Restriction map for
pF1KB3815
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII BalI BbrPI
BciVI BclI BfrI BsaAI BseAI
BsgI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MaeII MfeI
MluI MluNI MroI MscI MseI
MslI MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tru9I Tth111I TthHB8I Van91I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 1195 AAATTC [GA]^AATT[CT]
AlwNI 206 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1195 AAATTC [GA]^AATT[CT]
AspHI 37 GAGCTC G[AT]GC[AT]^C
AvrII 561 CCTAGG C^CTAGG
BamHI 705 GGATCC G^GATCC
BbeI 773 GGCGCC GGCGC^C
BbsI 214 GTCTTC GAAGAC(2/6)
BbvCI 631 GCTGAGG CCTCAGC(-5/-2)
BcgI 89 GCAGCATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 494 AGATCT A^GATCT
BlnI 561 CCTAGG C^CTAGG
BpuAI 214 GTCTTC GAAGAC(2/6)
BsaBI 150 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 885 GGTCTC GGTCTC(1/5)
BsaWI 882 TCCGGT [AT]^CCGG[AT]
BsiHKAI 37 GAGCTC G[AT]GC[AT]^C
BsmBI 928 GAGACG CGTCTC(1/5)
BstEII 636 GGTCACC G^GT[ACGT]ACC
BstPI 636 GGTCACC G^GT[ACGT]ACC
BstXI 890 CCATCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 930 GACGGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 932 CGGCCA [CT]^GGCC[GA]
EcoO65I 636 GGTCACC G^GT[ACGT]ACC
EcoT14I 561 CCTAGG C^C[AT][AT]GG
FauI 970 CCCGC CCCGC(4/6)
HgaI 954 GACGC GACGC(5/10)
KasI 773 GGCGCC G^GCGCC
MamI 150 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 773 GGCGCC GG^CGCC
NspI 861 GCATGT [GA]CATG^[CT]
PleI 514 GAGTC GAGTC(4/5)
SacI 37 GAGCTC GAGCT^C
SfcI 203 CTACAG C^T[GA][CT]AG
SfoI 773 GGCGCC GGC^GCC
StuI 295 AGGCCT AGG^CCT
StyI 561 CCTAGG C^C[AT][AT]GG
TfiI 125 GAATC G^A[AT]TC
Tsp509I 1196 AATT ^AATT
XbaI 924 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 201 GTCTAC GT^[AC][GT]AC
939 GTCTAC
AcyI 773 GGCGCC G[GA]^CG[CT]C
954 GACGCC
BanII 37 GAGCTC G[GA]GC[CT]^C
165 GGGCTC
BfaI 562 CTAG C^TAG
925 CTAG
BglI 1031 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1150 GCCAAGATGGC
BlpI 39 GCTCAGC GC^T[ACGT]AGC
1183 GCTGAGC
Bpu1102I 39 GCTCAGC GC^T[ACGT]AGC
1183 GCTGAGC
BsaHI 773 GGCGCC G[GA]^CG[CT]C
954 GACGCC
BseRI 25 GAGGAG GAGGAG(10/8)
28 GAGGAG
CelII 39 GCTCAGC GC^T[ACGT]AGC
1183 GCTGAGC
DraII 254 AGGACCT [GA]G^G[ACGT]CC[CT]
416 GGGACCT
EcoO109I 254 AGGACCT [GA]G^G[ACGT]CC[CT]
416 GGGACCT
HaeII 773 GGCGCC [GA]GCGC^[CT]
847 GGCGCT
Hin1I 773 GGCGCC G[GA]^CG[CT]C
954 GACGCC
HinfI 125 GAATC G^A[ACGT]TC
514 GAGTC
MaeI 562 CTAG C^TAG
925 CTAG
MspA1I 209 CAGCTG C[AC]G^C[GT]G
629 CAGCTG
PpuMI 254 AGGACCT [GA]G^G[AT]CC[CT]
416 GGGACCT
PvuII 209 CAGCTG CAG^CTG
629 CAGCTG
SmlI 657 CTCAAG C^T[CT][GA]AG
1078 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI Tsp45I TspRI XcmI XhoII