Restriction map for
pF1KB3634
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BmyI BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspDI BspEI BspHI BspLU11I
BspMI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CfoI Cfr10I Cfr13I ClaI
CpoI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MluI
MluNI MroI MscI MspA1I MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AseI 895 ATTAAT AT^TAAT
AsnI 895 ATTAAT AT^TAAT
Asp700I 300 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 640 GGTGCC G^G[CT][GA]CC
BcnI 19 CCGGG CC^[GC]GG
BfaI 97 CTAG C^TAG
BglII 195 AGATCT A^GATCT
BsrBI 8 GAGCGG CCGCTC(-3/-3)
BstNI 66 CCTGG CC^[AT]GG
BstXI 644 CCAAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 195 AGATCT [GA]^GATC[CT]
DraI 111 TTTAAA TTT^AAA
DsaI 504 CCGTGG C^C[GA][CT]GG
EarI 675 GAAGAG CTCTTC(1/4)
EcoRII 66 CCTGG ^CC[AT]GG
EcoT14I 138 CCTTGG C^C[AT][AT]GG
FauI 33 GCGGG CCCGC(4/6)
HinfI 91 GATTC G^A[ACGT]TC
Ksp632I 675 GAAGAG CTCTTC(1/4)
MaeI 97 CTAG C^TAG
MfeI 884 CAATTG C^AATTG
MflI 195 AGATCT [GA]^GATC[CT]
MunI 884 CAATTG C^AATTG
MvaI 66 CCTGG CC^[AT]GG
NciI 19 CCGGG CC^[GC]GG
PshBI 895 ATTAAT AT^TAAT
ScaI 568 AGTACT AGT^ACT
SmlI 379 CTTGAG C^T[CT][GA]AG
SphI 81 GCATGC GCATG^C
StuI 255 AGGCCT AGG^CCT
StyI 138 CCTTGG C^C[AT][AT]GG
TfiI 91 GATTC G^A[AT]TC
XcmI 852 CCAACATTTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 195 AGATCT [GA]^GATC[CT]
XmnI 300 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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BlpI 76 GCTGAGC GC^T[ACGT]AGC
235 GCTCAGC
BpmI 329 CTGGAG CTGGAG(16/14)
842 CTGGAG
Bpu1102I 76 GCTGAGC GC^T[ACGT]AGC
235 GCTCAGC
BsiYI 6 CCGAGCGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
30 CCGGCGGGCGG
BslI 6 CCGAGCGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
30 CCGGCGGGCGG
Bsp1407I 248 TGTACA T^GTACA
463 TGTACA
BsrDI 44 CATTGC GCAATG(2/0)
405 CATTGC
BsrGI 248 TGTACA T^GTACA
463 TGTACA
BsrI 548 CCAGT ACTGG(1/-1)
577 CCAGT
BssKI 19 CCGGG ^CC[ACGT]GG
66 CCTGG
BstAPI 448 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
730 GCAGTTGGTGC
CelII 76 GCTGAGC GC^T[ACGT]AGC
235 GCTCAGC
HaeIII 39 GGCC GG^CC
256 GGCC
HapII 19 CCGG C^CGG
30 CCGG
HpaII 19 CCGG C^CGG
30 CCGG
MaeIII 179 GTGAC ^GT[ACGT]AC
417 GTCAC
MboII 675 GAAGA GAAGA(8/7)
990 GAAGA
MspI 19 CCGG C^CGG
30 CCGG
ScrFI 19 CCGGG CC^[ACGT]GG
66 CCTGG
SspBI 248 TGTACA T^GTACA
463 TGTACA
TaqI 624 TCGA T^CGA
666 TCGA
Tsp45I 179 GTGAC ^GT[GC]AC
417 GTCAC
TspRI 467 CAGTG CA[GC]TG[ACGT][ACGT]^
578 CAGTG
TthHB8I 624 TCGA T^CGA
666 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AfaI AluI BbvI BsaJI
BstF5I Cac8I DdeI DpnII Fnu4HI
FokI HphI ItaI MboI MnlI
MseI MslI MwoI NdeII NlaIII
NlaIV NspI PstI RsaI Sau3AI
SfaNI SfcI Tru9I TseI Tsp509I