Restriction map for
pF1KA0382
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I ApaI ApaLI
Asp718I AspEI AvaI AviII AvrII
BamHI BbeI BbrPI BcgI BfrI
BlnI BlpI Bpu1102I BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspEI BsrFI BsrGI BssSI
Bst1107I BstEII BstPI BstZ17I CelII
Cfr10I CpoI DraI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV EcoT22I
FseI FspI KasI KpnI KspI
MluI MroI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 15 GTCTAC GT^[AC][GT]AC
AccII 1871 CGCG CG^CG
AcyI 437 GACGCC G[GA]^CG[CT]C
Aor51HI 4433 AGCGCT AGC^GCT
AscI 1869 GGCGCGCC GG^CGCGCC
AseI 2374 ATTAAT AT^TAAT
AsnI 2374 ATTAAT AT^TAAT
BalI 2857 TGGCCA TGG^CCA
BanI 4029 GGCACC G^G[CT][GA]CC
BbvCI 739 CCTCAGC CCTCAGC(-5/-2)
BcnI 1143 CCCGG CC^[GC]GG
BglI 307 GCCATGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3614 TACGTG [CT]AC^GT[GA]
BsaHI 437 GACGCC G[GA]^CG[CT]C
BspLU11I 1549 ACATGT A^CATGT
BsrBI 1543 GAGCGG CCGCTC(-3/-3)
BssHII 1870 GCGCGC G^CGCGC
BstAPI 3745 GCATTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1871 CGCG CG^CG
DraIII 4160 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2857 TGGCCA [CT]^GGCC[GA]
Eco47III 4433 AGCGCT AGC^GCT
EcoNI 3445 CCTGGCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2462 GAATTC G^AATTC
FauI 312 GCGGG CCCGC(4/6)
HapII 1144 CCGG C^CGG
Hin1I 437 GACGCC G[GA]^CG[CT]C
HindIII 3284 AAGCTT A^AGCTT
HpaI 4198 GTTAAC GTT^AAC
HpaII 1144 CCGG C^CGG
MfeI 1841 CAATTG C^AATTG
MluNI 2857 TGGCCA TGG^CCA
MscI 2857 TGGCCA TGG^CCA
MspI 1144 CCGG C^CGG
MunI 1841 CAATTG C^AATTG
MvnI 1871 CGCG CG^CG
NciI 1143 CCCGG CC^[GC]GG
PshAI 500 GACATATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2374 ATTAAT AT^TAAT
SmlI 1267 CTTGAG C^T[CT][GA]AG
SpeI 565 ACTAGT A^CTAGT
StuI 3911 AGGCCT AGG^CCT
XcmI 4584 CCAAAGGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AocI 1369 CCTGAGG CC^T[ACGT]AGG
1918 CCTCAGG
AspI 159 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3880 GACAGTGTC
BciVI 1975 GGATAC GTATCC(6/5)
2449 GTATCC
BclI 2427 TGATCA T^GATCA
2690 TGATCA
BglII 1338 AGATCT A^GATCT
2906 AGATCT
BsaI 1815 GAGACC GGTCTC(1/5)
2569 GAGACC
BspDI 1292 ATCGAT AT^CGAT
2581 ATCGAT
BsrDI 114 CATTGC GCAATG(2/0)
3963 CATTGC
Bsu36I 1369 CCTGAGG CC^T[ACGT]AGG
1918 CCTCAGG
ClaI 1292 ATCGAT AT^CGAT
2581 ATCGAT
DrdI 265 GACAATCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1817 GACCAAGCCGTC
Eco81I 1369 CCTGAGG CC^T[ACGT]AGG
1918 CCTCAGG
FbaI 2427 TGATCA T^GATCA
2690 TGATCA
HincII 3438 GTCAAC GT[CT]^[GA]AC
4198 GTTAAC
HindII 3438 GTCAAC GT[CT]^[GA]AC
4198 GTTAAC
NcoI 1853 CCATGG C^CATGG
4100 CCATGG
NdeI 502 CATATG CA^TATG
3934 CATATG
NspI 1549 ACATGT [GA]CATG^[CT]
2643 ACATGC
PflFI 159 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3880 GACAGTGTC
PflMI 4503 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4584 CCAAAGGCTGG
PpuMI 1803 GGGACCC [GA]G^G[AT]CC[CT]
3371 AGGACCT
SapI 3022 GAAGAGC GCTCTTC(1/4)
3813 GAAGAGC
SspI 2836 AATATT AAT^ATT
3904 AATATT
Tth111I 159 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3880 GACAGTGTC
Van91I 4503 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4584 CCAAAGGCTGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaBI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspMI
BsrI BssKI BstBI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HgaI HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspV PleI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII XmnI