Restriction map for
pF1KB3968
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AcyI AflII Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BpmI BsaAI BsaHI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspMI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI Bsu36I ClaI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MfeI
MflI MluI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SacI
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tsp509I Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 989 TCCGGA T^CCGGA
AflIII 584 ACATGT A^C[GA][CT]GT
AgeI 497 ACCGGT A^CCGGT
AhdI 52 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 170 GGGCCC GGGCC^C
AspEI 52 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 46 CCCGAG C^[CT]CG[GA]G
BbvCI 9 GCTGAGG CCTCAGC(-5/-2)
BglI 1124 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 381 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 381 GCTCAGC GC^T[ACGT]AGC
BsaBI 282 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1286 GGTCTC GGTCTC(1/5)
BseAI 989 TCCGGA T^CCGGA
BsoBI 46 CCCGAG C^[CT]CG[GA]G
BspEI 989 TCCGGA T^CCGGA
BspLU11I 584 ACATGT A^CATGT
BsrDI 608 CATTGC GCAATG(2/0)
Bst1107I 1183 GTATAC GTA^TAC
BstZ17I 1183 GTATAC GTA^TAC
Cac8I 932 GCCTGC GC[ACGT]^[ACGT]GC
CelII 381 GCTCAGC GC^T[ACGT]AGC
CfoI 40 GCGC GCG^C
CpoI 986 CGGTCCG CG^G[AT]CCG
DraI 1090 TTTAAA TTT^AAA
DsaI 1125 CCATGG C^C[GA][CT]GG
Eam1105I 52 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 466 GAAGAG CTCTTC(1/4)
EcoNI 368 CCTGGTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1125 CCATGG C^C[AT][AT]GG
HgaI 378 GACGC GACGC(5/10)
HhaI 40 GCGC GCG^C
HinP1I 40 GCGC G^CGC
Ksp632I 466 GAAGAG CTCTTC(1/4)
MaeII 344 ACGT A^CGT
MamI 282 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 989 TCCGGA T^CCGGA
MslI 352 CACTCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 384 CAGCTG C[AC]G^C[GT]G
NcoI 1125 CCATGG C^CATGG
NdeI 356 CATATG CA^TATG
PinAI 497 ACCGGT A^CCGGT
PpuMI 158 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 384 CAGCTG CAG^CTG
RsrII 986 CGGTCCG CG^G[AT]CCG
SapI 466 GAAGAGC GCTCTTC(1/4)
SbfI 933 CCTGCAGG CCTGCA^GG
Sse8387I 933 CCTGCAGG CCTGCA^GG
StyI 1125 CCATGG C^C[AT][AT]GG
XcmI 963 CCAGTCAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 346 GTCTAC GT^[AC][GT]AC
1183 GTATAC
AlwI 960 GATCC GGATC(4/5)
1140 GGATC
BanII 170 GGGCCC G[GA]GC[CT]^C
913 GAGCCC
BbsI 771 GAAGAC GAAGAC(2/6)
186 GTCTTC
BbvI 1260 GCAGC GCAGC(8/12)
1281 GCTGC
BmyI 170 GGGCCC G[AGT]GC[ACT]^C
913 GAGCCC
BpuAI 771 GAAGAC GAAGAC(2/6)
186 GTCTTC
BsaWI 497 ACCGGT [AT]^CCGG[AT]
989 TCCGGA
Bsp1286I 170 GGGCCC G[AGT]GC[ACT]^C
913 GAGCCC
BsrFI 33 GCCGGT [GA]^CCGG[CT]
497 ACCGGT
Cfr10I 33 GCCGGT [GA]^CCGG[CT]
497 ACCGGT
Fnu4HI 1260 GCAGC GC^[ACGT]GC
1281 GCTGC
ItaI 1260 GCAGC GC^[ACGT]GC
1281 GCTGC
MseI 308 TTAA T^TAA
1091 TTAA
NspI 536 GCATGT [GA]CATG^[CT]
584 ACATGT
TaqI 570 TCGA T^CGA
1298 TCGA
Tru9I 308 TTAA T^TAA
1091 TTAA
TseI 1260 GCAGC G^C[AT]GC
1281 GCTGC
TthHB8I 570 TCGA T^CGA
1298 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BalI BcnI BsaJI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FokI HaeIII
HapII HinfI HpaII HphI MaeIII
MboI MboII MluNI MnlI MscI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI Tsp45I TspRI