##################################
# NO CUT ENZYMES (  204 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BssHII      
BssSI       BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HincII      HindII      HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MflI        MluI        MluNI       
MroI        MscI        MseI        MspA1I      MspI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        StyI        SwaI        
Tru9I       Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AfaI          255  GTAC                 GT^AC
Asp700I       274  GAATCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BsaBI          32  GATTGTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         152  GAGGAG               GAGGAG(10/8)
BsiYI         532  CCTTTGCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          532  CCTTTGCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         381  GAGAC                GTCTC(1/5)
Bsp1407I      254  TGTACA               T^GTACA
BspDI         310  ATCGAT               AT^CGAT
BsrGI         254  TGTACA               T^GTACA
BsrI          494  CCAGT                ACTGG(1/-1)
BssKI         538  CCTGG                ^CC[ACGT]GG
Bst1107I      124  GTATAC               GTA^TAC
BstNI         538  CCTGG                CC^[AT]GG
BstZ17I       124  GTATAC               GTA^TAC
ClaI          310  ATCGAT               AT^CGAT
DpnII         457  GATC                 ^GATC
EcoRII        538  CCTGG                ^CC[AT]GG
HgaI          391  GACGC                GACGC(5/10)
HindIII       525  AAGCTT               A^AGCTT
HinfI         274  GAATC                G^A[ACGT]TC
HphI          444  GGTGA                GGTGA(8/7)
MamI           32  GATTGTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          457  GATC                 ^GATC
MfeI          341  CAATTG               C^AATTG
MunI          341  CAATTG               C^AATTG
MvaI          538  CCTGG                CC^[AT]GG
NdeII         457  GATC                 ^GATC
NlaIII        129  CATG                 CATG^
RsaI          255  GTAC                 GT^AC
Sau3AI        457  GATC                 ^GATC
ScrFI         538  CCTGG                CC^[ACGT]GG
SfcI          193  CTACAG               C^T[GA][CT]AG
SspBI         254  TGTACA               T^GTACA
TfiI          274  GAATC                G^A[AT]TC
Tsp45I        445  GTGAC                ^GT[GC]AC
XmnI          274  GAATCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AccI          124  GTATAC               GT^[AC][GT]AC
              191  GTCTAC              
BsaJI         150  CCGAGG               C^C[ACGT][ACGT]GG
              538  CCTGGG              
DdeI          374  CTGAG                C^T[ACGT]AG
              569  CTGAG               
MslI          172  CATCTTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              404  CACAGCAGTG          
TaqI          311  TCGA                 T^CGA
              365  TCGA                
TspRI         258  CAGTG                CA[GC]TG[ACGT][ACGT]^
              409  CAGTG               
TthHB8I       311  TCGA                 T^CGA
              365  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

AluI        BbvI        Fnu4HI      ItaI        MaeIII      
MboII       MnlI        MwoI        TseI        Tsp509I