GGCC
ItaI 201 GCAGC GC^[ACGT]GC
1422 GCTGC
MvaI 111 CCAGG CC^[AT]GG
601 CCTGG
NspI 712 GCATGT [GA]CATG^[CT]
1711 GCATGC
PleI 28 GACTC GAGTC(4/5)
930 GACTC
SfcI 177 CTACAG C^T[GA][CT]AG
533 CTGCAG
StyI 1332 CCTTGG C^C[AT][AT]GG
1875 CCATGG
TfiI 481 GAATC G^A[AT]TC
1057 GAATC
TseI 201 GCAGC G^C[AT]GC
1422 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AluI Asp700I BpmI BsaJI
BsiYI BslI BsmAI Bsp1407I BsrGI
BssKI BstF5I DdeI FokI HincII
HindII HindIII HinfI HphI MaeIII
MboII MnlI NlaIII RsaI ScrFI
SfaNI SspBI Tsp45I Tsp509I TspRI
XmnI