Restriction map for
pF1KB3821
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BanII
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrDI BsrFI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HapII HindIII
HpaI HpaII KasI KpnI MamI
MluI MluNI MroI MscI MspI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcyI 46 GGCGTC G[GA]^CG[CT]C
AocI 829 CCTCAGG CC^T[ACGT]AGG
AseI 213 ATTAAT AT^TAAT
AsnI 213 ATTAAT AT^TAAT
AspHI 663 GAGCAC G[AT]GC[AT]^C
BamHI 385 GGATCC G^GATCC
BanI 321 GGCACC G^G[CT][GA]CC
BciVI 570 GTATCC GTATCC(6/5)
BfaI 544 CTAG C^TAG
BmyI 663 GAGCAC G[AGT]GC[ACT]^C
BsaHI 46 GGCGTC G[GA]^CG[CT]C
BsaI 287 GAGACC GGTCTC(1/5)
BseRI 694 CTCCTC GAGGAG(10/8)
BsgI 116 GTGCAG GTGCAG(16/14)
BsiEI 128 CGGCCG CG[GA][CT]^CG
BsiHKAI 663 GAGCAC G[AT]GC[AT]^C
BsmI 1155 GCATTC GAATGC(1/-1)
Bsp1286I 663 GAGCAC G[AGT]GC[ACT]^C
BspHI 293 TCATGA T^CATGA
BsrBI 1210 CCGCTC CCGCTC(-3/-3)
BssHII 36 GCGCGC G^CGCGC
BstXI 97 CCATGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 385 GGATCC [GA]^GATC[CT]
Bsu36I 829 CCTCAGG CC^T[ACGT]AGG
EagI 128 CGGCCG C^GGCCG
EclXI 128 CGGCCG C^GGCCG
Eco52I 128 CGGCCG C^GGCCG
Eco81I 829 CCTCAGG CC^T[ACGT]AGG
FauI 43 GCGGG CCCGC(4/6)
HaeII 841 AGCGCC [GA]GCGC^[CT]
HgaI 47 GCGTC GACGC(5/10)
Hin1I 46 GGCGTC G[GA]^CG[CT]C
HincII 808 GTCAAC GT[CT]^[GA]AC
HindII 808 GTCAAC GT[CT]^[GA]AC
KspI 41 CCGCGG CCGC^GG
MaeI 544 CTAG C^TAG
MaeII 494 ACGT A^CGT
MfeI 518 CAATTG C^AATTG
MflI 385 GGATCC [GA]^GATC[CT]
MslI 98 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 518 CAATTG C^AATTG
NcoI 97 CCATGG C^CATGG
NspI 153 GCATGC [GA]CATG^[CT]
PflMI 74 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 933 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 213 ATTAAT AT^TAAT
RcaI 293 TCATGA T^CATGA
SacII 41 CCGCGG CCGC^GG
SapI 1097 GAAGAGC GCTCTTC(1/4)
SphI 153 GCATGC GCATG^C
SspI 210 AATATT AAT^ATT
TaqI 1049 TCGA T^CGA
TthHB8I 1049 TCGA T^CGA
Van91I 74 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 385 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AlwNI 75 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1261 CAGCAGCTG
BpmI 81 CTGGAG CTGGAG(16/14)
871 CTGGAG
BsmFI 639 GGGAC GGGAC(10/14)
450 GTCCC
DsaI 41 CCGCGG C^C[GA][CT]GG
97 CCATGG
EaeI 128 CGGCCG [CT]^GGCC[GA]
1142 TGGCCG
MaeIII 133 GTCAC ^GT[ACGT]AC
1016 GTGAC
PleI 272 GACTC GAGTC(4/5)
1105 GAGTC
PstI 122 CTGCAG CTGCA^G
1230 CTGCAG
ScaI 875 AGTACT AGT^ACT
1052 AGTACT
SfaNI 921 GATGC GCATC(5/9)
70 GCATC
SfcI 122 CTGCAG C^T[GA][CT]AG
1230 CTGCAG
TfiI 354 GATTC G^A[AT]TC
949 GAATC
Tsp45I 133 GTCAC ^GT[GC]AC
1016 GTGAC
Tsp509I 446 AATT ^AATT
519 AATT
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BbvI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MboI MboII MnlI MseI MspA1I
MvaI MvnI MwoI NdeII NlaIII
NlaIV PvuII RsaI Sau3AI Sau96I
ScrFI SmlI StyI Tru9I TseI
TspRI