Restriction map for
pF1KB5136
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaBI BsaHI
BsaJI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT14I EcoT22I FauI FbaI FseI
HaeII HapII Hin1I HindIII HpaII
KasI KpnI Ksp632I KspI MamI
MflI MluI MroI MslI MspA1I
MspI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AciI 335 CCGC CCGC(-3/-1)
AclI 644 AACGTT AA^CGTT
AluI 263 AGCT AG^CT
AlwNI 576 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 503 GAGCAC G[AT]GC[AT]^C
AviII 451 TGCGCA TGC^GCA
BbsI 330 GTCTTC GAAGAC(2/6)
BfaI 190 CTAG C^TAG
BpuAI 330 GTCTTC GAAGAC(2/6)
BsaAI 686 CACGTA [CT]AC^GT[GA]
BsaI 632 GAGACC GGTCTC(1/5)
BseRI 563 GAGGAG GAGGAG(10/8)
BsgI 265 CTGCAC GTGCAG(16/14)
BsiHKAI 503 GAGCAC G[AT]GC[AT]^C
BsiYI 132 CCGATCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 132 CCGATCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 179 GGGAC GGGAC(10/14)
BspMI 571 ACCTGC ACCTGC(4/8)
BstAPI 575 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 113 TTCGAA TT^CGAA
BstF5I 29 GGATG GGATG(2/0)
CfoI 452 GCGC GCG^C
DdeI 416 CTCAG C^T[ACGT]AG
DpnII 134 GATC ^GATC
DraII 179 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 179 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 79 GATATC GAT^ATC
FokI 29 GGATG GGATG(9/13)
FspI 451 TGCGCA TGC^GCA
HgaI 487 GACGC GACGC(5/10)
HhaI 452 GCGC GCG^C
HinP1I 452 GCGC G^CGC
HincII 619 GTTAAC GT[CT]^[GA]AC
HindII 619 GTTAAC GT[CT]^[GA]AC
HpaI 619 GTTAAC GTT^AAC
HphI 141 GGTGA GGTGA(8/7)
MaeI 190 CTAG C^TAG
MboI 134 GATC ^GATC
MfeI 219 CAATTG C^AATTG
MunI 219 CAATTG C^AATTG
NdeII 134 GATC ^GATC
NspV 113 TTCGAA TT^CGAA
PleI 381 GACTC GAGTC(4/5)
PpuMI 179 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 644 AACGTT AA^CGTT
PstI 573 CTGCAG CTGCA^G
Sau3AI 134 GATC ^GATC
SfcI 573 CTGCAG C^T[GA][CT]AG
SfuI 113 TTCGAA TT^CGAA
SspI 67 AATATT AAT^ATT
TfiI 116 GAATC G^A[AT]TC
Tsp45I 7 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 763 GTAC GT^AC
774 GTAC
AspI 303 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
634 GACCAAGTC
AvaII 180 GGACC G^G[AT]CC
526 GGACC
BalI 405 TGGCCA TGG^CCA
550 TGGCCA
BbvI 264 GCTGC GCAGC(8/12)
449 GCTGC
BmyI 313 GTGCCC G[AGT]GC[ACT]^C
503 GAGCAC
BsmAI 632 GAGAC GTCTC(1/5)
43 GTCTC
Bsp1286I 313 GTGCCC G[AGT]GC[ACT]^C
503 GAGCAC
EaeI 405 TGGCCA [CT]^GGCC[GA]
550 TGGCCA
Fnu4HI 264 GCTGC GC^[ACGT]GC
449 GCTGC
HinfI 116 GAATC G^A[ACGT]TC
381 GACTC
ItaI 264 GCTGC GC^[ACGT]GC
449 GCTGC
MaeII 645 ACGT A^CGT
687 ACGT
MboII 91 GAAGA GAAGA(8/7)
331 TCTTC
MluNI 405 TGGCCA TGG^CCA
550 TGGCCA
MscI 405 TGGCCA TGG^CCA
550 TGGCCA
MseI 620 TTAA T^TAA
719 TTAA
NlaIII 171 CATG CATG^
209 CATG
PflFI 303 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
634 GACCAAGTC
RsaI 763 GTAC GT^AC
774 GTAC
TaqI 83 TCGA T^CGA
114 TCGA
Tru9I 620 TTAA T^TAA
719 TTAA
TseI 264 GCTGC G^C[AT]GC
449 GCTGC
Tth111I 303 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
634 GACCAAGTC
TthHB8I 83 TCGA T^CGA
114 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
BsrI BssKI BstNI Cac8I Cfr13I
EcoRII HaeIII MaeIII MnlI MvaI
MwoI NlaIV Sau96I ScrFI Tsp509I
TspRI