Restriction map for
pF1KA0129
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII AccI AccIII AclI AflIII
AgeI AhdI AocI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BbvCI BcgI BciVI BclI
BfaI BglII BlnI BsaBI BsaI
BsaWI BseAI BseRI BsiWI BsmAI
BsmBI BsmFI BsmI BspDI BspEI
BspLU11I BsrDI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DrdI Eam1105I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HindIII HpaI
MaeI MamI MfeI MluI MroI
MslI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
RsrII SalI SbfI SexAI SfcI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TfiI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 742 GGTACC G^GTACC
AflII 691 CTTAAG C^TTAAG
Alw44I 196 GTGCAC G^TGCAC
AlwNI 489 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 892 GGGCCC GGGCC^C
ApaLI 196 GTGCAC G^TGCAC
Asp718I 742 GGTACC G^GTACC
AvaI 580 CTCGGG C^[CT]CG[GA]G
AvaII 26 GGACC G^G[AT]CC
BamHI 813 GGATCC G^GATCC
BbrPI 199 CACGTG CAC^GTG
BbsI 1177 GTCTTC GAAGAC(2/6)
BfrI 691 CTTAAG C^TTAAG
BglI 875 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 94 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 94 GCTGAGC GC^T[ACGT]AGC
BpuAI 1177 GTCTTC GAAGAC(2/6)
BsaAI 199 CACGTG [CT]AC^GT[GA]
BsoBI 580 CTCGGG C^[CT]CG[GA]G
Bsp1407I 428 TGTACA T^GTACA
BspHI 470 TCATGA T^CATGA
BspMI 1238 ACCTGC ACCTGC(4/8)
BsrBI 1270 CCGCTC CCGCTC(-3/-3)
BsrGI 428 TGTACA T^GTACA
BstYI 813 GGATCC [GA]^GATC[CT]
CelII 94 GCTGAGC GC^T[ACGT]AGC
DraI 225 TTTAAA TTT^AAA
DraIII 166 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
HincII 976 GTTGAC GT[CT]^[GA]AC
HindII 976 GTTGAC GT[CT]^[GA]AC
HphI 766 TCACC GGTGA(8/7)
KpnI 742 GGTACC GGTAC^C
KspI 1038 CCGCGG CCGC^GG
MflI 813 GGATCC [GA]^GATC[CT]
PflMI 165 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 199 CACGTG CAC^GTG
PmlI 199 CACGTG CAC^GTG
PvuI 852 CGATCG CGAT^CG
PvuII 232 CAGCTG CAG^CTG
RcaI 470 TCATGA T^CATGA
SacI 97 GAGCTC GAGCT^C
SacII 1038 CCGCGG CCGC^GG
SapI 99 GCTCTTC GCTCTTC(1/4)
ScaI 1109 AGTACT AGT^ACT
SspBI 428 TGTACA T^GTACA
TspRI 345 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 165 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 813 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcsI 340 AAATTC [GA]^AATT[CT]
391 AAATTC
Aor51HI 378 AGCGCT AGC^GCT
1094 AGCGCT
ApoI 340 AAATTC [GA]^AATT[CT]
391 AAATTC
AspHI 97 GAGCTC G[AT]GC[AT]^C
196 GTGCAC
BalI 162 TGGCCA TGG^CCA
383 TGGCCA
BbeI 994 GGCGCC GGCGC^C
1034 GGCGCC
BsgI 202 GTGCAG GTGCAG(16/14)
982 GTGCAG
BsiHKAI 97 GAGCTC G[AT]GC[AT]^C
196 GTGCAC
BssSI 643 CTCGTG CACGAG(-5/-1)
938 CTCGTG
BstAPI 555 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
701 GCATAAACTGC
EagI 957 CGGCCG C^GGCCG
1049 CGGCCG
EarI 100 CTCTTC CTCTTC(1/4)
370 GAAGAG
EclXI 957 CGGCCG C^GGCCG
1049 CGGCCG
Eco47III 378 AGCGCT AGC^GCT
1094 AGCGCT
Eco52I 957 CGGCCG C^GGCCG
1049 CGGCCG
HinfI 313 GAGTC G^A[ACGT]TC
445 GACTC
KasI 994 GGCGCC G^GCGCC
1034 GGCGCC
Ksp632I 100 CTCTTC CTCTTC(1/4)
370 GAAGAG
MaeIII 941 GTGAC ^GT[ACGT]AC
1219 GTGAC
MluNI 162 TGGCCA TGG^CCA
383 TGGCCA
MscI 162 TGGCCA TGG^CCA
383 TGGCCA
NaeI 997 GCCGGC GCC^GGC
1195 GCCGGC
NarI 994 GGCGCC GG^CGCC
1034 GGCGCC
NgoMIV 997 GCCGGC G^CCGGC
1195 GCCGGC
PleI 445 GACTC GAGTC(4/5)
313 GAGTC
SfoI 994 GGCGCC GGC^GCC
1034 GGCGCC
Tsp45I 941 GTGAC ^GT[GC]AC
1219 GTGAC
Tsp509I 341 AATT ^AATT
392 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI BanI BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BsiEI
BsiYI BslI Bsp1286I BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII ItaI MaeII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TaqI Tru9I
TseI TthHB8I