Restriction map for
pF1KB3746
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DpnII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII Hin1I HindIII HpaI
KasI KpnI KspI MaeI MaeII
MboI MfeI MflI MluI MluNI
MroI MscI MslI MunI NaeI
NarI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI Sau3AI SbfI ScaI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Van91I XbaI XhoI
XhoII
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 861 CGCG CG^CG
ApaI 281 GGGCCC GGGCC^C
Asp700I 894 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 112 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 452 GGATAC GTATCC(6/5)
BpmI 634 CTCCAG CTGGAG(16/14)
BsaBI 624 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 292 CTCCTC GAGGAG(10/8)
BsgI 172 GTGCAG GTGCAG(16/14)
BsiEI 276 CGACCG CG[GA][CT]^CG
BsmAI 159 GAGAC GTCTC(1/5)
BstEII 117 GGTCACC G^GT[ACGT]ACC
BstPI 117 GGTCACC G^GT[ACGT]ACC
BstUI 861 CGCG CG^CG
CfoI 246 GCGC GCG^C
DraI 694 TTTAAA TTT^AAA
DraIII 974 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 759 CCATGG C^C[GA][CT]GG
EarI 133 GAAGAG CTCTTC(1/4)
EcoNI 807 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 117 GGTCACC G^GT[ACGT]ACC
FauI 260 CCCGC CCCGC(4/6)
HgaI 942 GACGC GACGC(5/10)
HhaI 246 GCGC GCG^C
HinP1I 246 GCGC G^CGC
HincII 298 GTCAAC GT[CT]^[GA]AC
HindII 298 GTCAAC GT[CT]^[GA]AC
Ksp632I 133 GAAGAG CTCTTC(1/4)
MamI 624 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 861 CGCG CG^CG
NcoI 759 CCATGG C^CATGG
PflFI 112 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 133 GAAGAGC GCTCTTC(1/4)
SexAI 113 ACCTGGT A^CC[AT]GGT
SmaI 128 CCCGGG CCC^GGG
SspI 690 AATATT AAT^ATT
TfiI 749 GATTC G^A[AT]TC
Tth111I 112 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 29 CCAGCCCTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 128 CCCGGG C^CCGGG
XmaI 128 CCCGGG C^CCGGG
XmnI 894 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 414 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
940 CAGACGCTG
AspHI 96 GTGCTC G[AT]GC[AT]^C
216 GAGCAC
AvaI 128 CCCGGG C^[CT]CG[GA]G
952 CCCGAG
BanII 257 GAGCCC G[GA]GC[CT]^C
281 GGGCCC
BcgI 174 GCAGGCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
218 GCACATCCCTCG
BsiHKAI 96 GTGCTC G[AT]GC[AT]^C
216 GAGCAC
BsoBI 128 CCCGGG C^[CT]CG[GA]G
952 CCCGAG
BstXI 442 CCAGACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
534 CCAGGTGAATGG
EcoT14I 759 CCATGG C^C[AT][AT]GG
812 CCAAGG
MseI 361 TTAA T^TAA
695 TTAA
MspA1I 309 CAGCTG C[AC]G^C[GT]G
1009 CAGCTG
NlaIV 281 GGGCCC GG[ACGT]^[ACGT]CC
383 GGTTCC
PleI 16 GACTC GAGTC(4/5)
985 GACTC
PstI 139 CTGCAG CTGCA^G
286 CTGCAG
PvuII 309 CAGCTG CAG^CTG
1009 CAGCTG
StyI 759 CCATGG C^C[AT][AT]GG
812 CCAAGG
Tru9I 361 TTAA T^TAA
695 TTAA
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI BbsI BbvI
BcnI BmyI BpuAI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboII MnlI MspI MvaI MwoI
NciI NlaIII RsaI Sau96I ScrFI
SfaNI SfcI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I