Restriction map for
pF1KB4295
##################################
# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
AvrII BalI BbeI BbrPI BbsI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HphI
KasI Ksp632I KspI MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 619 GGTACC G^GTACC
Asp718I 619 GGTACC G^GTACC
AspHI 256 GTGCTC G[AT]GC[AT]^C
AvaII 668 GGACC G^G[AT]CC
BamHI 537 GGATCC G^GATCC
BanI 619 GGTACC G^G[CT][GA]CC
BanII 242 GAGCCC G[GA]GC[CT]^C
BbvI 695 GCAGC GCAGC(8/12)
BglII 462 AGATCT A^GATCT
BsaJI 245 CCCAGG C^C[ACGT][ACGT]GG
BsaWI 604 TCCGGT [AT]^CCGG[AT]
BseRI 239 GAGGAG GAGGAG(10/8)
BsiHKAI 256 GTGCTC G[AT]GC[AT]^C
BsiYI 272 CCACAGATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 272 CCACAGATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 188 GGGAC GGGAC(10/14)
BsmI 552 GCATTC GAATGC(1/-1)
BspMI 196 GCAGGT ACCTGC(4/8)
BssKI 246 CCAGG ^CC[ACGT]GG
BssSI 259 CTCGTG CACGAG(-5/-1)
BstF5I 378 GGATG GGATG(2/0)
BstNI 246 CCAGG CC^[AT]GG
BstXI 26 CCATAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 668 GGACC G^G[ACGT]CC
EcoRI 612 GAATTC G^AATTC
EcoRII 246 CCAGG ^CC[AT]GG
Fnu4HI 695 GCAGC GC^[ACGT]GC
FokI 378 GGATG GGATG(9/13)
HaeIII 228 GGCC GG^CC
HapII 605 CCGG C^CGG
HpaII 605 CCGG C^CGG
ItaI 695 GCAGC GC^[ACGT]GC
KpnI 619 GGTACC GGTAC^C
MslI 27 CATAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 696 CAGCTG C[AC]G^C[GT]G
MspI 605 CCGG C^CGG
MvaI 246 CCAGG CC^[AT]GG
MwoI 695 GCAGCTGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PstI 194 CTGCAG CTGCA^G
PvuII 696 CAGCTG CAG^CTG
Sau96I 668 GGACC G^G[ACGT]CC
ScrFI 246 CCAGG CC^[ACGT]GG
SfaNI 214 GATGC GCATC(5/9)
StuI 227 AGGCCT AGG^CCT
TaqI 237 TCGA T^CGA
TseI 695 GCAGC G^C[AT]GC
Tsp45I 599 GTCAC ^GT[GC]AC
TthHB8I 237 TCGA T^CGA
XcmI 26 CCATAAAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AfaI 349 GTAC GT^AC
620 GTAC
AlwI 538 GATCC GGATC(4/5)
537 GGATC
BbvCI 406 GCTGAGG CCTCAGC(-5/-2)
532 GCTGAGG
BmyI 242 GAGCCC G[AGT]GC[ACT]^C
256 GTGCTC
Bsp1286I 242 GAGCCC G[AGT]GC[ACT]^C
256 GTGCTC
BsrI 414 ACTGG ACTGG(1/-1)
302 CCAGT
BstYI 462 AGATCT [GA]^GATC[CT]
537 GGATCC
MaeIII 563 GTAAC ^GT[ACGT]AC
599 GTCAC
MflI 462 AGATCT [GA]^GATC[CT]
537 GGATCC
RsaI 349 GTAC GT^AC
620 GTAC
XhoII 462 AGATCT [GA]^GATC[CT]
537 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AcsI AluI ApoI DdeI DpnII
HinfI MboI MboII MnlI MseI
NdeII NlaIV Sau3AI SfcI TfiI
Tru9I Tsp509I TspRI