##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccIII AciI AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BstXI BstYI
Bsu36I Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRV FauI FbaI
FseI FspI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MroI
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 1120 CGCG CG^CG
AcsI 248 GAATTC [GA]^AATT[CT]
AlwI 177 GGATC GGATC(4/5)
ApoI 248 GAATTC [GA]^AATT[CT]
Asp700I 969 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 202 GAAGAC GAAGAC(2/6)
BfaI 1003 CTAG C^TAG
BglI 754 GCCGAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 679 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 679 GCTGAGC GC^T[ACGT]AGC
BpuAI 202 GAAGAC GAAGAC(2/6)
BsaAI 1018 TACGTG [CT]AC^GT[GA]
BsaWI 434 ACCGGA [AT]^CCGG[AT]
BseRI 341 CTCCTC GAGGAG(10/8)
BsmI 998 GCATTC GAATGC(1/-1)
BspMI 808 ACCTGC ACCTGC(4/8)
BssSI 40 CACGAG CACGAG(-5/-1)
BstBI 94 TTCGAA TT^CGAA
BstEII 736 GGTGACC G^GT[ACGT]ACC
BstPI 736 GGTGACC G^GT[ACGT]ACC
BstUI 1120 CGCG CG^CG
CelII 679 GCTGAGC GC^T[ACGT]AGC
CfoI 1119 GCGC GCG^C
DpnII 178 GATC ^GATC
DraI 66 TTTAAA TTT^AAA
DsaI 662 CCATGG C^C[GA][CT]GG
EcoO65I 736 GGTGACC G^GT[ACGT]ACC
EcoRI 248 GAATTC G^AATTC
EcoT22I 1148 ATGCAT ATGCA^T
HapII 435 CCGG C^CGG
HhaI 1119 GCGC GCG^C
HinP1I 1119 GCGC G^CGC
HincII 826 GTTGAC GT[CT]^[GA]AC
HindII 826 GTTGAC GT[CT]^[GA]AC
HpaII 435 CCGG C^CGG
MaeI 1003 CTAG C^TAG
MaeII 1019 ACGT A^CGT
MboI 178 GATC ^GATC
MseI 67 TTAA T^TAA
MspI 435 CCGG C^CGG
MvnI 1120 CGCG CG^CG
NcoI 662 CCATGG C^CATGG
NdeII 178 GATC ^GATC
NsiI 1148 ATGCAT ATGCA^T
NspV 94 TTCGAA TT^CGAA
PpuMI 476 AGGACCC [GA]G^G[AT]CC[CT]
SapI 145 GAAGAGC GCTCTTC(1/4)
Sau3AI 178 GATC ^GATC
SfuI 94 TTCGAA TT^CGAA
SmlI 162 CTTGAG C^T[CT][GA]AG
SphI 1146 GCATGC GCATG^C
SspI 698 AATATT AAT^ATT
Tru9I 67 TTAA T^TAA
XmnI 969 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 213 GTATAC GT^[AC][GT]AC
936 GTATAC
AfaI 375 GTAC GT^AC
432 GTAC
AlwNI 290 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
804 CAGCACCTG
AspHI 682 GAGCAC G[AT]GC[AT]^C
1170 GTGCTC
AvaII 3 GGACC G^G[AT]CC
477 GGACC
BalI 138 TGGCCA TGG^CCA
365 TGGCCA
BanI 782 GGCACC G^G[CT][GA]CC
962 GGCACC
BanII 56 GAGCCC G[GA]GC[CT]^C
338 GGGCTC
BpmI 108 CTGGAG CTGGAG(16/14)
626 CTGGAG
BsiHKAI 682 GAGCAC G[AT]GC[AT]^C
1170 GTGCTC
BsmAI 310 GTCTC GTCTC(1/5)
501 GTCTC
Bst1107I 213 GTATAC GTA^TAC
936 GTATAC
BstZ17I 213 GTATAC GTA^TAC
936 GTATAC
DraII 476 AGGACCC [GA]G^G[ACGT]CC[CT]
603 AGGCCCC
DraIII 1069 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1155 CACAGAGTG
EaeI 138 TGGCCA [CT]^GGCC[GA]
365 TGGCCA
EcoO109I 476 AGGACCC [GA]G^G[ACGT]CC[CT]
603 AGGCCCC
HphI 513 GGTGA GGTGA(8/7)
736 GGTGA
MluNI 138 TGGCCA TGG^CCA
365 TGGCCA
MscI 138 TGGCCA TGG^CCA
365 TGGCCA
MslI 378 CATGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
874 CATTACTGTG
MspA1I 445 CAGCTG C[AC]G^C[GT]G
1140 CAGCTG
NspI 377 ACATGC [GA]CATG^[CT]
1146 GCATGC
PstI 801 CTGCAG CTGCA^G
1179 CTGCAG
PvuII 445 CAGCTG CAG^CTG
1140 CAGCTG
RsaI 375 GTAC GT^AC
432 GTAC
SfcI 801 CTGCAG C^T[GA][CT]AG
1179 CTGCAG
TspRI 653 CAGTG CA[GC]TG[ACGT][ACGT]^
1238 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AluI BbvI BmyI BsaJI BsgI
BsiYI BslI Bsp1286I BsrDI BsrI
BssKI BstAPI BstF5I BstNI Cac8I
Cfr13I DdeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeIII MboII MnlI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
SfaNI StyI TaqI TfiI TseI
Tsp45I Tsp509I TthHB8I