Restriction map for
pF1KB3985
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BbeI
BbrPI BbvCI BcgI BclI BfrI
BglII BlnI BsaAI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BspDI BspEI BspLU11I BsrDI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I EcoRI EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI MluI
MluNI MroI MscI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 553 GTCTAC GT^[AC][GT]AC
Alw44I 54 GTGCAC G^TGCAC
AocI 420 CCTGAGG CC^T[ACGT]AGG
Aor51HI 39 AGCGCT AGC^GCT
ApaI 513 GGGCCC GGGCC^C
ApaLI 54 GTGCAC G^TGCAC
Asp700I 597 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 202 CCCGGG C^[CT]CG[GA]G
BbsI 3 GAAGAC GAAGAC(2/6)
BciVI 235 GGATAC GTATCC(6/5)
BfaI 70 CTAG C^TAG
BglI 83 GCCTCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 690 GCTCAGC GC^T[ACGT]AGC
BpmI 724 CTGGAG CTGGAG(16/14)
Bpu1102I 690 GCTCAGC GC^T[ACGT]AGC
BpuAI 3 GAAGAC GAAGAC(2/6)
BsaBI 781 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 316 CTGCAC GTGCAG(16/14)
BsmAI 655 GAGAC GTCTC(1/5)
BsmI 435 GCATTC GAATGC(1/-1)
BsoBI 202 CCCGGG C^[CT]CG[GA]G
BspHI 605 TCATGA T^CATGA
BsrBI 100 GAGCGG CCGCTC(-3/-3)
BstAPI 309 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 294 GGTGACC G^GT[ACGT]ACC
BstPI 294 GGTGACC G^GT[ACGT]ACC
Bsu36I 420 CCTGAGG CC^T[ACGT]AGG
CelII 690 GCTCAGC GC^T[ACGT]AGC
DraIII 48 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 643 CTCTTC CTCTTC(1/4)
Eco47III 39 AGCGCT AGC^GCT
Eco81I 420 CCTGAGG CC^T[ACGT]AGG
EcoNI 573 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 294 GGTGACC G^GT[ACGT]ACC
EcoRV 675 GATATC GAT^ATC
EcoT14I 18 CCATGG C^C[AT][AT]GG
Ksp632I 643 CTCTTC CTCTTC(1/4)
MaeI 70 CTAG C^TAG
MamI 781 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 716 CAATTG C^AATTG
MseI 911 TTAA T^TAA
MunI 716 CAATTG C^AATTG
NaeI 171 GCCGGC GCC^GGC
NcoI 18 CCATGG C^CATGG
NgoMIV 171 GCCGGC G^CCGGC
NspI 527 ACATGC [GA]CATG^[CT]
PleI 905 GAGTC GAGTC(4/5)
PpuMI 206 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 605 TCATGA T^CATGA
SapI 642 GCTCTTC GCTCTTC(1/4)
SmaI 202 CCCGGG CCC^GGG
SspI 934 AATATT AAT^ATT
StuI 599 AGGCCT AGG^CCT
StyI 18 CCATGG C^C[AT][AT]GG
TfiI 967 GAATC G^A[AT]TC
Tru9I 911 TTAA T^TAA
Tsp509I 717 AATT ^AATT
XmaCI 202 CCCGGG C^CCGGG
XmaI 202 CCCGGG C^CCGGG
XmnI 597 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 147 GGCGTC G[GA]^CG[CT]C
373 GACGCC
AlwNI 279 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
580 CAGGTGCTG
BamHI 452 GGATCC G^GATCC
780 GGATCC
BanI 493 GGCACC G^G[CT][GA]CC
759 GGTGCC
BsaHI 147 GGCGTC G[GA]^CG[CT]C
373 GACGCC
Bsp1407I 74 TGTACA T^GTACA
838 TGTACA
BspMI 473 ACCTGC ACCTGC(4/8)
563 ACCTGC
BsrFI 157 ACCGGC [GA]^CCGG[CT]
171 GCCGGC
BsrGI 74 TGTACA T^GTACA
838 TGTACA
BstYI 452 GGATCC [GA]^GATC[CT]
780 GGATCC
Cfr10I 157 ACCGGC [GA]^CCGG[CT]
171 GCCGGC
HaeII 39 AGCGCT [GA]GCGC^[CT]
197 AGCGCC
Hin1I 147 GGCGTC G[GA]^CG[CT]C
373 GACGCC
HinfI 905 GAGTC G^A[ACGT]TC
967 GAATC
KspI 212 CCGCGG CCGC^GG
220 CCGCGG
MaeII 401 ACGT A^CGT
482 ACGT
MflI 452 GGATCC [GA]^GATC[CT]
780 GGATCC
MspA1I 212 CCGCGG C[AC]G^C[GT]G
220 CCGCGG
PflMI 18 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
680 CCAACACTTGG
SacI 640 GAGCTC GAGCT^C
727 GAGCTC
SacII 212 CCGCGG CCGC^GG
220 CCGCGG
SspBI 74 TGTACA T^GTACA
838 TGTACA
Van91I 18 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
680 CCAACACTTGG
XhoII 452 GGATCC [GA]^GATC[CT]
780 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BanII BbvI BcnI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MaeIII MboI
MboII MnlI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TseI Tsp45I
TspRI