Restriction map for
pF1KB5139
##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI AocI ApoI
AscI AseI AsnI AspEI AspI
AviII AvrII BamHI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI Eam1105I EarI Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI Ksp632I MamI
MfeI MflI MluI MroI MseI
MunI NheI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI RcaI RsrII SapI
SbfI ScaI SfiI SfuI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I Tth111I
XbaI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1038 GGTACC G^GTACC
AccI 694 GTCGAC GT^[AC][GT]AC
AcyI 1621 GGCGCC G[GA]^CG[CT]C
AfaI 1039 GTAC GT^AC
AlwI 947 GATCC GGATC(4/5)
AlwNI 85 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1227 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1038 GGTACC G^GTACC
BbeI 1621 GGCGCC GGCGC^C
BbsI 1242 GAAGAC GAAGAC(2/6)
BfaI 574 CTAG C^TAG
BglI 511 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1242 GAAGAC GAAGAC(2/6)
BsaAI 595 CACGTA [CT]AC^GT[GA]
BsaHI 1621 GGCGCC G[GA]^CG[CT]C
BsgI 304 CTGCAC GTGCAG(16/14)
BsmBI 453 GAGACG CGTCTC(1/5)
BstAPI 881 GCAAAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 663 GGTGACC G^GT[ACGT]ACC
BstPI 663 GGTGACC G^GT[ACGT]ACC
DraIII 1249 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 663 GGTGACC G^GT[ACGT]ACC
Hin1I 1621 GGCGCC G[GA]^CG[CT]C
HincII 694 GTCGAC GT[CT]^[GA]AC
HindII 694 GTCGAC GT[CT]^[GA]AC
KasI 1621 GGCGCC G^GCGCC
KpnI 1038 GGTACC GGTAC^C
MaeI 574 CTAG C^TAG
NaeI 1000 GCCGGC GCC^GGC
NarI 1621 GGCGCC GG^CGCC
NcoI 32 CCATGG C^CATGG
NdeI 531 CATATG CA^TATG
NgoMIV 1000 GCCGGC G^CCGGC
PflMI 84 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1094 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 1039 GTAC GT^AC
SacI 482 GAGCTC GAGCT^C
SalI 694 GTCGAC G^TCGAC
SfcI 1122 CTACAG C^T[GA][CT]AG
SfoI 1621 GGCGCC GGC^GCC
SmaI 978 CCCGGG CCC^GGG
StuI 1397 AGGCCT AGG^CCT
TfiI 936 GATTC G^A[AT]TC
Van91I 84 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 329 CCACCCCAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 978 CCCGGG C^CCGGG
XmaI 978 CCCGGG C^CCGGG
XmnI 1227 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaI 799 CCCGAG C^[CT]CG[GA]G
978 CCCGGG
BalI 29 TGGCCA TGG^CCA
1644 TGGCCA
BbvCI 111 GCTGAGG CCTCAGC(-5/-2)
601 GCTGAGG
BpmI 610 CTGGAG CTGGAG(16/14)
1156 CTGGAG
BsoBI 799 CCCGAG C^[CT]CG[GA]G
978 CCCGGG
BspMI 917 ACCTGC ACCTGC(4/8)
1210 ACCTGC
EcoNI 90 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
609 CCTGGAGGAGG
EcoT14I 32 CCATGG C^C[AT][AT]GG
626 CCAAGG
HgaI 455 GACGC GACGC(5/10)
1245 GACGC
MaeIII 78 GTCAC ^GT[ACGT]AC
664 GTGAC
MluNI 29 TGGCCA TGG^CCA
1644 TGGCCA
MscI 29 TGGCCA TGG^CCA
1644 TGGCCA
NotI 890 GCGGCCGC GC^GGCCGC
1307 GCGGCCGC
PvuI 1100 CGATCG CGAT^CG
1365 CGATCG
SexAI 1250 ACCTGGT A^CC[AT]GGT
1502 ACCTGGT
StyI 32 CCATGG C^C[AT][AT]GG
626 CCAAGG
Tsp45I 78 GTCAC ^GT[GC]AC
664 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AluI Alw44I Aor51HI
ApaI ApaLI AspHI AvaII BanI
BanII BbvI BcnI BmyI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco47III Eco52I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI KspI MaeII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
SacII Sau3AI Sau96I ScrFI SfaNI
SgrAI TaqI TseI TspRI TthHB8I