Restriction map for
pF1KB4988
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BciVI BfrI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI KasI KpnI
Ksp632I KspI MamI MluI MluNI
MroI MscI MseI MvnI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspI StuI StyI
SwaI TaqI TfiI Tru9I Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 446 GAATTC [GA]^AATT[CT]
AlwI 718 GATCC GGATC(4/5)
AlwNI 667 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 446 GAATTC [GA]^AATT[CT]
AspHI 730 GAGCTC G[AT]GC[AT]^C
BbsI 100 GTCTTC GAAGAC(2/6)
BbvCI 580 GCTGAGG CCTCAGC(-5/-2)
BcgI 570 CGACCTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 167 TGATCA T^GATCA
BpuAI 100 GTCTTC GAAGAC(2/6)
BsgI 702 GTGCAG GTGCAG(16/14)
BsiHKAI 730 GAGCTC G[AT]GC[AT]^C
BsmFI 508 GTCCC GGGAC(10/14)
BsmI 353 GCATTC GAATGC(1/-1)
BspHI 623 TCATGA T^CATGA
BstAPI 74 GCATTTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 555 CCTGG CC^[AT]GG
BstYI 717 AGATCC [GA]^GATC[CT]
EcoRI 446 GAATTC G^AATTC
EcoRII 555 CCTGG ^CC[AT]GG
EcoT22I 628 ATGCAT ATGCA^T
FbaI 167 TGATCA T^GATCA
HphI 17 TCACC GGTGA(8/7)
MaeII 253 ACGT A^CGT
MaeIII 283 GTCAC ^GT[ACGT]AC
MfeI 540 CAATTG C^AATTG
MflI 717 AGATCC [GA]^GATC[CT]
MslI 436 CATTGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 540 CAATTG C^AATTG
MvaI 555 CCTGG CC^[AT]GG
NheI 400 GCTAGC G^CTAGC
NsiI 628 ATGCAT ATGCA^T
PstI 522 CTGCAG CTGCA^G
RcaI 623 TCATGA T^CATGA
SacI 730 GAGCTC GAGCT^C
SphI 677 GCATGC GCATG^C
Tsp45I 283 GTCAC ^GT[GC]AC
XcmI 26 CCAAGACCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 717 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 144 GTAC GT^AC
421 GTAC
BanII 182 GAGCCC G[GA]GC[CT]^C
730 GAGCTC
BcnI 228 CCGGG CC^[GC]GG
405 CCGGG
BfaI 401 CTAG C^TAG
598 CTAG
BglI 5 GCCAGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
643 GCCGCACTGGC
BmyI 182 GAGCCC G[AGT]GC[ACT]^C
730 GAGCTC
BseRI 21 CTCCTC GAGGAG(10/8)
733 CTCCTC
BsiYI 221 CCACAATCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
267 CCTGCTCTTGG
BslI 221 CCACAATCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
267 CCTGCTCTTGG
BsmAI 426 GAGAC GTCTC(1/5)
529 GAGAC
Bsp1286I 182 GAGCCC G[AGT]GC[ACT]^C
730 GAGCTC
Bsp1407I 143 TGTACA T^GTACA
420 TGTACA
BsrGI 143 TGTACA T^GTACA
420 TGTACA
BstF5I 153 CATCC GGATG(2/0)
264 CATCC
FokI 153 CATCC GGATG(9/13)
264 CATCC
HapII 228 CCGG C^CGG
405 CCGG
HpaII 228 CCGG C^CGG
405 CCGG
MaeI 401 CTAG C^TAG
598 CTAG
MspI 228 CCGG C^CGG
405 CCGG
NciI 228 CCGGG CC^[GC]GG
405 CCGGG
NspI 630 GCATGT [GA]CATG^[CT]
677 GCATGC
RsaI 144 GTAC GT^AC
421 GTAC
SspBI 143 TGTACA T^GTACA
420 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI BbvI BsrI BssKI
Cac8I Cfr13I DdeI DpnII Fnu4HI
HaeIII ItaI MboI MboII MnlI
MspA1I MwoI NdeII NlaIII NlaIV
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI TseI Tsp509I TspRI