Restriction map for
pF1KB5623
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII AccI AciI AcyI AgeI
AhdI AocI Aor51HI ApaI AscI
AspEI AspI AvaI AviII BbeI
BbsI BbvCI BcgI BclI BglI
BglII BlpI Bpu1102I BpuAI BsaHI
BsgI BsiWI BsmBI BsoBI BspDI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII Hin1I
HindIII HpaI KasI KspI MfeI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PvuI RsrII SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1976 GGTACC G^GTACC
AccIII 2199 TCCGGA T^CCGGA
AclI 83 AACGTT AA^CGTT
Alw44I 1063 GTGCAC G^TGCAC
ApaLI 1063 GTGCAC G^TGCAC
AseI 2352 ATTAAT AT^TAAT
AsnI 2352 ATTAAT AT^TAAT
Asp700I 1077 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1976 GGTACC G^GTACC
AvaII 1732 GGTCC G^G[AT]CC
AvrII 2460 CCTAGG C^CTAGG
BalI 2613 TGGCCA TGG^CCA
BamHI 771 GGATCC G^GATCC
BbrPI 1888 CACGTG CAC^GTG
BcnI 2079 CCCGG CC^[GC]GG
BlnI 2460 CCTAGG C^CTAGG
BsaAI 1888 CACGTG [CT]AC^GT[GA]
BsaI 2302 GAGACC GGTCTC(1/5)
BsaWI 2199 TCCGGA [AT]^CCGG[AT]
BseAI 2199 TCCGGA T^CCGGA
BsiEI 2879 CGACCG CG[GA][CT]^CG
BsmI 589 GCATTC GAATGC(1/-1)
BspEI 2199 TCCGGA T^CCGGA
BspHI 1049 TCATGA T^CATGA
BspLU11I 579 ACATGT A^CATGT
BspMI 1185 ACCTGC ACCTGC(4/8)
BsrDI 41 CATTGC GCAATG(2/0)
BstXI 376 CCAAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1204 GCGC GCG^C
EaeI 2613 TGGCCA [CT]^GGCC[GA]
EarI 951 GAAGAG CTCTTC(1/4)
HhaI 1204 GCGC GCG^C
HinP1I 1204 GCGC G^CGC
KpnI 1976 GGTACC GGTAC^C
Ksp632I 951 GAAGAG CTCTTC(1/4)
MluI 130 ACGCGT A^CGCGT
MluNI 2613 TGGCCA TGG^CCA
MroI 2199 TCCGGA T^CCGGA
MscI 2613 TGGCCA TGG^CCA
MspA1I 2726 CAGCTG C[AC]G^C[GT]G
NciI 2079 CCCGG CC^[GC]GG
PmaCI 1888 CACGTG CAC^GTG
PmlI 1888 CACGTG CAC^GTG
PpuMI 1731 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 2352 ATTAAT AT^TAAT
Psp1406I 83 AACGTT AA^CGTT
PstI 1568 CTGCAG CTGCA^G
PvuII 2726 CAGCTG CAG^CTG
RcaI 1049 TCATGA T^CATGA
SfcI 1568 CTGCAG C^T[GA][CT]AG
SpeI 2152 ACTAGT A^CTAGT
SphI 1377 GCATGC GCATG^C
XmnI 1077 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccII 131 CGCG CG^CG
1205 CGCG
AflII 945 CTTAAG C^TTAAG
1170 CTTAAG
AflIII 130 ACGCGT A^C[GA][CT]GT
579 ACATGT
BfrI 945 CTTAAG C^TTAAG
1170 CTTAAG
BsaBI 204 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2223 GATTCATATC
Bsp1407I 417 TGTACA T^GTACA
862 TGTACA
BsrGI 417 TGTACA T^GTACA
862 TGTACA
BstUI 131 CGCG CG^CG
1205 CGCG
DsaI 1110 CCATGG C^C[GA][CT]GG
2240 CCATGG
HapII 2080 CCGG C^CGG
2200 CCGG
HincII 1015 GTCAAC GT[CT]^[GA]AC
2493 GTTGAC
HindII 1015 GTCAAC GT[CT]^[GA]AC
2493 GTTGAC
HpaII 2080 CCGG C^CGG
2200 CCGG
MamI 204 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2223 GATTCATATC
MspI 2080 CCGG C^CGG
2200 CCGG
MvnI 131 CGCG CG^CG
1205 CGCG
NcoI 1110 CCATGG C^CATGG
2240 CCATGG
NspI 579 ACATGT [GA]CATG^[CT]
1377 GCATGC
SacI 304 GAGCTC GAGCT^C
1444 GAGCTC
ScaI 705 AGTACT AGT^ACT
1163 AGTACT
SexAI 275 ACCAGGT A^CC[AT]GGT
1959 ACCTGGT
SspBI 417 TGTACA T^GTACA
862 TGTACA
XcmI 1190 CCACAGAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1523 CCAACAACAGTGTGG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
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AcsI AfaI AluI AlwI AlwNI
ApoI AspHI BanI BanII BbvI
BciVI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HgaI HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII