Restriction map for
pF1KB4374
##################################
# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BciVI
BclI BfrI BglI BglII BlnI
BpmI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstZ17I CfoI Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MaeIII MamI MfeI MluI
MroI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NsiI NspI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tsp45I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 780 GTCTAC GT^[AC][GT]AC
AccII 1147 CGCG CG^CG
AocI 558 CCTCAGG CC^T[ACGT]AGG
BalI 477 TGGCCA TGG^CCA
BbsI 919 GAAGAC GAAGAC(2/6)
BcgI 879 GCATGAAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1060 CTAG C^TAG
BlpI 874 GCTGAGC GC^T[ACGT]AGC
BmyI 259 GGGCAC G[AGT]GC[ACT]^C
Bpu1102I 874 GCTGAGC GC^T[ACGT]AGC
BpuAI 919 GAAGAC GAAGAC(2/6)
BsaI 306 GAGACC GGTCTC(1/5)
Bsp1286I 259 GGGCAC G[AGT]GC[ACT]^C
BstBI 1042 TTCGAA TT^CGAA
BstUI 1147 CGCG CG^CG
BstXI 362 CCATTGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1170 GGATCT [GA]^GATC[CT]
Bsu36I 558 CCTCAGG CC^T[ACGT]AGG
CelII 874 GCTGAGC GC^T[ACGT]AGC
DraI 459 TTTAAA TTT^AAA
EaeI 477 TGGCCA [CT]^GGCC[GA]
EarI 80 CTCTTC CTCTTC(1/4)
Eco81I 558 CCTCAGG CC^T[ACGT]AGG
EcoRI 272 GAATTC G^AATTC
HaeIII 478 GGCC GG^CC
Ksp632I 80 CTCTTC CTCTTC(1/4)
MaeI 1060 CTAG C^TAG
MaeII 489 ACGT A^CGT
MflI 1170 GGATCT [GA]^GATC[CT]
MluNI 477 TGGCCA TGG^CCA
MscI 477 TGGCCA TGG^CCA
MslI 408 CACTTCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1147 CGCG CG^CG
MwoI 1063 GCACTACAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI 1059 GCTAGC G^CTAGC
NspV 1042 TTCGAA TT^CGAA
PleI 836 GAGTC GAGTC(4/5)
ScaI 635 AGTACT AGT^ACT
SfuI 1042 TTCGAA TT^CGAA
XhoII 1170 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 526 GATCC GGATC(4/5)
1170 GGATC
AseI 397 ATTAAT AT^TAAT
451 ATTAAT
AsnI 397 ATTAAT AT^TAAT
451 ATTAAT
BcnI 627 CCCGG CC^[GC]GG
1193 CCGGG
BseRI 1089 CTCCTC GAGGAG(10/8)
1222 GAGGAG
BsgI 517 CTGCAC GTGCAG(16/14)
193 GTGCAG
BsmI 1039 GCATTC GAATGC(1/-1)
1154 GCATTC
BsrDI 9 GCAATG GCAATG(2/0)
209 GCAATG
DsaI 47 CCGTGG C^C[GA][CT]GG
1166 CCGTGG
EcoNI 88 CCTCTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1029 CCTGGGACAGG
FauI 1145 CCCGC CCCGC(4/6)
762 GCGGG
HapII 628 CCGG C^CGG
1193 CCGG
HpaII 628 CCGG C^CGG
1193 CCGG
MspI 628 CCGG C^CGG
1193 CCGG
NciI 627 CCCGG CC^[GC]GG
1193 CCGGG
PflMI 114 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
165 CCACCTGATGG
PshBI 397 ATTAAT AT^TAAT
451 ATTAAT
TaqI 412 TCGA T^CGA
1043 TCGA
TspRI 119 CAGTG CA[GC]TG[ACGT][ACGT]^
1218 CACTG
TthHB8I 412 TCGA T^CGA
1043 TCGA
Van91I 114 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
165 CCACCTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BsaJI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HindIII HinfI HphI ItaI MboI
MboII MnlI MseI MvaI NdeII
NlaIII NlaIV PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp509I