Restriction map for
pF1KE0100
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# NO CUT ENZYMES ( 107 enzymes) #
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AatII Acc65I AccIII AflII AgeI
AhdI Alw44I AocI ApaLI AscI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BbvCI BcgI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI DraII DrdI
Eam1105I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI MamI MluI MluNI MroI
MscI NaeI NgoMIV NheI NotI
NruI NsiI NspI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PvuI RcaI SacI SalI SbfI
ScaI SexAI SfiI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AclI 1558 AACGTT AA^CGTT
AcyI 148 GGCGCC G[GA]^CG[CT]C
AflIII 2345 ACGTGT A^C[GA][CT]GT
AlwNI 170 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 2230 AGCGCT AGC^GCT
ApaI 61 GGGCCC GGGCC^C
BamHI 1958 GGATCC G^GATCC
BbeI 148 GGCGCC GGCGC^C
BbrPI 1489 CACGTG CAC^GTG
BsaHI 148 GGCGCC G[GA]^CG[CT]C
BsgI 685 GTGCAG GTGCAG(16/14)
BsiEI 188 CGGCCG CG[GA][CT]^CG
BsmFI 1174 GGGAC GGGAC(10/14)
BsrBI 251 CCGCTC CCGCTC(-3/-3)
BsrI 270 ACTGG ACTGG(1/-1)
BstAPI 169 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2042 TTCGAA TT^CGAA
CpoI 156 CGGTCCG CG^G[AT]CCG
DraI 2071 TTTAAA TTT^AAA
DraIII 846 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 188 CGGCCG C^GGCCG
EclXI 188 CGGCCG C^GGCCG
Eco47III 2230 AGCGCT AGC^GCT
Eco52I 188 CGGCCG C^GGCCG
EcoNI 2205 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 2300 CCATGG C^C[AT][AT]GG
Hin1I 148 GGCGCC G[GA]^CG[CT]C
KasI 148 GGCGCC G^GCGCC
KspI 256 CCGCGG CCGC^GG
NarI 148 GGCGCC GG^CGCC
NcoI 2300 CCATGG C^CATGG
NdeI 1820 CATATG CA^TATG
NspV 2042 TTCGAA TT^CGAA
PacI 1642 TTAATTAA TTAAT^TAA
PmaCI 1489 CACGTG CAC^GTG
PmlI 1489 CACGTG CAC^GTG
Psp1406I 1558 AACGTT AA^CGTT
PvuII 1573 CAGCTG CAG^CTG
RsrII 156 CGGTCCG CG^G[AT]CCG
SacII 256 CCGCGG CCGC^GG
SfoI 148 GGCGCC GGC^GCC
SfuI 2042 TTCGAA TT^CGAA
SmaI 64 CCCGGG CCC^GGG
StyI 2300 CCATGG C^C[AT][AT]GG
Tsp45I 701 GTCAC ^GT[GC]AC
XmaCI 64 CCCGGG C^CCGGG
XmaI 64 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 890 GTCTAC GT^[AC][GT]AC
1374 GTCTAC
AccII 102 CGCG CG^CG
257 CGCG
AseI 1356 ATTAAT AT^TAAT
2484 ATTAAT
AsnI 1356 ATTAAT AT^TAAT
2484 ATTAAT
BanI 148 GGCGCC G^G[CT][GA]CC
783 GGCACC
BciVI 829 GGATAC GTATCC(6/5)
1843 GGATAC
BglI 295 GCCTGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1130 GCCAGCTAGGC
BsaAI 1489 CACGTG [CT]AC^GT[GA]
2344 TACGTG
BsaI 1411 GAGACC GGTCTC(1/5)
2168 GAGACC
BseRI 307 GAGGAG GAGGAG(10/8)
1312 GAGGAG
BsmI 1583 GAATGC GAATGC(1/-1)
2105 GCATTC
BsrDI 929 CATTGC GCAATG(2/0)
1396 GCAATG
BstUI 102 CGCG CG^CG
257 CGCG
MfeI 1838 CAATTG C^AATTG
2391 CAATTG
MunI 1838 CAATTG C^AATTG
2391 CAATTG
MvnI 102 CGCG CG^CG
257 CGCG
PshBI 1356 ATTAAT AT^TAAT
2484 ATTAAT
SapI 1462 GAAGAGC GCTCTTC(1/4)
2217 GAAGAGC
TaqI 2043 TCGA T^CGA
2085 TCGA
TspRI 170 CAGTG CA[GC]TG[ACGT][ACGT]^
948 CACTG
TthHB8I 2043 TCGA T^CGA
2085 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI BsoBI Bsp1286I BspMI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TfiI Tru9I TseI
Tsp509I XhoII XmnI