Restriction map for
pF1KB3488
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccIII AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BciVI BfrI BglI BglII BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1286I Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I CelII CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PvuI RcaI RsaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AclI 610 AACGTT AA^CGTT
AcsI 342 GAATTT [GA]^AATT[CT]
AlwNI 920 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 185 CCTCAGG CC^T[ACGT]AGG
ApoI 342 GAATTT [GA]^AATT[CT]
AseI 478 ATTAAT AT^TAAT
AsnI 478 ATTAAT AT^TAAT
AvrII 573 CCTAGG C^CTAGG
BbvCI 993 GCTGAGG CCTCAGC(-5/-2)
BcgI 228 CGAGAATCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 574 CTAG C^TAG
BlnI 573 CCTAGG C^CTAGG
BsgI 637 CTGCAC GTGCAG(16/14)
BsmAI 298 GAGAC GTCTC(1/5)
BsmFI 662 GTCCC GGGAC(10/14)
BspDI 695 ATCGAT AT^CGAT
BspMI 760 GCAGGT ACCTGC(4/8)
BsrFI 519 ACCGGC [GA]^CCGG[CT]
BstAPI 150 GCAGGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 98 TTCGAA TT^CGAA
BstF5I 456 GGATG GGATG(2/0)
BstXI 941 CCATGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 428 GGATCT [GA]^GATC[CT]
Bsu36I 185 CCTCAGG CC^T[ACGT]AGG
Cac8I 48 GCCCGC GC[ACGT]^[ACGT]GC
Cfr10I 519 ACCGGC [GA]^CCGG[CT]
ClaI 695 ATCGAT AT^CGAT
DsaI 529 CCATGG C^C[GA][CT]GG
EaeI 199 CGGCCA [CT]^GGCC[GA]
Eco81I 185 CCTCAGG CC^T[ACGT]AGG
FauI 49 CCCGC CCCGC(4/6)
FokI 456 GGATG GGATG(9/13)
HphI 670 GGTGA GGTGA(8/7)
MaeI 574 CTAG C^TAG
MflI 428 GGATCT [GA]^GATC[CT]
NcoI 529 CCATGG C^CATGG
NlaIV 937 GGCCCC GG[ACGT]^[ACGT]CC
NspV 98 TTCGAA TT^CGAA
PflMI 919 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 890 GAGTC GAGTC(4/5)
PshBI 478 ATTAAT AT^TAAT
Psp1406I 610 AACGTT AA^CGTT
SapI 1003 GAAGAGC GCTCTTC(1/4)
SfaNI 673 GATGC GCATC(5/9)
SfuI 98 TTCGAA TT^CGAA
Tsp45I 242 GTCAC ^GT[GC]AC
Van91I 919 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 428 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 51 CGCG CG^CG
90 CGCG
BclI 776 TGATCA T^GATCA
791 TGATCA
BcnI 35 CCCGG CC^[GC]GG
664 CCCGG
BsmI 617 GAATGC GAATGC(1/-1)
563 GCATTC
BstUI 51 CGCG CG^CG
90 CGCG
CfoI 52 GCGC GCG^C
652 GCGC
Cfr13I 135 GGCCC G^G[ACGT]CC
937 GGCCC
EcoT14I 529 CCATGG C^C[AT][AT]GG
573 CCTAGG
FbaI 776 TGATCA T^GATCA
791 TGATCA
HhaI 52 GCGC GCG^C
652 GCGC
HinP1I 52 GCGC G^CGC
652 GCGC
MaeII 611 ACGT A^CGT
660 ACGT
MseI 346 TTAA T^TAA
479 TTAA
MspA1I 57 CAGCTG C[AC]G^C[GT]G
272 CAGCTG
MvnI 51 CGCG CG^CG
90 CGCG
NciI 35 CCCGG CC^[GC]GG
664 CCCGG
PvuII 57 CAGCTG CAG^CTG
272 CAGCTG
Sau96I 135 GGCCC G^G[ACGT]CC
937 GGCCC
StyI 529 CCATGG C^C[AT][AT]GG
573 CCTAGG
TaqI 99 TCGA T^CGA
696 TCGA
TfiI 231 GAATC G^A[AT]TC
287 GAATC
Tru9I 346 TTAA T^TAA
479 TTAA
TthHB8I 99 TCGA T^CGA
696 TCGA
XcmI 529 CCATGGGGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
854 CCAAACCCAAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI AlwI BbvI BpmI
BsaJI BsiYI BslI BsrDI BsrI
BssKI BstNI DdeI DpnII EarI
EcoRII Fnu4HI HaeIII HapII HinfI
HpaII ItaI Ksp632I MaeIII MboI
MboII MnlI MslI MspI MvaI
MwoI NdeII NlaIII PstI Sau3AI
ScrFI SfcI TseI Tsp509I TspRI