Restriction map for
pF1KB3431
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BcnI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraII DraIII EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI HphI KasI
KpnI Ksp632I KspI MaeIII MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NarI NciI
NdeI NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AlwI 313 GGATC GGATC(4/5)
AspHI 40 GTGCTC G[AT]GC[AT]^C
AvaII 238 GGTCC G^G[AT]CC
BanII 516 GGGCTC G[GA]GC[CT]^C
BbvI 658 GCTGC GCAGC(8/12)
BciVI 541 GGATAC GTATCC(6/5)
BglI 23 GCCCTGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 11 GAGACC GGTCTC(1/5)
BsiHKAI 40 GTGCTC G[AT]GC[AT]^C
BsiYI 155 CCCTACGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 155 CCCTACGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 491 GCATTC GAATGC(1/-1)
BspMI 399 ACCTGC ACCTGC(4/8)
BsrFI 28 GCCGGC [GA]^CCGG[CT]
BsrI 535 CCAGT ACTGG(1/-1)
BstAPI 403 GCACTTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 647 CCATGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 313 GGATCT [GA]^GATC[CT]
Cfr10I 28 GCCGGC [GA]^CCGG[CT]
Cfr13I 238 GGTCC G^G[ACGT]CC
DrdI 502 GACGGCTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 647 CCATGG C^C[GA][CT]GG
Fnu4HI 658 GCTGC GC^[ACGT]GC
HapII 29 CCGG C^CGG
HgaI 482 GCGTC GACGC(5/10)
HindIII 221 AAGCTT A^AGCTT
HinfI 379 GAATC G^A[ACGT]TC
HpaII 29 CCGG C^CGG
ItaI 658 GCTGC GC^[ACGT]GC
MflI 313 GGATCT [GA]^GATC[CT]
MseI 551 TTAA T^TAA
MslI 648 CATGGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 29 CCGG C^CGG
NaeI 28 GCCGGC GCC^GGC
NcoI 647 CCATGG C^CATGG
NgoMIV 28 GCCGGC G^CCGGC
PshAI 142 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau96I 238 GGTCC G^G[ACGT]CC
SpeI 528 ACTAGT A^CTAGT
TaqI 74 TCGA T^CGA
TfiI 379 GAATC G^A[AT]TC
Tru9I 551 TTAA T^TAA
TseI 658 GCTGC G^C[AT]GC
TthHB8I 74 TCGA T^CGA
XhoII 313 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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AciI 133 CCGC CCGC(-3/-1)
58 GCGG
BbsI 253 GAAGAC GAAGAC(2/6)
417 GTCTTC
BfaI 430 CTAG C^TAG
529 CTAG
BmyI 40 GTGCTC G[AGT]GC[ACT]^C
516 GGGCTC
BpmI 34 CTCCAG CTGGAG(16/14)
607 CTGGAG
BpuAI 253 GAAGAC GAAGAC(2/6)
417 GTCTTC
BsaJI 442 CCTTGG C^C[ACGT][ACGT]GG
647 CCATGG
BsgI 401 CTGCAC GTGCAG(16/14)
640 GTGCAG
BsmAI 11 GAGAC GTCTC(1/5)
454 GAGAC
Bsp1286I 40 GTGCTC G[AGT]GC[ACT]^C
516 GGGCTC
BstF5I 447 GGATG GGATG(2/0)
651 GGATG
DdeI 352 CTAAG C^T[ACGT]AG
507 CTTAG
EcoT14I 442 CCTTGG C^C[AT][AT]GG
647 CCATGG
FokI 447 GGATG GGATG(9/13)
651 GGATG
HaeIII 31 GGCC GG^CC
198 GGCC
MaeI 430 CTAG C^TAG
529 CTAG
MaeII 159 ACGT A^CGT
363 ACGT
NlaIII 151 CATG CATG^
648 CATG
SfcI 260 CTACAG C^T[GA][CT]AG
275 CTACAG
StyI 442 CCTTGG C^C[AT][AT]GG
647 CCATGG
Tsp509I 470 AATT ^AATT
549 AATT
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI BseRI BssKI BstNI Cac8I
DpnII EcoRII MboI MboII MnlI
MvaI MwoI NdeII Sau3AI ScrFI
TspRI