Restriction map for
pF1KB5210
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MseI MunI NarI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 489 CGCG CG^CG
AcyI 238 GGCGTC G[GA]^CG[CT]C
Alw44I 170 GTGCAC G^TGCAC
ApaLI 170 GTGCAC G^TGCAC
AspHI 170 GTGCAC G[AT]GC[AT]^C
AvaI 381 CCCGGG C^[CT]CG[GA]G
BalI 356 TGGCCA TGG^CCA
BfaI 367 CTAG C^TAG
BglI 805 GCCTGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 238 GGCGTC G[GA]^CG[CT]C
BseRI 186 CTCCTC GAGGAG(10/8)
BsgI 19 CTGCAC GTGCAG(16/14)
BsiHKAI 170 GTGCAC G[AT]GC[AT]^C
BsoBI 381 CCCGGG C^[CT]CG[GA]G
BspMI 273 ACCTGC ACCTGC(4/8)
BsrFI 67 GCCGGC [GA]^CCGG[CT]
BssSI 892 CTCGTG CACGAG(-5/-1)
BstUI 489 CGCG CG^CG
Cfr10I 67 GCCGGC [GA]^CCGG[CT]
DdeI 689 CTCAG C^T[ACGT]AG
DrdI 559 GACGTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 575 CCATGG C^C[GA][CT]GG
Hin1I 238 GGCGTC G[GA]^CG[CT]C
HinfI 867 GAATC G^A[ACGT]TC
MaeI 367 CTAG C^TAG
MaeIII 41 GTGAC ^GT[ACGT]AC
MluNI 356 TGGCCA TGG^CCA
MscI 356 TGGCCA TGG^CCA
MvnI 489 CGCG CG^CG
NaeI 67 GCCGGC GCC^GGC
NcoI 575 CCATGG C^CATGG
NdeI 218 CATATG CA^TATG
NgoMIV 67 GCCGGC G^CCGGC
PstI 538 CTGCAG CTGCA^G
SmaI 381 CCCGGG CCC^GGG
TaqI 350 TCGA T^CGA
TfiI 867 GAATC G^A[AT]TC
Tsp45I 41 GTGAC ^GT[GC]AC
TthHB8I 350 TCGA T^CGA
XcmI 311 CCACAGTCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 381 CCCGGG C^CCGGG
XmaI 381 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 79 GTCTAC GT^[AC][GT]AC
391 GTCTAC
AlwNI 532 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
691 CAGGCCCTG
AvaII 528 GGTCC G^G[AT]CC
741 GGACC
BanII 686 GGGCTC G[GA]GC[CT]^C
799 GAGCCC
BbrPI 22 CACGTG CAC^GTG
838 CACGTG
BbsI 27 GAAGAC GAAGAC(2/6)
675 GAAGAC
BpmI 8 CTGGAG CTGGAG(16/14)
859 CTGGAG
BpuAI 27 GAAGAC GAAGAC(2/6)
675 GAAGAC
BsaAI 22 CACGTG [CT]AC^GT[GA]
838 CACGTG
BsmFI 557 GGGAC GGGAC(10/14)
241 GTCCC
BsrI 858 ACTGG ACTGG(1/-1)
650 CCAGT
BstXI 248 CCACCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
659 CCAAGGCCCTGG
BstYI 122 AGATCC [GA]^GATC[CT]
788 AGATCC
HaeII 290 AGCGCC [GA]GCGC^[CT]
942 GGCGCT
HgaI 77 GCGTC GACGC(5/10)
239 GCGTC
MflI 122 AGATCC [GA]^GATC[CT]
788 AGATCC
PmaCI 22 CACGTG CAC^GTG
838 CACGTG
PmlI 22 CACGTG CAC^GTG
838 CACGTG
PvuII 934 CAGCTG CAG^CTG
955 CAGCTG
SfcI 538 CTGCAG C^T[GA][CT]AG
831 CTATAG
XhoII 122 AGATCC [GA]^GATC[CT]
788 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwI BanI BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmAI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DpnII DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SmlI StyI TseI TspRI