Restriction map for
pF1KB3502
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaHI BsaI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRII EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI Ksp632I
KspI MaeI MfeI MflI MluI
MluNI MroI MscI MslI MunI
MvaI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 49 AAATTC [GA]^AATT[CT]
AfaI 153 GTAC GT^AC
AlwI 235 GATCC GGATC(4/5)
AlwNI 16 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 49 AAATTC [GA]^AATT[CT]
AseI 199 ATTAAT AT^TAAT
AsnI 199 ATTAAT AT^TAAT
AspHI 43 GAGCTC G[AT]GC[AT]^C
AvaI 28 CTCGAG C^[CT]CG[GA]G
BanII 43 GAGCTC G[GA]GC[CT]^C
BcnI 665 CCCGG CC^[GC]GG
BmyI 43 GAGCTC G[AGT]GC[ACT]^C
BsaAI 146 CACGTA [CT]AC^GT[GA]
BsaBI 229 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 54 CCGAGG C^C[ACGT][ACGT]GG
BsaWI 330 ACCGGA [AT]^CCGG[AT]
BseRI 553 GAGGAG GAGGAG(10/8)
BsgI 604 GTGCAG GTGCAG(16/14)
BsiHKAI 43 GAGCTC G[AT]GC[AT]^C
BsmAI 465 GTCTC GTCTC(1/5)
BsmFI 59 GTCCC GGGAC(10/14)
BsoBI 28 CTCGAG C^[CT]CG[GA]G
Bsp1286I 43 GAGCTC G[AGT]GC[ACT]^C
BspDI 181 ATCGAT AT^CGAT
BspMI 15 GCAGGT ACCTGC(4/8)
BsrI 640 ACTGG ACTGG(1/-1)
BssKI 665 CCCGG ^CC[ACGT]GG
BssSI 40 CACGAG CACGAG(-5/-1)
Cac8I 375 GCCAGC GC[ACGT]^[ACGT]GC
ClaI 181 ATCGAT AT^CGAT
DpnII 235 GATC ^GATC
HindIII 91 AAGCTT A^AGCTT
MaeII 147 ACGT A^CGT
MamI 229 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 235 GATC ^GATC
MspA1I 171 CAGCGG C[AC]G^C[GT]G
MwoI 36 GCAGCACGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 665 CCCGG CC^[GC]GG
NdeII 235 GATC ^GATC
NlaIII 397 CATG CATG^
PaeR7I 28 CTCGAG C^TCGAG
PshBI 199 ATTAAT AT^TAAT
RsaI 153 GTAC GT^AC
SacI 43 GAGCTC GAGCT^C
Sau3AI 235 GATC ^GATC
ScrFI 665 CCCGG CC^[ACGT]GG
SfcI 137 CTACAG C^T[GA][CT]AG
SmlI 28 CTCGAG C^T[CT][GA]AG
StuI 654 AGGCCT AGG^CCT
Tsp45I 76 GTCAC ^GT[GC]AC
TspRI 602 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 28 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AluI 44 AGCT AG^CT
92 AGCT
AvaII 18 GGTCC G^G[AT]CC
58 GGTCC
BbvI 36 GCAGC GCAGC(8/12)
525 GCTGC
BstF5I 483 GGATG GGATG(2/0)
590 GGATG
FokI 483 GGATG GGATG(9/13)
590 GGATG
HapII 331 CCGG C^CGG
666 CCGG
HinfI 176 GAATC G^A[ACGT]TC
346 GATTC
HpaII 331 CCGG C^CGG
666 CCGG
MaeIII 76 GTCAC ^GT[ACGT]AC
216 GTTAC
MspI 331 CCGG C^CGG
666 CCGG
NlaIV 58 GGTCCC GG[ACGT]^[ACGT]CC
190 GGGGCC
PpuMI 17 AGGTCCT [GA]G^G[AT]CC[CT]
57 AGGTCCC
SfaNI 184 GATGC GCATC(5/9)
433 GCATC
TfiI 176 GAATC G^A[AT]TC
346 GATTC
TseI 36 GCAGC G^C[AT]GC
525 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI BbsI BpuAI BsiYI BslI
Cfr13I DdeI DraII EcoO109I Fnu4HI
HaeIII HphI ItaI MboII MnlI
MseI Sau96I TaqI Tru9I Tsp509I
TthHB8I