Restriction map for pF1KB3456
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvrII       BamHI       BanI        BbeI        BbvCI       
BcgI        BciVI       BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BsaI        
BseAI       BsgI        BsiEI       BsiWI       BsmFI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoT22I     FauI        FseI        
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MflI        MluI        MroI        MunI        
MvnI        NarI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Asp700I       718  GAAACTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         353  GAGCTC               G[AT]GC[AT]^C
AvaI          845  CCCGGG               C^[CT]CG[GA]G
AvaII          78  GGTCC                G^G[AT]CC
AviII         560  TGCGCA               TGC^GCA
BalI         1034  TGGCCA               TGG^CCA
BbrPI         541  CACGTG               CAC^GTG
BbsI          127  GAAGAC               GAAGAC(2/6)
BclI          413  TGATCA               T^GATCA
BpuAI         127  GAAGAC               GAAGAC(2/6)
BsaAI         541  CACGTG               [CT]AC^GT[GA]
BsiHKAI       353  GAGCTC               G[AT]GC[AT]^C
BsmBI         106  GAGACG               CGTCTC(1/5)
BsmI          433  GCATTC               GAATGC(1/-1)
BsoBI         845  CCCGGG               C^[CT]CG[GA]G
BspMI         283  ACCTGC               ACCTGC(4/8)
BsrBI         375  CCGCTC               CCGCTC(-3/-3)
BsrDI         417  CATTGC               GCAATG(2/0)
BsrFI           3  GCCGGC               [GA]^CCGG[CT]
BssSI         461  CTCGTG               CACGAG(-5/-1)
Cfr10I          3  GCCGGC               [GA]^CCGG[CT]
DdeI          620  CTGAG                C^T[ACGT]AG
DraII          77  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EarI          808  CTCTTC               CTCTTC(1/4)
EcoO109I       77  GGGTCCT              [GA]G^G[ACGT]CC[CT]
FbaI          413  TGATCA               T^GATCA
FspI          560  TGCGCA               TGC^GCA
HaeII          63  AGCGCC               [GA]GCGC^[CT]
HinfI         276  GATTC                G^A[ACGT]TC
Ksp632I       808  CTCTTC               CTCTTC(1/4)
MaeIII        464  GTGAC                ^GT[ACGT]AC
MluNI        1034  TGGCCA               TGG^CCA
MscI         1034  TGGCCA               TGG^CCA
NaeI            3  GCCGGC               GCC^GGC
NgoMIV          3  GCCGGC               G^CCGGC
NspI          498  ACATGC               [GA]CATG^[CT]
PmaCI         541  CACGTG               CAC^GTG
PmlI          541  CACGTG               CAC^GTG
PpuMI          77  GGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         572  CAGCTG               CAG^CTG
SacI          353  GAGCTC               GAGCT^C
SfaNI         111  GATGC                GCATC(5/9)
SmaI          845  CCCGGG               CCC^GGG
TfiI          276  GATTC                G^A[AT]TC
Tsp45I        464  GTGAC                ^GT[GC]AC
Tsp509I       514  AATT                 ^AATT
XmaCI         845  CCCGGG               C^CCGGG
XmaI          845  CCCGGG               C^CCGGG
XmnI          718  GAAACTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AlwI          505  GATCC                GGATC(4/5)
              266  GGATC               
BcnI          845  CCCGG                CC^[GC]GG
              846  CCGGG               
BglI          396  GCCCCTACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1027  GCCATCTTGGC         
BpmI           72  CTCCAG               CTGGAG(16/14)
              216  CTGGAG              
BsaWI        1004  ACCGGA               [AT]^CCGG[AT]
             1051  ACCGGA              
BseRI          92  GAGGAG               GAGGAG(10/8)
              642  GAGGAG              
BsrI          627  ACTGG                ACTGG(1/-1)
              929  CCAGT               
BstF5I        228  CATCC                GGATG(2/0)
              681  CATCC               
BstXI         422  CCAACACAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              779  CCACCTGGCTGG        
CfoI           64  GCGC                 GCG^C
              561  GCGC                
EaeI          980  TGGCCG               [CT]^GGCC[GA]
             1034  TGGCCA              
EcoRV          25  GATATC               GAT^ATC
              815  GATATC              
EcoT14I       200  CCAAGG               C^C[AT][AT]GG
             1019  CCAAGG              
FokI          228  CATCC                GGATG(9/13)
              681  CATCC               
HhaI           64  GCGC                 GCG^C
              561  GCGC                
HinP1I         64  GCGC                 G^CGC
              561  GCGC                
MaeII         522  ACGT                 A^CGT
              542  ACGT                
MseI          773  TTAA                 T^TAA
             1043  TTAA                
MslI          228  CATCCTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              690  CACGCTTGTG          
MspA1I        572  CAGCTG               C[AC]G^C[GT]G
              939  CCGCTG              
NciI          845  CCCGG                CC^[GC]GG
              846  CCGGG               
NlaIII        499  CATG                 CATG^
              978  CATG                
PstI           16  CTGCAG               CTGCA^G
               82  CTGCAG              
SfcI           16  CTGCAG               C^T[GA][CT]AG
               82  CTGCAG              
StyI          200  CCAAGG               C^C[AT][AT]GG
             1019  CCAAGG              
Tru9I         773  TTAA                 T^TAA
             1043  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AfaI        AluI        BanII       BbvI        
BfaI        BmyI        BsaJI       BsiYI       BslI        
BsmAI       Bsp1286I    BssKI       BstNI       Cac8I       
Cfr13I      DpnII       EcoRII      Fnu4HI      HaeIII      
HapII       HpaII       HphI        ItaI        MaeI        
MboI        MboII       MnlI        MspI        MvaI        
MwoI        NdeII       NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       TseI        TspRI       
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