Restriction map for
pF1KB4987
##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvrII BalI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaI BseAI
BsgI BsiEI BsiWI BsmBI BspDI
BspEI BspLU11I BspMI BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI FbaI
FseI HincII HindII HindIII HpaI
KpnI MaeI MfeI MluI MluNI
MroI MscI MunI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PflMI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SalI SapI ScaI
SexAI SfiI SfuI SmaI SnaBI
SpeI SspI SwaI Tth111I Van91I
XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 964 GACGTC GACGT^C
AccII 159 CGCG CG^CG
AflIII 1190 ACGTGT A^C[GA][CT]GT
AgeI 1279 ACCGGT A^CCGGT
ApaI 486 GGGCCC GGGCC^C
Asp700I 1371 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 395 TGCGCA TGC^GCA
BamHI 939 GGATCC G^GATCC
BanI 1033 GGCGCC G^G[CT][GA]CC
BbeI 1033 GGCGCC GGCGC^C
BglI 844 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 884 AGATCT A^GATCT
BsaBI 424 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1279 ACCGGT [AT]^CCGG[AT]
BsmI 651 GAATGC GAATGC(1/-1)
Bsp1407I 473 TGTACA T^GTACA
BspHI 1379 TCATGA T^CATGA
BsrDI 502 GCAATG GCAATG(2/0)
BsrGI 473 TGTACA T^GTACA
BstEII 514 GGTCACC G^GT[ACGT]ACC
BstPI 514 GGTCACC G^GT[ACGT]ACC
BstUI 159 CGCG CG^CG
DdeI 732 CTCAG C^T[ACGT]AG
DraIII 537 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 621 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 514 GGTCACC G^GT[ACGT]ACC
EcoRV 904 GATATC GAT^ATC
EcoT22I 505 ATGCAT ATGCA^T
FspI 395 TGCGCA TGC^GCA
HaeII 1033 GGCGCC [GA]GCGC^[CT]
HgaI 91 GCGTC GACGC(5/10)
KasI 1033 GGCGCC G^GCGCC
KspI 158 CCGCGG CCGC^GG
MamI 424 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 159 CGCG CG^CG
NarI 1033 GGCGCC GG^CGCC
NcoI 259 CCATGG C^CATGG
NsiI 505 ATGCAT ATGCA^T
PinAI 1279 ACCGGT A^CCGGT
PleI 1383 GACTC GAGTC(4/5)
RcaI 1379 TCATGA T^CATGA
SacII 158 CCGCGG CCGC^GG
SbfI 1026 CCTGCAGG CCTGCA^GG
SfoI 1033 GGCGCC GGC^GCC
SgrAI 1278 CACCGGTG C[GA]^CCGG[CT]G
SphI 1122 GCATGC GCATG^C
Sse8387I 1026 CCTGCAGG CCTGCA^GG
SspBI 473 TGTACA T^GTACA
StuI 629 AGGCCT AGG^CCT
XmnI 1371 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 964 GACGTC G[GA]^CG[CT]C
1033 GGCGCC
AlwNI 233 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
813 CAGCGACTG
AvaI 1219 CTCGAG C^[CT]CG[GA]G
1237 CTCGAG
BsaHI 964 GACGTC G[GA]^CG[CT]C
1033 GGCGCC
BseRI 1068 CTCCTC GAGGAG(10/8)
1153 GAGGAG
BsmAI 893 GAGAC GTCTC(1/5)
1142 GAGAC
BsmFI 962 GGGAC GGGAC(10/14)
72 GTCCC
BsoBI 1219 CTCGAG C^[CT]CG[GA]G
1237 CTCGAG
BsrFI 370 ACCGGC [GA]^CCGG[CT]
1279 ACCGGT
BsrI 209 CCAGT ACTGG(1/-1)
909 CCAGT
BstYI 884 AGATCT [GA]^GATC[CT]
939 GGATCC
Cfr10I 370 ACCGGC [GA]^CCGG[CT]
1279 ACCGGT
DsaI 158 CCGCGG C^C[GA][CT]GG
259 CCATGG
EaeI 372 CGGCCA [CT]^GGCC[GA]
1091 CGGCCA
EarI 492 CTCTTC CTCTTC(1/4)
241 GAAGAG
Hin1I 964 GACGTC G[GA]^CG[CT]C
1033 GGCGCC
Ksp632I 492 CTCTTC CTCTTC(1/4)
241 GAAGAG
MflI 884 AGATCT [GA]^GATC[CT]
939 GGATCC
MseI 136 TTAA T^TAA
560 TTAA
MslI 576 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
720 CATGGGGATG
MspA1I 158 CCGCGG C[AC]G^C[GT]G
1263 CAGCGG
PaeR7I 1219 CTCGAG C^TCGAG
1237 CTCGAG
PpuMI 70 AGGTCCC [GA]G^G[AT]CC[CT]
611 AGGACCT
Tru9I 136 TTAA T^TAA
560 TTAA
Tsp45I 515 GTCAC ^GT[GC]AC
1354 GTCAC
Tsp509I 105 AATT ^AATT
333 AATT
XhoI 1219 CTCGAG C^TCGAG
1237 CTCGAG
XhoII 884 AGATCT [GA]^GATC[CT]
939 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DpnII DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI TseI TspRI
TthHB8I