Restriction map for
pF1KB3737
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI AlwNI Aor51HI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbsI BcgI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BseAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MroI MseI
MunI MvnI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AfaI 109 GTAC GT^AC
AocI 484 CCTCAGG CC^T[ACGT]AGG
BbvCI 113 CCTCAGC CCTCAGC(-5/-2)
BciVI 546 GGATAC GTATCC(6/5)
BclI 1037 TGATCA T^GATCA
BcnI 1103 CCCGG CC^[GC]GG
BfaI 931 CTAG C^TAG
BglI 442 GCCTCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 871 AGATCT A^GATCT
BsaI 348 GAGACC GGTCTC(1/5)
BsaWI 866 ACCGGA [AT]^CCGG[AT]
BsiEI 651 CGGCCG CG[GA][CT]^CG
BsmAI 348 GAGAC GTCTC(1/5)
BsrDI 617 GCAATG GCAATG(2/0)
BstAPI 316 GCACTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 5 CCACGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 484 CCTCAGG CC^T[ACGT]AGG
CfoI 1095 GCGC GCG^C
DraIII 119 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 651 CGGCCG C^GGCCG
EarI 721 CTCTTC CTCTTC(1/4)
EclXI 651 CGGCCG C^GGCCG
Eco52I 651 CGGCCG C^GGCCG
Eco81I 484 CCTCAGG CC^T[ACGT]AGG
FbaI 1037 TGATCA T^GATCA
HaeII 1094 GGCGCT [GA]GCGC^[CT]
HhaI 1095 GCGC GCG^C
HinP1I 1095 GCGC G^CGC
HinfI 584 GACTC G^A[ACGT]TC
HphI 1071 TCACC GGTGA(8/7)
Ksp632I 721 CTCTTC CTCTTC(1/4)
MaeI 931 CTAG C^TAG
MboII 722 TCTTC GAAGA(8/7)
NaeI 940 GCCGGC GCC^GGC
NciI 1103 CCCGG CC^[GC]GG
NgoMIV 940 GCCGGC G^CCGGC
PflMI 607 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 584 GACTC GAGTC(4/5)
PstI 1065 CTGCAG CTGCA^G
PvuII 1179 CAGCTG CAG^CTG
RsaI 109 GTAC GT^AC
SexAI 1010 ACCTGGT A^CC[AT]GGT
SfcI 1065 CTGCAG C^T[GA][CT]AG
Van91I 607 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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Alw44I 142 GTGCAC G^TGCAC
533 GTGCAC
AlwI 333 GATCC GGATC(4/5)
1111 GATCC
ApaI 15 GGGCCC GGGCC^C
1026 GGGCCC
ApaLI 142 GTGCAC G^TGCAC
533 GTGCAC
Asp700I 1126 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1170 GAAGTCCTTC
BanI 821 GGCACC G^G[CT][GA]CC
1079 GGTGCC
BsgI 904 GTGCAG GTGCAG(16/14)
923 GTGCAG
BsrFI 649 ACCGGC [GA]^CCGG[CT]
940 GCCGGC
Cfr10I 649 ACCGGC [GA]^CCGG[CT]
940 GCCGGC
MslI 912 CATTGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
997 CATCTCCATG
NcoI 579 CCATGG C^CATGG
1083 CCATGG
PpuMI 74 AGGTCCC [GA]G^G[AT]CC[CT]
959 GGGACCT
Tsp45I 1045 GTGAC ^GT[GC]AC
1070 GTCAC
XcmI 1049 CCAGGGCAGCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1074 CCAGCGGTGCCATGG
XmnI 1126 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1170 GAAGTCCTTC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AspHI AvaII BalI
BanII BbvI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HpaII ItaI
MaeIII MboI MflI MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI StuI StyI TseI
TspRI XhoII