##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AgeI        
AhdI        AluI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmAI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BsrDI       BsrFI       BsrGI       BsrI        
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DdeI        DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        Hin1I       HincII      
HindII      HindIII     HinfI       HpaI        HphI        
KasI        KpnI        KspI        MaeI        MaeII       
MaeIII      MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MslI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TfiI        Tru9I       Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AflIII        389  ACATGT               A^C[GA][CT]GT
AlwI          195  GATCC                GGATC(4/5)
AspHI         358  GTGCTC               G[AT]GC[AT]^C
AspI            4  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          373  CCCGGG               C^[CT]CG[GA]G
BmyI          358  GTGCTC               G[AGT]GC[ACT]^C
BpmI          256  CTGGAG               CTGGAG(16/14)
BsiHKAI       358  GTGCTC               G[AT]GC[AT]^C
BsmFI         317  GGGAC                GGGAC(10/14)
BsoBI         373  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      358  GTGCTC               G[AGT]GC[ACT]^C
BspHI          11  TCATGA               T^CATGA
BspLU11I      389  ACATGT               A^CATGT
BspMI         368  ACCTGC               ACCTGC(4/8)
BssHII        157  GCGCGC               G^CGCGC
BstYI         194  AGATCC               [GA]^GATC[CT]
DpnII         195  GATC                 ^GATC
EarI          334  GAAGAG               CTCTTC(1/4)
EcoT14I        24  CCTTGG               C^C[AT][AT]GG
HaeII         117  GGCGCT               [GA]GCGC^[CT]
HgaI           85  GACGC                GACGC(5/10)
Ksp632I       334  GAAGAG               CTCTTC(1/4)
MboI          195  GATC                 ^GATC
MflI          194  AGATCC               [GA]^GATC[CT]
MspA1I        379  CCGCTG               C[AC]G^C[GT]G
NdeII         195  GATC                 ^GATC
NspI          389  ACATGT               [GA]CATG^[CT]
PflFI           4  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI          77  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          382  CTGCAG               CTGCA^G
RcaI           11  TCATGA               T^CATGA
Sau3AI        195  GATC                 ^GATC
SfcI          382  CTGCAG               C^T[GA][CT]AG
SmaI          373  CCCGGG               CCC^GGG
StyI           24  CCTTGG               C^C[AT][AT]GG
Tth111I         4  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI           35  CCACAAAGGACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         194  AGATCC               [GA]^GATC[CT]
XmaCI         373  CCCGGG               C^CCGGG
XmaI          373  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AvaII          78  GGACC                G^G[AT]CC
              183  GGTCC               
BsrBI         106  GAGCGG               CCGCTC(-3/-3)
              172  GAGCGG              
BstF5I        300  CATCC                GGATG(2/0)
              345  CATCC               
BstNI         255  CCTGG                CC^[AT]GG
              348  CCTGG               
DraII          20  AGGCCCT              [GA]G^G[ACGT]CC[CT]
               77  AGGACCC             
EcoO109I       20  AGGCCCT              [GA]G^G[ACGT]CC[CT]
               77  AGGACCC             
EcoRII        255  CCTGG                ^CC[AT]GG
              348  CCTGG               
FokI          300  CATCC                GGATG(9/13)
              345  CATCC               
MvaI          255  CCTGG                CC^[AT]GG
              348  CCTGG               
SfaNI          54  GATGC                GCATC(5/9)
              344  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AccII       AciI        BbvI        BcnI        BsaJI       
BseRI       BsiYI       BslI        BssKI       BstUI       
Cac8I       CfoI        Cfr13I      FauI        Fnu4HI      
HaeIII      HapII       HhaI        HinP1I      HpaII       
ItaI        MboII       MnlI        MspI        MvnI        
MwoI        NciI        NlaIII      NlaIV       Sau96I      
ScrFI       TseI