Restriction map for
pF1KB3604
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AvaI AviII AvrII
BalI BbeI BbrPI BcgI BciVI
BclI BfrI BlnI BsaAI BsaHI
BsaI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HindIII HpaI KasI KspI
MfeI MluI MluNI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 103 GTCGAC GT^[AC][GT]AC
AccII 645 CGCG CG^CG
AccIII 405 TCCGGA T^CCGGA
AcsI 112 GAATTT [GA]^AATT[CT]
ApaI 1016 GGGCCC GGGCC^C
ApoI 112 GAATTT [GA]^AATT[CT]
AspHI 1024 GAGCAC G[AT]GC[AT]^C
BamHI 70 GGATCC G^GATCC
BbvCI 1177 GCTGAGG CCTCAGC(-5/-2)
BfaI 158 CTAG C^TAG
BglI 1231 GCCGGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 671 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 671 GCTTAGC GC^T[ACGT]AGC
BsaBI 705 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 405 TCCGGA T^CCGGA
BsgI 999 CTGCAC GTGCAG(16/14)
BsiHKAI 1024 GAGCAC G[AT]GC[AT]^C
BsmBI 583 GAGACG CGTCTC(1/5)
BspEI 405 TCCGGA T^CCGGA
BspMI 1208 ACCTGC ACCTGC(4/8)
BstUI 645 CGCG CG^CG
BstXI 600 CCAGGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 671 GCTTAGC GC^T[ACGT]AGC
DrdI 77 GACACATTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 860 TGGCCG [CT]^GGCC[GA]
HaeII 23 AGCGCC [GA]GCGC^[CT]
MaeI 158 CTAG C^TAG
MamI 705 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 405 TCCGGA T^CCGGA
MvnI 645 CGCG CG^CG
NspI 368 GCATGT [GA]CATG^[CT]
PflMI 21 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 141 GACGGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 181 CAGCTG CAG^CTG
SalI 103 GTCGAC G^TCGAC
SexAI 251 ACCTGGT A^CC[AT]GGT
SspI 1468 AATATT AAT^ATT
StuI 1111 AGGCCT AGG^CCT
Van91I 21 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 885 CCAGTCCGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Acc65I 533 GGTACC G^GTACC
1274 GGTACC
AlwNI 22 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
203 CAGAGGCTG
Asp718I 533 GGTACC G^GTACC
1274 GGTACC
BanII 877 GAGCCC G[GA]GC[CT]^C
1016 GGGCCC
BglII 160 AGATCT A^GATCT
296 AGATCT
BpmI 1264 CTCCAG CTGGAG(16/14)
580 CTGGAG
BsmI 198 GAATGC GAATGC(1/-1)
275 GAATGC
HincII 103 GTCGAC GT[CT]^[GA]AC
376 GTCAAC
HindII 103 GTCGAC GT[CT]^[GA]AC
376 GTCAAC
HinfI 395 GAATC G^A[ACGT]TC
1339 GATTC
KpnI 533 GGTACC GGTAC^C
1274 GGTACC
MslI 474 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
522 CATCTTCATG
MspA1I 181 CAGCTG C[AC]G^C[GT]G
801 CCGCTG
PpuMI 1151 GGGACCC [GA]G^G[AT]CC[CT]
1205 AGGACCT
PstI 33 CTGCAG CTGCA^G
92 CTGCAG
SfaNI 626 GCATC GCATC(5/9)
910 GCATC
TfiI 395 GAATC G^A[AT]TC
1339 GATTC
Tsp45I 1403 GTGAC ^GT[GC]AC
1478 GTGAC
TspRI 1027 CACTG CA[GC]TG[ACGT][ACGT]^
1476 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BbsI BbvI BcnI BmyI
BpuAI BsaJI BsaWI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfcI StyI TaqI Tru9I
TseI Tsp509I TthHB8I XhoII