Restriction map for
pF1KB3450
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AvrII BamHI BanI BbeI BbrPI
BbsI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmI Bsp1407I BspEI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII CpoI DraI DraIII
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HaeII
HincII HindII HindIII HpaI KasI
KpnI MaeI MamI MfeI MluI
MroI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SalI SapI SbfI
ScaI SfoI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp509I Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcyI 10 GACGCC G[GA]^CG[CT]C
AfaI 577 GTAC GT^AC
AviII 494 TGCGCA TGC^GCA
BbvCI 671 CCTCAGC CCTCAGC(-5/-2)
BcgI 602 GCACTGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 239 AGATCT A^GATCT
BsaHI 10 GACGCC G[GA]^CG[CT]C
BseRI 235 GAGGAG GAGGAG(10/8)
BsiEI 114 CGGCCG CG[GA][CT]^CG
BsmBI 612 CGTCTC CGTCTC(1/5)
BspDI 655 ATCGAT AT^CGAT
BspHI 641 TCATGA T^CATGA
BstAPI 376 GCATTTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 239 AGATCT [GA]^GATC[CT]
ClaI 655 ATCGAT AT^CGAT
DrdI 811 GACCACCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 114 CGGCCG C^GGCCG
EarI 247 CTCTTC CTCTTC(1/4)
EclXI 114 CGGCCG C^GGCCG
Eco52I 114 CGGCCG C^GGCCG
EcoNI 762 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 22 GCGGG CCCGC(4/6)
FspI 494 TGCGCA TGC^GCA
HgaI 10 GACGC GACGC(5/10)
Hin1I 10 GACGCC G[GA]^CG[CT]C
Ksp632I 247 CTCTTC CTCTTC(1/4)
MaeII 407 ACGT A^CGT
MflI 239 AGATCT [GA]^GATC[CT]
NaeI 352 GCCGGC GCC^GGC
NgoMIV 352 GCCGGC G^CCGGC
PvuII 863 CAGCTG CAG^CTG
RcaI 641 TCATGA T^CATGA
RsaI 577 GTAC GT^AC
SacI 146 GAGCTC GAGCT^C
SexAI 364 ACCAGGT A^CC[AT]GGT
SfcI 741 CTACAG C^T[GA][CT]AG
SfiI 37 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 12 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 304 CTCAAG C^T[CT][GA]AG
TaqI 656 TCGA T^CGA
TfiI 268 GAATC G^A[AT]TC
TthHB8I 656 TCGA T^CGA
XhoII 239 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
ApaI 28 GGGCCC GGGCC^C
36 GGGCCC
AspHI 146 GAGCTC G[AT]GC[AT]^C
426 GTGCTC
AvaI 31 CCCGGG C^[CT]CG[GA]G
677 CCCGGG
BglI 38 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
557 GCCGCTGCGGC
BpmI 860 CTCCAG CTGGAG(16/14)
232 CTGGAG
BsiHKAI 146 GAGCTC G[AT]GC[AT]^C
426 GTGCTC
BsoBI 31 CCCGGG C^[CT]CG[GA]G
677 CCCGGG
BsrFI 13 GCCGGT [GA]^CCGG[CT]
352 GCCGGC
BstXI 431 CCAAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
461 CCATCATCCTGG
CfoI 360 GCGC GCG^C
495 GCGC
Cfr10I 13 GCCGGT [GA]^CCGG[CT]
352 GCCGGC
EcoT14I 431 CCAAGG C^C[AT][AT]GG
710 CCAAGG
HhaI 360 GCGC GCG^C
495 GCGC
HinP1I 360 GCGC G^CGC
495 GCGC
MaeIII 187 GTCAC ^GT[ACGT]AC
550 GTGAC
MseI 263 TTAA T^TAA
422 TTAA
NlaIII 216 CATG CATG^
642 CATG
PleI 852 GACTC GAGTC(4/5)
836 GAGTC
SmaI 31 CCCGGG CCC^GGG
677 CCCGGG
StyI 431 CCAAGG C^C[AT][AT]GG
710 CCAAGG
Tru9I 263 TTAA T^TAA
422 TTAA
Tsp45I 187 GTCAC ^GT[GC]AC
550 GTGAC
XmaCI 31 CCCGGG C^CCGGG
677 CCCGGG
XmaI 31 CCCGGG C^CCGGG
677 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AluI BalI BanII
BbvI BcnI BmyI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI KspI MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIV SacII Sau3AI Sau96I ScrFI
SfaNI TseI TspRI