Restriction map for
pF1KB3456
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BamHI BanI BbeI BbvCI
BcgI BciVI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BseAI BsgI BsiEI BsiWI BsmFI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FauI FseI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MroI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 49 enzymes) #
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Asp700I 718 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 353 GAGCTC G[AT]GC[AT]^C
AvaI 845 CCCGGG C^[CT]CG[GA]G
AvaII 78 GGTCC G^G[AT]CC
AviII 560 TGCGCA TGC^GCA
BalI 1034 TGGCCA TGG^CCA
BbrPI 541 CACGTG CAC^GTG
BbsI 127 GAAGAC GAAGAC(2/6)
BclI 413 TGATCA T^GATCA
BpuAI 127 GAAGAC GAAGAC(2/6)
BsaAI 541 CACGTG [CT]AC^GT[GA]
BsiHKAI 353 GAGCTC G[AT]GC[AT]^C
BsmBI 106 GAGACG CGTCTC(1/5)
BsmI 433 GCATTC GAATGC(1/-1)
BsoBI 845 CCCGGG C^[CT]CG[GA]G
BspMI 283 ACCTGC ACCTGC(4/8)
BsrBI 375 CCGCTC CCGCTC(-3/-3)
BsrDI 417 CATTGC GCAATG(2/0)
BsrFI 3 GCCGGC [GA]^CCGG[CT]
BssSI 461 CTCGTG CACGAG(-5/-1)
Cfr10I 3 GCCGGC [GA]^CCGG[CT]
DdeI 620 CTGAG C^T[ACGT]AG
DraII 77 GGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 808 CTCTTC CTCTTC(1/4)
EcoO109I 77 GGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 413 TGATCA T^GATCA
FspI 560 TGCGCA TGC^GCA
HaeII 63 AGCGCC [GA]GCGC^[CT]
HinfI 276 GATTC G^A[ACGT]TC
Ksp632I 808 CTCTTC CTCTTC(1/4)
MaeIII 464 GTGAC ^GT[ACGT]AC
MluNI 1034 TGGCCA TGG^CCA
MscI 1034 TGGCCA TGG^CCA
NaeI 3 GCCGGC GCC^GGC
NgoMIV 3 GCCGGC G^CCGGC
NspI 498 ACATGC [GA]CATG^[CT]
PmaCI 541 CACGTG CAC^GTG
PmlI 541 CACGTG CAC^GTG
PpuMI 77 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 572 CAGCTG CAG^CTG
SacI 353 GAGCTC GAGCT^C
SfaNI 111 GATGC GCATC(5/9)
SmaI 845 CCCGGG CCC^GGG
TfiI 276 GATTC G^A[AT]TC
Tsp45I 464 GTGAC ^GT[GC]AC
Tsp509I 514 AATT ^AATT
XmaCI 845 CCCGGG C^CCGGG
XmaI 845 CCCGGG C^CCGGG
XmnI 718 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 505 GATCC GGATC(4/5)
266 GGATC
BcnI 845 CCCGG CC^[GC]GG
846 CCGGG
BglI 396 GCCCCTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1027 GCCATCTTGGC
BpmI 72 CTCCAG CTGGAG(16/14)
216 CTGGAG
BsaWI 1004 ACCGGA [AT]^CCGG[AT]
1051 ACCGGA
BseRI 92 GAGGAG GAGGAG(10/8)
642 GAGGAG
BsrI 627 ACTGG ACTGG(1/-1)
929 CCAGT
BstF5I 228 CATCC GGATG(2/0)
681 CATCC
BstXI 422 CCAACACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
779 CCACCTGGCTGG
CfoI 64 GCGC GCG^C
561 GCGC
EaeI 980 TGGCCG [CT]^GGCC[GA]
1034 TGGCCA
EcoRV 25 GATATC GAT^ATC
815 GATATC
EcoT14I 200 CCAAGG C^C[AT][AT]GG
1019 CCAAGG
FokI 228 CATCC GGATG(9/13)
681 CATCC
HhaI 64 GCGC GCG^C
561 GCGC
HinP1I 64 GCGC G^CGC
561 GCGC
MaeII 522 ACGT A^CGT
542 ACGT
MseI 773 TTAA T^TAA
1043 TTAA
MslI 228 CATCCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
690 CACGCTTGTG
MspA1I 572 CAGCTG C[AC]G^C[GT]G
939 CCGCTG
NciI 845 CCCGG CC^[GC]GG
846 CCGGG
NlaIII 499 CATG CATG^
978 CATG
PstI 16 CTGCAG CTGCA^G
82 CTGCAG
SfcI 16 CTGCAG C^T[GA][CT]AG
82 CTGCAG
StyI 200 CCAAGG C^C[AT][AT]GG
1019 CCAAGG
Tru9I 773 TTAA T^TAA
1043 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI BanII BbvI
BfaI BmyI BsaJI BsiYI BslI
BsmAI Bsp1286I BssKI BstNI Cac8I
Cfr13I DpnII EcoRII Fnu4HI HaeIII
HapII HpaII HphI ItaI MaeI
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIV RsaI Sau3AI
Sau96I ScrFI TseI TspRI