Restriction map for
pF1KB3774
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AclI AcsI AflII AhdI AlwNI
AocI Aor51HI ApoI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbrPI BciVI BclI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KspI MaeI
MamI MfeI MluI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp509I
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 319 GACGTC GACGT^C
Acc65I 824 GGTACC G^GTACC
AccI 1036 GTCTAC GT^[AC][GT]AC
AccIII 959 TCCGGA T^CCGGA
AflIII 884 ACGTGT A^C[GA][CT]GT
AgeI 941 ACCGGT A^CCGGT
Alw44I 973 GTGCAC G^TGCAC
ApaI 119 GGGCCC GGGCC^C
ApaLI 973 GTGCAC G^TGCAC
Asp718I 824 GGTACC G^GTACC
BbeI 543 GGCGCC GGCGC^C
BbsI 963 GAAGAC GAAGAC(2/6)
BbvCI 177 CCTCAGC CCTCAGC(-5/-2)
BcgI 231 CGATGCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 764 AGATCT A^GATCT
BpmI 382 CTGGAG CTGGAG(16/14)
BpuAI 963 GAAGAC GAAGAC(2/6)
BseAI 959 TCCGGA T^CCGGA
BspEI 959 TCCGGA T^CCGGA
BspHI 527 TCATGA T^CATGA
DraIII 136 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 67 CTCTTC CTCTTC(1/4)
EcoNI 177 CCTCAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1016 CCAAGG C^C[AT][AT]GG
KasI 543 GGCGCC G^GCGCC
KpnI 824 GGTACC GGTAC^C
Ksp632I 67 CTCTTC CTCTTC(1/4)
MroI 959 TCCGGA T^CCGGA
MspA1I 219 CCGCTG C[AC]G^C[GT]G
NarI 543 GGCGCC GG^CGCC
PflMI 84 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 941 ACCGGT A^CCGGT
PleI 112 GACTC GAGTC(4/5)
RcaI 527 TCATGA T^CATGA
SapI 66 GCTCTTC GCTCTTC(1/4)
SfoI 543 GGCGCC GGC^GCC
StyI 1016 CCAAGG C^C[AT][AT]GG
Tsp45I 360 GTCAC ^GT[GC]AC
Van91I 84 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 948 CGCG CG^CG
981 CGCG
AcyI 319 GACGTC G[GA]^CG[CT]C
543 GGCGCC
Asp700I 387 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
729 GAAGTCCTTC
AspHI 6 GTGCTC G[AT]GC[AT]^C
973 GTGCAC
BalI 92 TGGCCA TGG^CCA
1013 TGGCCA
BanII 119 GGGCCC G[GA]GC[CT]^C
486 GGGCTC
BsaHI 319 GACGTC G[GA]^CG[CT]C
543 GGCGCC
BsiHKAI 6 GTGCTC G[AT]GC[AT]^C
973 GTGCAC
BspMI 479 ACCTGC ACCTGC(4/8)
620 ACCTGC
BstUI 948 CGCG CG^CG
981 CGCG
EaeI 92 TGGCCA [CT]^GGCC[GA]
1013 TGGCCA
HgaI 132 GACGC GACGC(5/10)
979 GACGC
Hin1I 319 GACGTC G[GA]^CG[CT]C
543 GGCGCC
MaeIII 272 GTAAC ^GT[ACGT]AC
360 GTCAC
MluNI 92 TGGCCA TGG^CCA
1013 TGGCCA
MscI 92 TGGCCA TGG^CCA
1013 TGGCCA
MvnI 948 CGCG CG^CG
981 CGCG
NaeI 163 GCCGGC GCC^GGC
446 GCCGGC
NgoMIV 163 GCCGGC G^CCGGC
446 GCCGGC
NspI 864 GCATGT [GA]CATG^[CT]
1007 ACATGC
PpuMI 431 GGGACCC [GA]G^G[AT]CC[CT]
659 AGGTCCT
PstI 169 CTGCAG CTGCA^G
343 CTGCAG
SfcI 169 CTGCAG C^T[GA][CT]AG
343 CTGCAG
TfiI 537 GAATC G^A[AT]TC
710 GATTC
TspRI 442 CAGTG CA[GC]TG[ACGT][ACGT]^
725 CAGTG
XmnI 387 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
729 GAAGTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BanI BbvI BcnI BmyI
BsaJI BsaWI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MboI MboII MflI MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TseI
TthHB8I XcmI XhoII