Restriction map for
pF1KA0726
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# NO CUT ENZYMES ( 96 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BbeI BbsI BclI BfrI
BglI BlnI BpuAI BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
EcoRV FbaI FseI HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MseI MunI NarI
NdeI NheI NotI NruI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tth111I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AhdI 1543 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2277 CCTGAGG CC^T[ACGT]AGG
AspEI 1543 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2603 TGCGCA TGC^GCA
BalI 872 TGGCCA TGG^CCA
BamHI 2151 GGATCC G^GATCC
BbvCI 694 GCTGAGG CCTCAGC(-5/-2)
BciVI 800 GTATCC GTATCC(6/5)
BfaI 2503 CTAG C^TAG
BlpI 2439 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2439 GCTCAGC GC^T[ACGT]AGC
BseRI 1486 GAGGAG GAGGAG(10/8)
BsiEI 480 CGATCG CG[GA][CT]^CG
BspHI 1463 TCATGA T^CATGA
BsrDI 2372 GCAATG GCAATG(2/0)
BsrFI 2638 GCCGGC [GA]^CCGG[CT]
BstEII 2467 GGTCACC G^GT[ACGT]ACC
BstPI 2467 GGTCACC G^GT[ACGT]ACC
Bsu36I 2277 CCTGAGG CC^T[ACGT]AGG
CelII 2439 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2638 GCCGGC [GA]^CCGG[CT]
DrdI 121 GACAACACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1543 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2277 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2467 GGTCACC G^GT[ACGT]ACC
EcoRI 2377 GAATTC G^AATTC
EcoT22I 1640 ATGCAT ATGCA^T
FspI 2603 TGCGCA TGC^GCA
HincII 403 GTCAAC GT[CT]^[GA]AC
HindII 403 GTCAAC GT[CT]^[GA]AC
MaeI 2503 CTAG C^TAG
MluNI 872 TGGCCA TGG^CCA
MscI 872 TGGCCA TGG^CCA
NaeI 2638 GCCGGC GCC^GGC
NgoMIV 2638 GCCGGC G^CCGGC
NsiI 1640 ATGCAT ATGCA^T
PaeR7I 1360 CTCGAG C^TCGAG
PflMI 769 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2672 AGGACCC [GA]G^G[AT]CC[CT]
PvuI 480 CGATCG CGAT^CG
RcaI 1463 TCATGA T^CATGA
SacI 2209 GAGCTC GAGCT^C
StuI 329 AGGCCT AGG^CCT
Van91I 769 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1360 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 2126 AAATTT [GA]^AATT[CT]
2377 GAATTC
Alw44I 1000 GTGCAC G^TGCAC
2104 GTGCAC
ApaI 348 GGGCCC GGGCC^C
1093 GGGCCC
ApaLI 1000 GTGCAC G^TGCAC
2104 GTGCAC
ApoI 2126 AAATTT [GA]^AATT[CT]
2377 GAATTC
AvaI 1360 CTCGAG C^[CT]CG[GA]G
2155 CCCGAG
BbrPI 1702 CACGTG CAC^GTG
2434 CACGTG
BcgI 988 GCAGGACTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1394 GCATCTCCTTCG
BglII 191 AGATCT A^GATCT
536 AGATCT
BsoBI 1360 CTCGAG C^[CT]CG[GA]G
2155 CCCGAG
BsrBI 904 GAGCGG CCGCTC(-3/-3)
2158 GAGCGG
DraIII 767 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2032 CACCAGGTG
EcoNI 243 CCTTGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2277 CCTGAGGCAGG
HaeII 909 GGCGCT [GA]GCGC^[CT]
2536 AGCGCC
PmaCI 1702 CACGTG CAC^GTG
2434 CACGTG
PmlI 1702 CACGTG CAC^GTG
2434 CACGTG
PvuII 732 CAGCTG CAG^CTG
1961 CAGCTG
SexAI 768 ACCAGGT A^CC[AT]GGT
2033 ACCAGGT
SmlI 1279 CTTGAG C^T[CT][GA]AG
1360 CTCGAG
Tsp509I 2127 AATT ^AATT
2378 AATT
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaAI BsaHI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XcmI XhoII