Restriction map for
pF1KB3487
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI Alw44I ApaI ApaLI AscI
AseI AsnI Asp718I AspI AviII
AvrII BamHI BbvCI BfrI BglII
BlnI BsaBI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Cfr10I ClaI CpoI
DraI DraIII EagI EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 1180 GACGTC GACGT^C
AccI 1090 GTCTAC GT^[AC][GT]AC
AcsI 181 GAATTT [GA]^AATT[CT]
AhdI 1006 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1263 CCTGAGG CC^T[ACGT]AGG
Aor51HI 34 AGCGCT AGC^GCT
ApoI 181 GAATTT [GA]^AATT[CT]
Asp700I 1647 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1006 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1858 GAGCAC G[AT]GC[AT]^C
BanII 384 GGGCTC G[GA]GC[CT]^C
BbeI 41 GGCGCC GGCGC^C
BbrPI 1270 CACGTG CAC^GTG
BcgI 552 GCAGGCCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 162 GTATCC GTATCC(6/5)
BclI 836 TGATCA T^GATCA
BglI 1136 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 30 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 30 GCTGAGC GC^T[ACGT]AGC
BsaAI 1270 CACGTG [CT]AC^GT[GA]
BsiHKAI 1858 GAGCAC G[AT]GC[AT]^C
BssHII 1769 GCGCGC G^CGCGC
Bsu36I 1263 CCTGAGG CC^T[ACGT]AGG
CelII 30 GCTGAGC GC^T[ACGT]AGC
DrdI 1375 GACCTGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1006 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 34 AGCGCT AGC^GCT
Eco81I 1263 CCTGAGG CC^T[ACGT]AGG
EcoNI 237 CCTTTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 836 TGATCA T^GATCA
HgaI 1012 GCGTC GACGC(5/10)
HphI 839 TCACC GGTGA(8/7)
KasI 41 GGCGCC G^GCGCC
NarI 41 GGCGCC GG^CGCC
NruI 998 TCGCGA TCG^CGA
PmaCI 1270 CACGTG CAC^GTG
PmlI 1270 CACGTG CAC^GTG
SfoI 41 GGCGCC GGC^GCC
SmlI 532 CTCAAG C^T[CT][GA]AG
XbaI 1015 TCTAGA T^CTAGA
XmnI 1647 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 1258 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1705 CAGAACCTG
AvaI 89 CCCGAG C^[CT]CG[GA]G
1354 CTCGGG
BanI 41 GGCGCC G^G[CT][GA]CC
1342 GGTGCC
BbsI 1647 GAAGAC GAAGAC(2/6)
1783 GAAGAC
BpuAI 1647 GAAGAC GAAGAC(2/6)
1783 GAAGAC
BseRI 343 GAGGAG GAGGAG(10/8)
1855 GAGGAG
BsgI 795 CTGCAC GTGCAG(16/14)
1102 CTGCAC
BsiEI 1033 CGGTCG CG[GA][CT]^CG
1656 CGACCG
BsmI 947 GCATTC GAATGC(1/-1)
1235 GCATTC
BsoBI 89 CCCGAG C^[CT]CG[GA]G
1354 CTCGGG
BspHI 177 TCATGA T^CATGA
1478 TCATGA
BspMI 1094 ACCTGC ACCTGC(4/8)
1257 GCAGGT
BstYI 666 GGATCT [GA]^GATC[CT]
1049 AGATCC
DsaI 446 CCGTGG C^C[GA][CT]GG
693 CCGTGG
EarI 1061 GAAGAG CTCTTC(1/4)
1414 GAAGAG
EcoT14I 56 CCAAGG C^C[AT][AT]GG
662 CCTTGG
Ksp632I 1061 GAAGAG CTCTTC(1/4)
1414 GAAGAG
MflI 666 GGATCT [GA]^GATC[CT]
1049 AGATCC
MslI 828 CACGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1270 CACGTGAATG
PflMI 56 CCAAGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1456 CCAAACGCTGG
RcaI 177 TCATGA T^CATGA
1478 TCATGA
StuI 849 AGGCCT AGG^CCT
1426 AGGCCT
StyI 56 CCAAGG C^C[AT][AT]GG
662 CCTTGG
Van91I 56 CCAAGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1456 CCAAACGCTGG
XcmI 638 CCAGCTATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
807 CCACACCAGCACTGG
XhoII 666 GGATCT [GA]^GATC[CT]
1049 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaII BalI BbvI BcnI
BfaI BmyI BpmI BsaHI BsaI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII ItaI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SphI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I