################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BanI BbeI BbvCI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FseI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Asp700I 718 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 353 GAGCTC G[AT]GC[AT]^C AvaI 845 CCCGGG C^[CT]CG[GA]G AvaII 78 GGTCC G^G[AT]CC AviII 560 TGCGCA TGC^GCA BalI 1034 TGGCCA TGG^CCA BbrPI 541 CACGTG CAC^GTG BbsI 127 GAAGAC GAAGAC(2/6) BclI 413 TGATCA T^GATCA BpuAI 127 GAAGAC GAAGAC(2/6) BsaAI 541 CACGTG [CT]AC^GT[GA] BsiHKAI 353 GAGCTC G[AT]GC[AT]^C BsmBI 106 GAGACG CGTCTC(1/5) BsmI 433 GCATTC GAATGC(1/-1) BsoBI 845 CCCGGG C^[CT]CG[GA]G BspMI 283 ACCTGC ACCTGC(4/8) BsrBI 375 CCGCTC CCGCTC(-3/-3) BsrDI 417 CATTGC GCAATG(2/0) BsrFI 3 GCCGGC [GA]^CCGG[CT] BssSI 461 CTCGTG CACGAG(-5/-1) Cfr10I 3 GCCGGC [GA]^CCGG[CT] DdeI 620 CTGAG C^T[ACGT]AG DraII 77 GGGTCCT [GA]G^G[ACGT]CC[CT] EarI 808 CTCTTC CTCTTC(1/4) EcoO109I 77 GGGTCCT [GA]G^G[ACGT]CC[CT] FbaI 413 TGATCA T^GATCA FspI 560 TGCGCA TGC^GCA HaeII 63 AGCGCC [GA]GCGC^[CT] HinfI 276 GATTC G^A[ACGT]TC Ksp632I 808 CTCTTC CTCTTC(1/4) MaeIII 464 GTGAC ^GT[ACGT]AC MluNI 1034 TGGCCA TGG^CCA MscI 1034 TGGCCA TGG^CCA NaeI 3 GCCGGC GCC^GGC NgoMIV 3 GCCGGC G^CCGGC NspI 498 ACATGC [GA]CATG^[CT] PmaCI 541 CACGTG CAC^GTG PmlI 541 CACGTG CAC^GTG PpuMI 77 GGGTCCT [GA]G^G[AT]CC[CT] PvuII 572 CAGCTG CAG^CTG SacI 353 GAGCTC GAGCT^C SfaNI 111 GATGC GCATC(5/9) SmaI 845 CCCGGG CCC^GGG TfiI 276 GATTC G^A[AT]TC Tsp45I 464 GTGAC ^GT[GC]AC Tsp509I 514 AATT ^AATT XmaCI 845 CCCGGG C^CCGGG XmaI 845 CCCGGG C^CCGGG XmnI 718 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwI 505 GATCC GGATC(4/5) 266 GGATC BcnI 845 CCCGG CC^[GC]GG 846 CCGGG BglI 396 GCCCCTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1027 GCCATCTTGGC BpmI 72 CTCCAG CTGGAG(16/14) 216 CTGGAG BsaWI 1004 ACCGGA [AT]^CCGG[AT] 1051 ACCGGA BseRI 92 GAGGAG GAGGAG(10/8) 642 GAGGAG BsrI 627 ACTGG ACTGG(1/-1) 929 CCAGT BstF5I 228 CATCC GGATG(2/0) 681 CATCC BstXI 422 CCAACACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 779 CCACCTGGCTGG CfoI 64 GCGC GCG^C 561 GCGC EaeI 980 TGGCCG [CT]^GGCC[GA] 1034 TGGCCA EcoRV 25 GATATC GAT^ATC 815 GATATC EcoT14I 200 CCAAGG C^C[AT][AT]GG 1019 CCAAGG FokI 228 CATCC GGATG(9/13) 681 CATCC HhaI 64 GCGC GCG^C 561 GCGC HinP1I 64 GCGC G^CGC 561 GCGC MaeII 522 ACGT A^CGT 542 ACGT MseI 773 TTAA T^TAA 1043 TTAA MslI 228 CATCCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 690 CACGCTTGTG MspA1I 572 CAGCTG C[AC]G^C[GT]G 939 CCGCTG NciI 845 CCCGG CC^[GC]GG 846 CCGGG NlaIII 499 CATG CATG^ 978 CATG PstI 16 CTGCAG CTGCA^G 82 CTGCAG SfcI 16 CTGCAG C^T[GA][CT]AG 82 CTGCAG StyI 200 CCAAGG C^C[AT][AT]GG 1019 CCAAGG Tru9I 773 TTAA T^TAA 1043 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AfaI AluI BanII BbvI BfaI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstNI Cac8I Cfr13I DpnII EcoRII Fnu4HI HaeIII HapII HpaII HphI ItaI MaeI MboI MboII MnlI MspI MvaI MwoI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI TseI TspRI