Restriction map for
pF1KB3590
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AvaI
AvaII AviII AvrII BamHI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BmyI BpmI BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CfoI ClaI CpoI DraI
DraII DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MnlI
MroI MseI MslI MunI MvnI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcyI 238 GGCGTC G[GA]^CG[CT]C
AfaI 99 GTAC GT^AC
AlwNI 270 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 3 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 147 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 189 TGGCCA TGG^CCA
BanI 343 GGTGCC G^G[CT][GA]CC
BfaI 158 CTAG C^TAG
BglII 5 AGATCT A^GATCT
BlpI 357 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 357 GCTGAGC GC^T[ACGT]AGC
BsaHI 238 GGCGTC G[GA]^CG[CT]C
BsiEI 305 CGGCCG CG[GA][CT]^CG
BsrI 16 CCAGT ACTGG(1/-1)
BssSI 178 CTCGTG CACGAG(-5/-1)
BstAPI 266 GCATCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 228 CATCC GGATG(2/0)
BstNI 129 CCAGG CC^[AT]GG
BstYI 5 AGATCT [GA]^GATC[CT]
CelII 357 GCTGAGC GC^T[ACGT]AGC
DpnII 6 GATC ^GATC
DsaI 260 CCATGG C^C[GA][CT]GG
EagI 305 CGGCCG C^GGCCG
EarI 87 GAAGAG CTCTTC(1/4)
EclXI 305 CGGCCG C^GGCCG
Eco52I 305 CGGCCG C^GGCCG
EcoRII 129 CCAGG ^CC[AT]GG
EcoT14I 260 CCATGG C^C[AT][AT]GG
FokI 228 CATCC GGATG(9/13)
Hin1I 238 GGCGTC G[GA]^CG[CT]C
HinfI 174 GAATC G^A[ACGT]TC
HphI 332 TCACC GGTGA(8/7)
Ksp632I 87 GAAGAG CTCTTC(1/4)
MaeI 158 CTAG C^TAG
MboI 6 GATC ^GATC
MflI 5 AGATCT [GA]^GATC[CT]
MluNI 189 TGGCCA TGG^CCA
MscI 189 TGGCCA TGG^CCA
MspA1I 270 CAGCTG C[AC]G^C[GT]G
MvaI 129 CCAGG CC^[AT]GG
NcoI 260 CCATGG C^CATGG
NdeII 6 GATC ^GATC
NlaIII 261 CATG CATG^
PflFI 147 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 270 CAGCTG CAG^CTG
RsaI 99 GTAC GT^AC
SapI 87 GAAGAGC GCTCTTC(1/4)
Sau3AI 6 GATC ^GATC
SfaNI 266 GCATC GCATC(5/9)
StyI 260 CCATGG C^C[AT][AT]GG
TfiI 174 GAATC G^A[AT]TC
Tsp509I 295 AATT ^AATT
Tth111I 147 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 5 AGATCT [GA]^GATC[CT]
XmnI 3 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BcnI 216 CCCGG CC^[GC]GG
347 CCGGG
BsaJI 215 CCCCGG C^C[ACGT][ACGT]GG
260 CCATGG
BsrFI 163 GCCGGC [GA]^CCGG[CT]
369 GCCGGC
Cfr10I 163 GCCGGC [GA]^CCGG[CT]
369 GCCGGC
Cfr13I 372 GGCCC G^G[ACGT]CC
388 GGCCC
DdeI 199 CTGAG C^T[ACGT]AG
358 CTGAG
EaeI 189 TGGCCA [CT]^GGCC[GA]
305 CGGCCG
FauI 314 GCGGG CCCGC(4/6)
321 GCGGG
HgaI 239 GCGTC GACGC(5/10)
329 GCGTC
NaeI 163 GCCGGC GCC^GGC
369 GCCGGC
NciI 216 CCCGG CC^[GC]GG
347 CCGGG
NgoMIV 163 GCCGGC G^CCGGC
369 GCCGGC
NlaIV 343 GGTGCC GG[ACGT]^[ACGT]CC
388 GGCCCC
Sau96I 372 GGCCC G^G[ACGT]CC
388 GGCCC
TspRI 17 CAGTG CA[GC]TG[ACGT][ACGT]^
300 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI AluI BbvI BsiYI BslI
BssKI Cac8I Fnu4HI HaeIII HapII
HpaII ItaI MaeIII MboII MspI
MwoI ScrFI TseI Tsp45I