Restriction map for
pF1KB3044
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccIII AgeI Alw44I
AlwNI AocI ApaI ApaLI AscI
Asp718I AvaI AviII AvrII BalI
BbeI BbrPI BbvCI BcgI BclI
BcnI BglI BglII BlnI BlpI
Bpu1102I BsaI BsaWI BseAI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstEII BstPI BstXI
Bsu36I CelII Cfr10I ClaI CpoI
DrdI DsaI EaeI EagI EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HapII HindIII HpaI HpaII KasI
KpnI KspI MluNI MroI MscI
MspI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacI SacII SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccII 629 CGCG CG^CG
AclI 1820 AACGTT AA^CGTT
AcsI 1810 AAATTT [GA]^AATT[CT]
AcyI 1354 GGCGTC G[GA]^CG[CT]C
AflII 892 CTTAAG C^TTAAG
AhdI 1364 GACATGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 74 AGCGCT AGC^GCT
ApoI 1810 AAATTT [GA]^AATT[CT]
Asp700I 1426 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1364 GACATGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 51 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1764 GGCACC G^G[CT][GA]CC
BanII 30 GGGCTC G[GA]GC[CT]^C
BbsI 1134 GTCTTC GAAGAC(2/6)
BciVI 1803 GTATCC GTATCC(6/5)
BfaI 1147 CTAG C^TAG
BfrI 892 CTTAAG C^TTAAG
BpuAI 1134 GTCTTC GAAGAC(2/6)
BsaAI 470 TACGTG [CT]AC^GT[GA]
BsaHI 1354 GGCGTC G[GA]^CG[CT]C
BsgI 1201 GTGCAG GTGCAG(16/14)
BspHI 1602 TCATGA T^CATGA
BspLU11I 687 ACATGT A^CATGT
Bst1107I 1285 GTATAC GTA^TAC
BstAPI 748 GCAACTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1535 TTCGAA TT^CGAA
BstUI 629 CGCG CG^CG
BstZ17I 1285 GTATAC GTA^TAC
CfoI 75 GCGC GCG^C
DraIII 1019 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1364 GACATGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 74 AGCGCT AGC^GCT
EcoT14I 1670 CCTTGG C^C[AT][AT]GG
FauI 80 GCGGG CCCGC(4/6)
HaeII 74 AGCGCT [GA]GCGC^[CT]
HhaI 75 GCGC GCG^C
Hin1I 1354 GGCGTC G[GA]^CG[CT]C
HinP1I 75 GCGC G^CGC
MaeI 1147 CTAG C^TAG
MfeI 1248 CAATTG C^AATTG
MluI 628 ACGCGT A^CGCGT
MslI 1603 CATGACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 813 CAGCTG C[AC]G^C[GT]G
MunI 1248 CAATTG C^AATTG
MvnI 629 CGCG CG^CG
NdeI 1733 CATATG CA^TATG
NspI 687 ACATGT [GA]CATG^[CT]
NspV 1535 TTCGAA TT^CGAA
PflFI 51 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 55 GAGTC GAGTC(4/5)
PpuMI 83 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 1820 AACGTT AA^CGTT
PvuII 813 CAGCTG CAG^CTG
RcaI 1602 TCATGA T^CATGA
SalI 383 GTCGAC G^TCGAC
SapI 1121 GCTCTTC GCTCTTC(1/4)
ScaI 539 AGTACT AGT^ACT
SfuI 1535 TTCGAA TT^CGAA
SmlI 892 CTTAAG C^T[CT][GA]AG
StyI 1670 CCTTGG C^C[AT][AT]GG
Tth111I 51 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1247 CCAATTGGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1426 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 540 GTAC GT^AC
656 GTAC
AflIII 628 ACGCGT A^C[GA][CT]GT
687 ACATGT
AseI 706 ATTAAT AT^TAAT
949 ATTAAT
AsnI 706 ATTAAT AT^TAAT
949 ATTAAT
AspHI 293 GTGCTC G[AT]GC[AT]^C
1610 GTGCTC
AvaII 84 GGTCC G^G[AT]CC
916 GGACC
BseRI 1613 CTCCTC GAGGAG(10/8)
37 GAGGAG
BsiHKAI 293 GTGCTC G[AT]GC[AT]^C
1610 GTGCTC
BsiYI 1680 CCCACCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1681 CCACCAGAGGG
BslI 1680 CCCACCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1681 CCACCAGAGGG
BsmBI 49 GAGACG CGTCTC(1/5)
1511 GAGACG
BsrI 828 ACTGG ACTGG(1/-1)
1409 ACTGG
DraII 83 GGGTCCT [GA]G^G[ACGT]CC[CT]
933 AGGCCCT
EcoO109I 83 GGGTCCT [GA]G^G[ACGT]CC[CT]
933 AGGCCCT
HgaI 381 GCGTC GACGC(5/10)
1355 GCGTC
HphI 640 GGTGA GGTGA(8/7)
1032 GGTGA
PshBI 706 ATTAAT AT^TAAT
949 ATTAAT
PstI 192 CTGCAG CTGCA^G
1850 CTGCAG
RsaI 540 GTAC GT^AC
656 GTAC
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccI AciI AluI AlwI BamHI
BbvI BmyI BpmI BsaBI BsaJI
BsmAI BsmFI Bsp1286I BsrDI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRII
Fnu4HI FokI HaeIII HincII HindII
HinfI ItaI Ksp632I MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII