Restriction map for
pF1KB4894
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AlwNI
Aor51HI ApoI AscI AseI AsnI
Asp718I AspEI AviII AvrII BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
MamI MfeI MroI MseI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI SapI SbfI SexAI
SfaNI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
XbaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Alw44I 169 GTGCAC G^TGCAC
ApaI 744 GGGCCC GGGCC^C
ApaLI 169 GTGCAC G^TGCAC
AspI 177 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 719 TGGCCA TGG^CCA
BfaI 547 CTAG C^TAG
BsaI 283 GAGACC GGTCTC(1/5)
BsrBI 1000 GAGCGG CCGCTC(-3/-3)
BstAPI 1211 GCATGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 257 CCACGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoRV 859 GATATC GAT^ATC
EcoT14I 1058 CCTTGG C^C[AT][AT]GG
HinfI 181 GAGTC G^A[ACGT]TC
KspI 50 CCGCGG CCGC^GG
MaeI 547 CTAG C^TAG
MboII 1118 TCTTC GAAGA(8/7)
MluI 231 ACGCGT A^CGCGT
MluNI 719 TGGCCA TGG^CCA
MscI 719 TGGCCA TGG^CCA
MslI 370 CACGGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 198 CTCGAG C^TCGAG
PflFI 177 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 81 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 181 GAGTC GAGTC(4/5)
PvuII 331 CAGCTG CAG^CTG
SacII 50 CCGCGG CCGC^GG
ScaI 1022 AGTACT AGT^ACT
SfcI 222 CTACAG C^T[GA][CT]AG
SmaI 748 CCCGGG CCC^GGG
StyI 1058 CCTTGG C^C[AT][AT]GG
Tsp45I 349 GTGAC ^GT[GC]AC
Tth111I 177 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 81 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 198 CTCGAG C^TCGAG
XmaCI 748 CCCGGG C^CCGGG
XmaI 748 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AocI 138 CCTGAGG CC^T[ACGT]AGG
1006 CCTGAGG
Asp700I 18 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
438 GAAGGTGTTC
AspHI 169 GTGCAC G[AT]GC[AT]^C
201 GAGCAC
BamHI 785 GGATCC G^GATCC
1173 GGATCC
BbrPI 166 CACGTG CAC^GTG
829 CACGTG
BsaAI 166 CACGTG [CT]AC^GT[GA]
829 CACGTG
BsiEI 47 CGACCG CG[GA][CT]^CG
570 CGACCG
BsiHKAI 169 GTGCAC G[AT]GC[AT]^C
201 GAGCAC
BsmAI 283 GAGAC GTCTC(1/5)
550 GTCTC
BsmFI 1193 GGGAC GGGAC(10/14)
418 GTCCC
BsrFI 407 ACCGGC [GA]^CCGG[CT]
738 GCCGGT
BssHII 147 GCGCGC G^CGCGC
149 GCGCGC
BstYI 785 GGATCC [GA]^GATC[CT]
1173 GGATCC
Bsu36I 138 CCTGAGG CC^T[ACGT]AGG
1006 CCTGAGG
Cfr10I 407 ACCGGC [GA]^CCGG[CT]
738 GCCGGT
Eco81I 138 CCTGAGG CC^T[ACGT]AGG
1006 CCTGAGG
HgaI 291 GACGC GACGC(5/10)
56 GCGTC
MaeIII 349 GTGAC ^GT[ACGT]AC
536 GTAAC
MflI 785 GGATCC [GA]^GATC[CT]
1173 GGATCC
PmaCI 166 CACGTG CAC^GTG
829 CACGTG
PmlI 166 CACGTG CAC^GTG
829 CACGTG
XcmI 875 CCAAGCTGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
962 CCAGCTTCCTGCTGG
XhoII 785 GGATCC [GA]^GATC[CT]
1173 GGATCC
XmnI 18 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
438 GAAGGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AvaI AvaII BanI
BanII BbeI BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiYI BslI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HpaII
HphI ItaI KasI MaeII MboI
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PpuMI RsaI Sau3AI Sau96I
ScrFI SfoI SmlI TaqI TseI
TspRI TthHB8I