Restriction map for
pF1KB3437
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeI MaeII MamI
MfeI MluI MroI MseI MslI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AlwNI 322 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 124 GTGCTC G[AT]GC[AT]^C
AvaII 458 GGACC G^G[AT]CC
BalI 296 TGGCCA TGG^CCA
BbvCI 283 GCTGAGG CCTCAGC(-5/-2)
BsiHKAI 124 GTGCTC G[AT]GC[AT]^C
BsmFI 404 GGGAC GGGAC(10/14)
BspMI 98 ACCTGC ACCTGC(4/8)
BsrDI 158 GCAATG GCAATG(2/0)
BssKI 204 CCTGG ^CC[ACGT]GG
BssSI 447 CACGAG CACGAG(-5/-1)
BstEII 288 GGTGACC G^GT[ACGT]ACC
BstNI 204 CCTGG CC^[AT]GG
BstPI 288 GGTGACC G^GT[ACGT]ACC
EaeI 296 TGGCCA [CT]^GGCC[GA]
EarI 127 CTCTTC CTCTTC(1/4)
EcoNI 279 CCTTGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 288 GGTGACC G^GT[ACGT]ACC
EcoRII 204 CCTGG ^CC[AT]GG
Ksp632I 127 CTCTTC CTCTTC(1/4)
MluNI 296 TGGCCA TGG^CCA
MscI 296 TGGCCA TGG^CCA
MvaI 204 CCTGG CC^[AT]GG
PleI 406 GACTC GAGTC(4/5)
PstI 424 CTGCAG CTGCA^G
SapI 126 GCTCTTC GCTCTTC(1/4)
ScrFI 204 CCTGG CC^[ACGT]GG
SfcI 424 CTGCAG C^T[GA][CT]AG
TaqI 492 TCGA T^CGA
TfiI 557 GAATC G^A[AT]TC
Tsp45I 289 GTGAC ^GT[GC]AC
Tsp509I 541 AATT ^AATT
TthHB8I 492 TCGA T^CGA
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# TWO CUT ENZYMES ( 17 enzymes) #
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AluI 13 AGCT AG^CT
326 AGCT
BanII 177 GGGCTC G[GA]GC[CT]^C
216 GGGCTC
BsiYI 147 CCTCACGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
279 CCTTGCTGAGG
BslI 147 CCTCACGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
279 CCTTGCTGAGG
BsrI 414 CCAGT ACTGG(1/-1)
461 CCAGT
BstYI 62 GGATCT [GA]^GATC[CT]
92 GGATCT
Cfr13I 458 GGACC G^G[ACGT]CC
466 GGCCC
EcoT14I 293 CCTTGG C^C[AT][AT]GG
392 CCAAGG
HaeIII 297 GGCC GG^CC
466 GGCC
HinfI 406 GACTC G^A[ACGT]TC
557 GAATC
HphI 288 GGTGA GGTGA(8/7)
563 TCACC
MaeIII 289 GTGAC ^GT[ACGT]AC
371 GTTAC
MflI 62 GGATCT [GA]^GATC[CT]
92 GGATCT
Sau96I 458 GGACC G^G[ACGT]CC
466 GGCCC
SfaNI 362 GCATC GCATC(5/9)
475 GCATC
StyI 293 CCTTGG C^C[AT][AT]GG
392 CCAAGG
XhoII 62 GGATCT [GA]^GATC[CT]
92 GGATCT
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AlwI BbvI BmyI BsaJI Bsp1286I
BstF5I DdeI DpnII Fnu4HI FokI
ItaI MboI MboII MnlI MwoI
NdeII NlaIII Sau3AI TseI TspRI