Restriction map for
pF1KB9127
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
AlwI Aor51HI ApaI AscI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BlnI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmAI
BsmBI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Cac8I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT14I
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI HpaII KasI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AlwNI 1092 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1884 CCTCAGG CC^T[ACGT]AGG
AseI 178 ATTAAT AT^TAAT
AsnI 178 ATTAAT AT^TAAT
BanII 276 GAGCTC G[GA]GC[CT]^C
BfaI 2431 CTAG C^TAG
BglI 1882 GCCCTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 278 GCTCAGC GC^T[ACGT]AGC
BpmI 311 CTGGAG CTGGAG(16/14)
Bpu1102I 278 GCTCAGC GC^T[ACGT]AGC
BsmFI 94 GGGAC GGGAC(10/14)
BssKI 571 CCTGG ^CC[ACGT]GG
BstNI 571 CCTGG CC^[AT]GG
Bsu36I 1884 CCTCAGG CC^T[ACGT]AGG
CelII 278 GCTCAGC GC^T[ACGT]AGC
Cfr13I 491 GGCCC G^G[ACGT]CC
DraII 490 AGGCCCC [GA]G^G[ACGT]CC[CT]
Eco81I 1884 CCTCAGG CC^T[ACGT]AGG
EcoO109I 490 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRII 571 CCTGG ^CC[AT]GG
EcoT22I 2410 ATGCAT ATGCA^T
FauI 2276 GCGGG CCCGC(4/6)
HaeIII 491 GGCC GG^CC
HinfI 587 GATTC G^A[ACGT]TC
MaeI 2431 CTAG C^TAG
MspA1I 116 CCGCTG C[AC]G^C[GT]G
MvaI 571 CCTGG CC^[AT]GG
NdeI 2048 CATATG CA^TATG
NlaIV 491 GGCCCC GG[ACGT]^[ACGT]CC
NsiI 2410 ATGCAT ATGCA^T
NspI 2434 GCATGT [GA]CATG^[CT]
PshBI 178 ATTAAT AT^TAAT
SacI 276 GAGCTC GAGCT^C
Sau96I 491 GGCCC G^G[ACGT]CC
ScrFI 571 CCTGG CC^[ACGT]GG
SmlI 1213 CTCAAG C^T[CT][GA]AG
TfiI 587 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AciI 116 CCGC CCGC(-3/-1)
2276 GCGG
AclI 1863 AACGTT AA^CGTT
2199 AACGTT
BbvI 1711 GCAGC GCAGC(8/12)
2383 GCAGC
BglII 703 AGATCT A^GATCT
1273 AGATCT
Bsp1407I 340 TGTACA T^GTACA
966 TGTACA
BsrGI 340 TGTACA T^GTACA
966 TGTACA
BsrI 461 ACTGG ACTGG(1/-1)
1825 ACTGG
BstYI 703 AGATCT [GA]^GATC[CT]
1273 AGATCT
Fnu4HI 1711 GCAGC GC^[ACGT]GC
2383 GCAGC
HindIII 620 AAGCTT A^AGCTT
2282 AAGCTT
ItaI 1711 GCAGC GC^[ACGT]GC
2383 GCAGC
MflI 703 AGATCT [GA]^GATC[CT]
1273 AGATCT
Psp1406I 1863 AACGTT AA^CGTT
2199 AACGTT
SspBI 340 TGTACA T^GTACA
966 TGTACA
TseI 1711 GCAGC G^C[AT]GC
2383 GCAGC
XhoII 703 AGATCT [GA]^GATC[CT]
1273 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AcsI AfaI AluI Alw44I ApaLI
ApoI Asp700I AspHI BbsI BmyI
BpuAI BsiHKAI BsiYI BslI BsmI
Bsp1286I BstF5I DdeI DpnII EcoRI
FokI HphI MaeII MaeIII MboI
MboII MnlI MseI MslI MwoI
NdeII NlaIII RsaI Sau3AI SfaNI
Tru9I Tsp45I Tsp509I TspRI XmnI