Restriction map for
pF1KB4334
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BalI BbeI BbrPI
BbvCI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
FbaI FseI FspI HaeII HgaI
Hin1I KasI KpnI KspI MaeII
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 1167 GAATTC [GA]^AATT[CT]
AocI 492 CCTCAGG CC^T[ACGT]AGG
ApoI 1167 GAATTC [GA]^AATT[CT]
BamHI 302 GGATCC G^GATCC
BcgI 1329 CGATGTGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 730 GGATAC GTATCC(6/5)
BfaI 821 CTAG C^TAG
BpmI 964 CTGGAG CTGGAG(16/14)
BsaBI 192 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 601 ACCGGA [AT]^CCGG[AT]
BseRI 1287 GAGGAG GAGGAG(10/8)
BsgI 522 CTGCAC GTGCAG(16/14)
BsmBI 115 GAGACG CGTCTC(1/5)
BsmI 1335 GAATGC GAATGC(1/-1)
BstAPI 811 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 302 GGATCC [GA]^GATC[CT]
Bsu36I 492 CCTCAGG CC^T[ACGT]AGG
CfoI 71 GCGC GCG^C
DsaI 554 CCACGG C^C[GA][CT]GG
Eco81I 492 CCTCAGG CC^T[ACGT]AGG
EcoNI 1222 CCTCGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1167 GAATTC G^AATTC
EcoT14I 1040 CCAAGG C^C[AT][AT]GG
HhaI 71 GCGC GCG^C
HinP1I 71 GCGC G^CGC
HindIII 1021 AAGCTT A^AGCTT
HpaI 1414 GTTAAC GTT^AAC
MaeI 821 CTAG C^TAG
MamI 192 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 302 GGATCC [GA]^GATC[CT]
PpuMI 171 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 619 GAGCTC GAGCT^C
SmaI 91 CCCGGG CCC^GGG
StyI 1040 CCAAGG C^C[AT][AT]GG
XcmI 209 CCAGCATCGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 302 GGATCC [GA]^GATC[CT]
XmaCI 91 CCCGGG C^CCGGG
XmaI 91 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 98 ACATGT A^C[GA][CT]GT
920 ACATGT
AhdI 376 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1345 GACAGATGGTC
AspEI 376 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1345 GACAGATGGTC
AspHI 619 GAGCTC G[AT]GC[AT]^C
643 GAGCAC
BanI 351 GGTGCC G^G[CT][GA]CC
682 GGTGCC
BanII 454 GGGCTC G[GA]GC[CT]^C
619 GAGCTC
BsiHKAI 619 GAGCTC G[AT]GC[AT]^C
643 GAGCAC
BspLU11I 98 ACATGT A^CATGT
920 ACATGT
Eam1105I 376 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1345 GACAGATGGTC
EarI 717 GAAGAG CTCTTC(1/4)
998 GAAGAG
EcoT22I 480 ATGCAT ATGCA^T
809 ATGCAT
HphI 429 TCACC GGTGA(8/7)
464 TCACC
Ksp632I 717 GAAGAG CTCTTC(1/4)
998 GAAGAG
NsiI 480 ATGCAT ATGCA^T
809 ATGCAT
PaeR7I 657 CTCGAG C^TCGAG
1223 CTCGAG
SmlI 657 CTCGAG C^T[CT][GA]AG
1223 CTCGAG
SphI 478 GCATGC GCATG^C
1217 GCATGC
SspI 269 AATATT AAT^ATT
1096 AATATT
Tsp45I 400 GTGAC ^GT[GC]AC
699 GTCAC
XhoI 657 CTCGAG C^TCGAG
1223 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BbsI BbvI BcnI BmyI
BpuAI BsaI BsaJI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII ItaI
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I