Restriction map for
pF1KB3489
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BciVI BfaI BfrI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BspDI
BspEI BspHI BspLU11I BsrFI BssHII
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoRI EcoRV
EcoT22I FauI FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MfeI MluI MroI MspA1I
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TfiI Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 625 GTCTAC GT^[AC][GT]AC
AlwI 500 GGATC GGATC(4/5)
AseI 241 ATTAAT AT^TAAT
AsnI 241 ATTAAT AT^TAAT
AvaI 91 CCCGGG C^[CT]CG[GA]G
AvaII 337 GGTCC G^G[AT]CC
BalI 749 TGGCCA TGG^CCA
BanII 868 GGGCTC G[GA]GC[CT]^C
BcgI 140 GCATTGCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 467 TGATCA T^GATCA
BglI 488 GCCGGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 393 AGATCT A^GATCT
BmyI 868 GGGCTC G[AGT]GC[ACT]^C
BsaI 718 GAGACC GGTCTC(1/5)
BsgI 362 GTGCAG GTGCAG(16/14)
BsoBI 91 CCCGGG C^[CT]CG[GA]G
Bsp1286I 868 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 47 TGTACA T^GTACA
BspMI 876 GCAGGT ACCTGC(4/8)
BsrBI 61 CCGCTC CCGCTC(-3/-3)
BsrDI 141 CATTGC GCAATG(2/0)
BsrGI 47 TGTACA T^GTACA
BssSI 25 CTCGTG CACGAG(-5/-1)
BstEII 882 GGTCACC G^GT[ACGT]ACC
BstPI 882 GGTCACC G^GT[ACGT]ACC
BstYI 393 AGATCT [GA]^GATC[CT]
DsaI 887 CCATGG C^C[GA][CT]GG
EaeI 749 TGGCCA [CT]^GGCC[GA]
EcoNI 410 CCTTTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 882 GGTCACC G^GT[ACGT]ACC
FbaI 467 TGATCA T^GATCA
HaeII 485 AGCGCC [GA]GCGC^[CT]
HindIII 593 AAGCTT A^AGCTT
HinfI 702 GACTC G^A[ACGT]TC
MflI 393 AGATCT [GA]^GATC[CT]
MluNI 749 TGGCCA TGG^CCA
MscI 749 TGGCCA TGG^CCA
NcoI 887 CCATGG C^CATGG
PflMI 873 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 702 GACTC GAGTC(4/5)
PshBI 241 ATTAAT AT^TAAT
PstI 208 CTGCAG CTGCA^G
SfcI 208 CTGCAG C^T[GA][CT]AG
SmaI 91 CCCGGG CCC^GGG
SmlI 284 CTCAAG C^T[CT][GA]AG
SspBI 47 TGTACA T^GTACA
Tsp45I 883 GTCAC ^GT[GC]AC
Van91I 873 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 575 CCATAAGGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 393 AGATCT [GA]^GATC[CT]
XmaCI 91 CCCGGG C^CCGGG
XmaI 91 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AcsI 385 AAATTT [GA]^AATT[CT]
783 AAATTT
ApoI 385 AAATTT [GA]^AATT[CT]
783 AAATTT
BpmI 845 CTCCAG CTGGAG(16/14)
871 CTCCAG
BstF5I 563 CATCC GGATG(2/0)
839 GGATG
Cac8I 9 GCTGGC GC[ACGT]^[ACGT]GC
210 GCAGGC
CfoI 97 GCGC GCG^C
486 GCGC
DdeI 804 CTCAG C^T[ACGT]AG
863 CTGAG
FokI 563 CATCC GGATG(9/13)
839 GGATG
HhaI 97 GCGC GCG^C
486 GCGC
HinP1I 97 GCGC G^CGC
486 GCGC
MboII 391 GAAGA GAAGA(8/7)
676 GAAGA
TaqI 149 TCGA T^CGA
705 TCGA
TthHB8I 149 TCGA T^CGA
705 TCGA
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI BbvI BcnI
BsaJI BsiYI BslI BsmAI BsrI
BssKI BstNI Cfr13I DpnII EcoRII
EcoT14I Fnu4HI HaeIII HapII HpaII
HphI ItaI MaeIII MboI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI Tru9I TseI Tsp509I TspRI