Restriction map for
pF1KB3614
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRII EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HaeIII
HhaI HinP1I HindIII HpaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI MslI MvaI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AciI 863 GCGG CCGC(-3/-1)
AcyI 500 GGCGTC G[GA]^CG[CT]C
AflIII 1178 ACATGT A^C[GA][CT]GT
AlwNI 293 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 886 GGACC G^G[AT]CC
AvrII 673 CCTAGG C^CTAGG
BanII 1088 GAGCCC G[GA]GC[CT]^C
BlnI 673 CCTAGG C^CTAGG
BmyI 1088 GAGCCC G[AGT]GC[ACT]^C
BsaHI 500 GGCGTC G[GA]^CG[CT]C
BsmFI 461 GGGAC GGGAC(10/14)
BsmI 641 GAATGC GAATGC(1/-1)
Bsp1286I 1088 GAGCCC G[AGT]GC[ACT]^C
BspHI 665 TCATGA T^CATGA
BspLU11I 1178 ACATGT A^CATGT
Cfr13I 886 GGACC G^G[ACGT]CC
DdeI 336 CTTAG C^T[ACGT]AG
DraI 1204 TTTAAA TTT^AAA
DsaI 496 CCATGG C^C[GA][CT]GG
Hin1I 500 GGCGTC G[GA]^CG[CT]C
HincII 430 GTCAAC GT[CT]^[GA]AC
HindII 430 GTCAAC GT[CT]^[GA]AC
MaeII 1220 ACGT A^CGT
MfeI 881 CAATTG C^AATTG
MspA1I 481 CAGCTG C[AC]G^C[GT]G
MunI 881 CAATTG C^AATTG
MwoI 65 GCGTTGTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 496 CCATGG C^CATGG
NlaIV 1087 GGAGCC GG[ACGT]^[ACGT]CC
PvuII 481 CAGCTG CAG^CTG
RcaI 665 TCATGA T^CATGA
Sau96I 886 GGACC G^G[ACGT]CC
SfcI 587 CTACAG C^T[GA][CT]AG
SmlI 104 CTCAAG C^T[CT][GA]AG
TaqI 252 TCGA T^CGA
TthHB8I 252 TCGA T^CGA
XbaI 45 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 15 enzymes) #
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BcnI 180 CCGGG CC^[GC]GG
476 CCGGG
BpmI 755 CTCCAG CTGGAG(16/14)
1085 CTGGAG
BsaWI 31 TCCGGT [AT]^CCGG[AT]
710 TCCGGT
BssKI 180 CCGGG ^CC[ACGT]GG
476 CCGGG
BstYI 583 GGATCT [GA]^GATC[CT]
1133 AGATCC
Cac8I 622 GCTTGC GC[ACGT]^[ACGT]GC
1143 GCTTGC
HgaI 562 GACGC GACGC(5/10)
501 GCGTC
MflI 583 GGATCT [GA]^GATC[CT]
1133 AGATCC
NciI 180 CCGGG CC^[GC]GG
476 CCGGG
NspI 938 ACATGC [GA]CATG^[CT]
1178 ACATGT
PleI 773 GACTC GAGTC(4/5)
1211 GAGTC
SapI 60 GAAGAGC GCTCTTC(1/4)
189 GCTCTTC
ScrFI 180 CCGGG CC^[ACGT]GG
476 CCGGG
SfaNI 619 GATGC GCATC(5/9)
707 GCATC
XhoII 583 GGATCT [GA]^GATC[CT]
1133 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BfaI BsaJI BsiYI BslI
BsrI BstF5I DpnII EarI EcoT14I
Fnu4HI FokI HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MspI
NdeII NlaIII RsaI Sau3AI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI